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We narrowed to 842 results for: NTS

Showing: 601 - 610 of 842 results

  1. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    Published
    March 31, 2020, 1:15 p.m.
    ...copy number of the plasmid increases, increased amounts of these antisense RNAs inhibit plasmid replication...The concentration of negative regulation system elements in the cell is artificially high for both plasmids... uncommon, or perhaps a synthetic biologist who wants to develop a plasmid-based tool that is easy to ...

  2. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

    Type
    Blog Post
    Published
    April 2, 2019, 1:44 p.m.
    ...regularly spaced denticles (bristles) outline the segments of the Drosophila embryo. In flies with non-functional...1997). We have a lot of plasmids that contain components of the SB transposon system including those from...of this gene name but the larval gut of bagpipe mutants vaguely resemble a bagpipe and historically, bagpipes...

  3. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    Published
    Nov. 1, 2018, 12:35 p.m.
    ...ccdB gene die off and a higher percentage of transformants contain the ccdB-less construct. Other toxin-antitoxin...described, the hok/sok systems consist of three components: Host killing (hok), a long-living toxin that...selective pressure is necessary or for long-term experiments. They’ve also helped researchers design and optimize...

  4. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ...what does this mean for a molecular biologist who wants to express genes from one organism in another? Let...organism that contains expression limiting regulatory elements or use a non-canonical code (Gustafsson et al....optimization should not be used in some in vivo experiments and in biotechnology and therapeutics (Mauro ...

  5. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...community obtained high-quality data for our DNA-based reagents. Not only do we extract as much information as...system that we chose needed to fulfill certain requirements: Customizable to our specific needs Modern ...-well plate basis, but for rapid testing and refinements, we specified a select small number of input ...

  6. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    Published
    Jan. 24, 2023, 2:15 p.m.
    ...silencing required addition of chromatic-opening agents to boost expression to appreciable levels. We wanted...expression and robustness without chromatin-opening agents. It ended up being very straightforward to engineer...for improved purity and contrast. For in vivo experiments, simply induce injected engineered cells via ...

  7. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ...remember where you read particular studies or experiments. Later, you can easily convert the citation style...much you can get done between office hours and experiments. A comment about citing: I wasn’t sure if I should... be revolutionary, but you could include a few comments on where you think the field is going or what ...

  8. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    Published
    Nov. 10, 2016, 3:30 p.m.
    ...because ‘antibiotic’ merely means that the drug prevents the growth of a living organism. As for multi-...making them more difficult to treat in the future. Patients not finishing the whole course of antibiotics ... can utilize tactics like rotating antibiotic treatments, so that exposure to each antibiotic is minimized...

  9. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ...rounded out the top four average video abstract view counts.” For researchers looking to garner more attention...can be shared by research scientists, faculty, students, private sector researchers, or pretty much anyone...the study, or even review Facebook and Twitter comments. On We Share science, video usurps the written...

  10. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ...sequential homologous recombination events, but because these events are rare, each requires selection....efficient method to generate precise, unmarked mutants, but it frequently requires considerable optimization...
Showing: 601 - 610 of 842 results