We narrowed to 842 results for: NTS
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TypeBlog PostPublishedApril 27, 2023, 1:15 p.m.... Unlike traditional dye-based neuronal tracing reagents, AAV particles require receptor-mediated entry...neurons. When using this serotype to conduct experiments with chemogenetic vectors, this property must...
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Plasmids 101: Screening Strategies Used in Plasmid Cloning
TypeBlog PostPublishedSept. 15, 2020, 1:15 p.m....have to weed through fewer colonies after your experiments. As a common example, a selection will leave ...get started, you will first need a primer that complements your plasmid sequence. Start with a backbone-... -
AAV Q&A with Tim Miles
TypeBlog PostPublishedMay 17, 2022, 1:15 p.m....diversity in Vector Assisted Spectral Tracing (VAST) experiments using PHP.eB in the brain give some insight into...Xinhong Chen, and Miggy Chuapoco, and to all our participants for their time and excellent questions. ... -
Building and Validating Recombinant Antibodies: A Case Study
TypeBlog PostPublishedApril 5, 2022, 1:20 p.m....validation pipeline. (B) Schematic shows the separate elements of the R-mAb expression plasmid involved in coexpression...R-mAb) expression plasmids, allowing for important reagents that could have been lost to continue to be available... -
Hot Plasmids - February 2022
TypeBlog PostPublishedFeb. 22, 2022, 2:15 p.m.... is advantageous to Cas9’s larger expression requirements (Figure 1), but Cas12a has generally been underutilized...low-pressure teaching opportunity for lab members or students new to protein expression and purification. ... -
Antibodies 101: Isotypes
TypeBlog PostPublishedOct. 19, 2021, 1:15 p.m....antibodies through cells and into different body compartments, such as into mucus, tears, or milk. When using...switches to producing IgE antibodies, the gene segments for IgM, IgD, and IgG are deleted. ... -
Antibodies 101: Monoclonal Antibodies
TypeBlog PostPublishedJune 29, 2021, 1:15 p.m....just getting started using antibodies in your experiments, you may be curious about all of the different... are first injected with a small peptide that represents a short, unique amino acid sequence found in ... -
A Quick Guide to a Career in Software Product Management
TypeBlog PostPublishedNov. 24, 2020, 2:15 p.m.... into the key takeaway themes and customer pain points we’ve identified. I can’t wait to send it out. ...work on next to help alleviate any customer pain points. Many thanks to our guest blogger Rachel Rubinstein... -
Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens
TypeBlog PostPublishedFeb. 24, 2015, 7:50 p.m.... of endogenous genes and it consists of three components: 1. A nucleolytically inactive Cas9-VP64 fusion...considering the use of pooled libraries in your experiments, Addgene recommends that you review the steps... -
It’s Bioelectric! An Exciting Interdisciplinary Field of Research
TypeBlog PostPublishedOct. 29, 2019, 2:52 p.m....genome engineering. Planarian worms cut into three segments “remember” which side should grow a head and a...bioelectric signaling networks: exploiting voltage gradients for control of growth and form." Annual review...