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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 842 results for: NTS

Showing: 641 - 650 of 842 results

  1. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    Published
    May 2, 2023, 1 p.m.
    ...Like promoter traps, enhancer traps lack enhancer elements and require the trapping of endogenous enhancers...to generate a collection of monitored genes and mutants - viral integration, transposon, and many more!...

  2. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    Published
    Feb. 21, 2023, 2:15 p.m.
    ...reproducibility of scientific data depends on validated reagents along with clear documentation, so we were excited...literature, or to decide which line is best for your experiments. You can also use the Cellosaurus STR similarity...

  4. The Blue Flame Award: Celebrating Addgene's Most Requested Depositors

    Type
    Blog Post
    Published
    Nov. 14, 2016, 3:30 p.m.
    ...powerhouse depositors who have generously shared their reagents freely with the research community. Sharing is...logistics and facilitate all material transfer agreements, thereby freeing up time for our depositors to...

  6. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    Published
    Aug. 19, 2014, 3:39 p.m.
    .... To help you find the vector that fits your experiments, I've described below some of the most frequently...backbone. Or just ask –  leave a message in the comments below and an Addgenie will help you find what ...

  7. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    Published
    March 13, 2014, 4:08 p.m.
    ...double nickase for my CRISPR genome engineering experiments? A1: When assessing which nickase type to use...use for your CRISPR genome engineering experiments, consider that wildtype Cas9 with optimized chimeric...

  8. Choosing a Good Mentor for Scientists

    Type
    Blog Post
    Published
    Jan. 16, 2014, 2:56 p.m.
    ...scientist. It is shocking how little pre-work most PhD students and postdocs do to ensure the advisors they choose... than once to get a full picture and listen for hints at the same concerns from multiple people. Listen...

  9. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    Published
    Nov. 25, 2013, 10:12 p.m.
    ...bacteriophages did not come to light until 2007. Initial experiments exposed S. thermophilus to predatory phages to... Cas proteins snip the foreign DNA into small fragments approximately 30bp in length and paste them into...

  10. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    Published
    Sept. 21, 2021, 1:15 p.m.
    ...to 266 μg/mL. The reaction only required three components: a master mix, the DNA of interest, and cell ...samples, were engineered and adapted for RNAi experiments for mammalian cell lines. The Turner Lab Human...
Showing: 641 - 650 of 842 results