We narrowed to 855 results for: NTS

...virus, we will see that they are very central determinants of how AdVs behave, and in fact, of how adenoviral...present in the HEK293T cell line used (which have fragments of the AdV genome in them, including the E1 region...intracellularly-exposed proteins and downstream cellular events, all recruit strong innate and adaptive immune ...

...CRISPR affinity purification in situ of regulatory elements (CAPTURE) (Liu et al., 2017). This system utilizes...techniques, this method also has great potential for improvements and adaptations that can provide an increased..."Efficient sequence‐specific isolation of DNA fragments and chromatin by in vitro enCh IP technology using...

... that introduced an ancestry bias into their experiments instead of a true correlation.   It turned out...five-team response to address this issue, but it still points to a far larger and more complex problem endemic...contributes to false negatives in CRISPR-based experiments with varying burden across ancestries. Sean A...

...remember where you read particular studies or experiments. Later, you can easily convert the citation style...much you can get done between office hours and experiments. A comment about citing: I wasn’t sure if I should... be revolutionary, but you could include a few comments on where you think the field is going or what ...

...copy number of the plasmid increases, increased amounts of these antisense RNAs inhibit plasmid replication...The concentration of negative regulation system elements in the cell is artificially high for both plasmids... uncommon, or perhaps a synthetic biologist who wants to develop a plasmid-based tool that is easy to ...

...silencing required addition of chromatic-opening agents to boost expression to appreciable levels. We wanted...expression and robustness without chromatin-opening agents. It ended up being very straightforward to engineer...for improved purity and contrast. For in vivo experiments, simply induce injected engineered cells via ...

...community obtained high-quality data for our DNA-based reagents. Not only do we extract as much information as...system that we chose needed to fulfill certain requirements: Customizable to our specific needs Modern ...-well plate basis, but for rapid testing and refinements, we specified a select small number of input ...

...regularly spaced denticles (bristles) outline the segments of the Drosophila embryo. In flies with non-functional...1997). We have a lot of plasmids that contain components of the SB transposon system including those from...of this gene name but the larval gut of bagpipe mutants vaguely resemble a bagpipe and historically, bagpipes...

...what does this mean for a molecular biologist who wants to express genes from one organism in another? Let...organism that contains expression limiting regulatory elements or use a non-canonical code (Gustafsson et al....optimization should not be used in some in vivo experiments and in biotechnology and therapeutics (Mauro ...

...ccdB gene die off and a higher percentage of transformants contain the ccdB-less construct. Other toxin-antitoxin...described, the hok/sok systems consist of three components: Host killing (hok), a long-living toxin that...selective pressure is necessary or for long-term experiments. They’ve also helped researchers design and optimize...