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We narrowed to 855 results for: NTS

Showing: 661 - 670 of 855 results
  1. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    Published
    Nov. 25, 2013, 10:12 p.m.
    ...bacteriophages did not come to light until 2007. Initial experiments exposed S. thermophilus to predatory phages to... Cas proteins snip the foreign DNA into small fragments approximately 30bp in length and paste them into...
  2. Viral Vectors 101: Transductions

    Type
    Blog Post
    Published
    April 30, 2024, 1:15 p.m.
    ...nucleic acid will be flanked by viral packaging elements that will ensure the virus gets produced, and ...What is a Virus? Viral Vectors 101: Viral Vector Elements ...
  3. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    Published
    Sept. 21, 2021, 1:15 p.m.
    ...to 266 μg/mL. The reaction only required three components: a master mix, the DNA of interest, and cell ...samples, were engineered and adapted for RNAi experiments for mammalian cell lines. The Turner Lab Human...
  4. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    Published
    Dec. 19, 2019, 2:40 p.m.
    ...transduction patterns empirically for their own experiments. To this end, we offer specific control AAV in...spatiotemporal resolution required for precise in vivo measurements of dopamine (113049, 113050) or norepinephrine...
  5. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    Published
    Jan. 25, 2018, 2:09 p.m.
    ...lysine residue within the AviTag. However, large amounts of the target protein are typically required due... site could be used for plasmid version 2.0? Instruments such as an Octet may require less in vivo-biotinylated...
  6. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    Published
    May 9, 2019, 1:15 p.m.
    ... with different CellTag libraries at multiple timepoints during reprogramming. These library-specific ... with different CellTag libraries at three time points during the 28 day study: prior to reprogramming...
  7. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    Published
    March 26, 2019, 1:40 p.m.
    ...jGCaMP7 (keep reading) and will be releasing more variants in the next few months (see “coming soon”). The... can also be used as the control vectors for experiments encoding a fluorescent-tagged molecular tool....
  8. Summer SciComm: Science Storytelling

    Type
    Blog Post
    Published
    Sept. 16, 2022, 1:15 p.m.
    ...communicators also use the hero’s journey, stories from patients or other affected people, man/animal vs nature... the Addgene blog SSCS: Cognitive Load SSCS: Preprints Intro to our Summer SciComm Series  ...
  9. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    Published
    April 7, 2015, 4:21 p.m.
    ...metabolic flux. The dCas9 protein and orthogonal variants can be used as regulatory ON/OFF master switches...knowledge base to describe aptamers and SELEX experiments. Database, bas006 (2012). PubMed. For a stepwise...
Showing: 661 - 670 of 855 results