We narrowed to 855 results for: NTS

...result to a reference sequence to confirm backbone elements.   Confirming the gene/insert by aligning to NCBI...origin of replication or other common backbone elements. Since we’ve successfully grown the plasmid in... about our sequencing results in the Depositor Comments section at the bottom of the plasmid page. Viral...

...of the mutations engineered into each of their parents. Here’s how to cross mice, using a simple example... all of your desired alleles to conduct your experiments. You should also maintain your parental strains...contain a mix of genetic material from each of the parents. If both of the mutations that you are trying to...

...introduction, methods, results, conclusions, and acknowledgements sections. Some other additions you might wish...present at your poster at all times, so other participants can identify you. You may also wish to make ...the introduction, methods, conclusions, and acknowledgements sections are inserted. The advantage of this...

...Messina et al, 2016). We quickly assembled a team of students, postdocs and professors to move the project forward... member of the team to accomplish so that many elements of the project were able to move forward quickly...of Zika Virus Using Programmable Biomolecular Components." Cell (2016). PubMed PMID: 27160350. Find Plasmids...

...discussions are becoming a ubiquitous format for events and sessions in scientific conferences. They can...a table. It adds a formality and distance that prevents interaction with each other and the audience. ... inviting interesting friends and colleagues to events for this purpose. If possible, make sure the panelists...

...difficult to ensure comparable expression of all components of Cascade for maximum efficiency of the process...During DNA repair, there is a chance that the two breakpoints will repair without the section between the two... mentioned issues with expressing all Cascade components can make Cas9 easier to work with than Cas3. ...

... antibodies for aminoacyl tRNA synthetases Nanocompartments for yeast bioproduction pathways The CRISPR...with previous research, which required protein compartments to co-localize multiple enzymes in a reaction...expressing GCaMP6f with this promoter. Image calcium transients in PV interneurons with AAV-S5E2-GCaMP6f are ...

...to be inert, to not produce artifacts in your experiments. Considerable effort has gone into making FPs...biosensors were made with CFP and YFP, cyan and yellow variants of EGFP equally capable of dimerizing as EGFP....for Your Needs Get Tips for Using FRET in Your Experiments Additional Resources on Addgene.org Browse ...

...find more detailed information about the plasmid contents? The middle section of our plasmid pages contain...information you'll need to use the plasmid in your experiments: Backbone: The starting vector that the depositing...from Addgene!).  It also contains links to any agreements under which the plasmid is distributed.  All ...

...virus, we will see that they are very central determinants of how AdVs behave, and in fact, of how adenoviral...present in the HEK293T cell line used (which have fragments of the AdV genome in them, including the E1 region...intracellularly-exposed proteins and downstream cellular events, all recruit strong innate and adaptive immune ...