We narrowed to 821 results for: LAS

... work out of the plasmid creation process. Each module of Fragmid represents a class of components, such...restriction digest, and then plasmid sequencing if you can. To quote Doench, “Whole plasmid sequencing is just ...correctly banded clones for plasmid sequencing and found 98% of the plasmids were perfectly correct. Because... Mammalian Plasmid pRDA_791 (contains only orio and ampR) Drosophilia Plasmid pRDB_051 (for...the assembled plasmid. It shows you each component, with the ID of the component plasmid, component name...the component plasmids on the Addgene website. Step 4: Naming your assembled plasmid and generating... is to your liking, your last step, Step 4, is to give the assembled plasmid a name and generate the vector...

...replication. In general, these plasmids tend to be low copy number. Conversely, plasmids that can initiate DNA ...growth against sustainability. Plasmid DNA replication and regulation Plasmids must also strike a balance:...a given plasmid has been reached, negative feedback circuits reduce replication of the plasmid to balance...balance the total plasmid number.  Let’s consider pSC101. This plasmid is stringently controlled and has...promotes initiation at the plasmid ori, after which replication of the plasmid proceeds (and terminates)...tool to stringently regulate plasmid replication. For example, the plasmid ColIb-P9 encodes the protein...All plasmids rely on their host cell's replication machinery in order to replicate—but not always to ...

... sequence using BLAST2. See this blog post for tips on using BLAST to verify plasmids. Analyzer guide...SnapGene powered plasmid maps at Addgene  Three factors make our SnapGene powered plasmid maps far easier...easier for you to share plasmid information with colleagues, or explain plasmid features to a lab member... on the main plasmid page under the "Resource Information" section. Analyze your plasmid sequence For ...and “Sequence” tabs. BLAST Use the Basic Local Alignment Search Tool (BLAST) via the NCBI website ...between a given sequence and nucleotide (BLASTN) or protein (BLASTX) sequence databases. Additionally, align...over from our users: "Please, please improve your plasmid maps!" After thoughtful design, vetting, and tweaking...

...protocols, and, of course, plasmids. Today we’re trying a new way to present plasmid info with a new segment...The Hot Plasmids Segment. Click on the player below to listen to a quick (~5 min) Hot Plasmids podcast... new plasmid technologies from one of our recent newsletters. Listen to the entire Hot Plasmids episode...greatest plasmids through this podcast segment. Below, we break down each of the Hot Plasmids segments...articles on the Hot Plasmid webpage. We hope you enjoy this new way to learn about plasmid technologies. If...comments about the Hot Plasmids Podcast Segment, or have recommendations for new Hot Plasmids topics, let us ... comments section below. Hot plasmids covered in this segment Hot Plasmid 1: CRISPR X from Michael Bassik's...

...most request plasmid in 2016 was... pSpCas9(BB)-2A-Puro (PX459) V2.0       Similar to last year, the top...for sure, as is obvious from our most requested plasmid, SpCas9 is still going strong as the basis for ... top requested plasmid from 2016 is an expression vector for CRISPR nuclease, SpCas9 from the Zhang Lab... of mammalian cell types. Purpose  SpCas9 This plasmid expresses Streptococcus pyogenes (Sp) Cas9 from...sequence as directed by a gRNA. 2A-Puro In this plasmid, SpCas9 is fused to the puromycin resistance gene...resistance gene enables selection of cells with the plasmid post transfection. The 2A "self cleaving" peptide... What's not in the name? Not included in this plasmid's name are some additional features. The first and...

...mutation” field on the plasmid page (see plasmid 87904 for example). As many plasmids available from Addgene...blog Plasmids 101: protein expression Plasmids 101: E. coli strains for protein expression Plasmids 101...codon optimized by ordering plasmids containing them from Addgene. If a plasmid at Addgene contains a gene...

...a million plasmids would fill? Two weeks ago, Addgene edged passed our one million plasmids shared milestone...ending the day with 1,000,002 plasmids shipped. Reaching one million plasmids shared might have you wondering...how many plasmids leave Addgene headquarters every day. That would be an average of 673 plasmids each day...to packing and shipping. Every single day. 673 plasmids shipped daily may be easy to picture (see the ...celebratory lunch, it’s hard to picture one million plasmids. How much space would one million bacterial stabs...Leadership team in all their photo booth glory! #1millionplasmids On to the next million! @CarolineBME @AllostEricSite...post, we looked at how long it takes to make one plasmid and how much time Addgene has saved researchers...

...for your work. Species Antibody Subclass Nanobody Addgene Plasmid Rabbit N/A* TP897 pTP1183 (1x... one IgG subclass.   Express the Nanobody In Bacteria: Table 2 also has a link to the plasmid you’ll need... How to Use the Nanobody Toolbox Find Nanobody Plasmids Structural comparison As the name suggests, ...different species, or at least from different IgG subclasses, so that secondary antibodies exclusively recognize...Must use different species and/or different IgG subclasses of 1° for multi-color staining Can be expensive...limited cross-reactivity between different IgG subclasses and different species, nanobodies are useful ...require using primary antibodies from different IgG subclasses. Instead, you can pre-incubate individual primary...

...Browse our Plasmids 101 blog posts Read blog posts on plasmid cloning Read about different plasmid elements...to the backbone plasmid; a starting point could be a molar ratio of 1:2 (plasmid:insert), but this parameter...colonies screened might not contain the correct plasmid. There’s a lot that can go wrong! With all the ...cloning project, and hopefully obtain your coveted plasmid with no substantial delays. Design, design, design...spend some time outlining the construction of the plasmid and all the steps you will have to take. Decide...you chose, and verify that the sequence of the plasmid you are working with is correct. It's also best...ways you can assemble the different parts of a plasmid based on the cloning strategy you followed. While...

...popular eBook, Plasmids 101, is now available!   Plasmids 101 covers everything from plasmids parts and ...word for it!  “When people want to learn about plasmids, they don’t go to Wikipedia; they go to Addgene...