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We narrowed to 599 results for: REV;

Showing: 61 - 70 of 599 results

  1. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    Published
    Nov. 10, 2022, 2:15 p.m.
    ...you. Screen variations Forward and reverse screens Forward and reverse screens involve manipulating or ...manipulation of the population. This is contrary to reverse screens, where there is removal of known factors...genes. This is a perfect example of an unbiased, reverse screen – all known genes are assayed for their ...types of screens are very similar to forward and reverse screens, except that they begin with a uniform ...manipulations which either enhance or suppress the previously defined disease phenotype. These screens employ... same downstream manipulations as a forward or reverse screen (CRISPR, drug, etc.), just with an added...

  2. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    Published
    July 22, 2014, 5:46 p.m.
    ... Learn more about Revolution Bioengineering Follow RevBio on Twitter Like RevBio on Facebook Read...Keira Havens, co-founders of Revolution Bioengineering. Read their previous blog post about how they started...the public in an open, responsible manner. At Revolution Bio, we are addressing this challenge directly...project. We’ve shared some of the concerns that Revolution Bio had to address as we developed the color-...

  3. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    Published
    Oct. 9, 2018, 12:21 p.m.
    ... tips for a successful postdoc fellowship. 1. Reverse engineer success On Day 1 of your postdoctoral ...these goals. Planning is often more easily done in reverse. Assuming you want to become competitive for a ... typically takes 6-12 months depending on the revisions and the amount of time spent shopping a manuscript...conversations with senior investigators. Discussions can reveal mutual scientific interests, complementary perspectives...more positive grant application and manuscript reviews, etc. You'll also meet future collaborators and...impressions on potential manuscript or fellowship reviewers, expose your research to search committees, and...Seek opportunities to write manuscript drafts, reviews, cover letters, fellowships, etc. Second, get constructive...

  4. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type
    Blog Post
    Published
    Jan. 11, 2017, 3:30 p.m.
    ...decisions to alter postdoc salaries - in some cases reversing these decisions entirely. At Future of Research...resource leading up to this date, as discussed in a previous Addgene blogpost and more recently in a paper ...initially cancelled their plans, but have since reversed their position and decided to allow individual... in Boston also just announced that they were reversing their plans to cancel raises). A further 9 institutions...What will these further changes mean? The abrupt reversal in salary raises at institutions will likely have...where salaries had already been raised higher previously and all 6,000+ postdocs at the University of ...Additional Resources on the Addgene Blog Read Gary's Previous Article: Changing Labor Laws Bring Increased Postdoc...

  5. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    Published
    July 27, 2023, 1:15 p.m.
    ... have its structural proteins expressed from a reverse-transcribed piece of DNA called a “provirus.” This...target cell” (or host cell) by the viral protein reverse transcriptase (RT). In the case of a natural virus...virus, both the protein and the genetic code for reverse transcriptase are packaged (Wu et al., 1997) inside...vector! For viral vectors, only the protein for reverse transcriptase (RT) is present in the capsid, as...translation of proteins because they integrate this reverse-transcribed DNA into the host cell’s genome and... in step 2 during uncoating. After uncoating, reverse transcription follows the release of the two strands...encoded by complex retroviruses. Microbiological Reviews, 56(3), 375–394. https://doi.org/10.1128/MR.56.3.375...

  6. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

    Type
    Blog Post
    Published
    March 17, 2016, 2:30 p.m.
    ...reporter gene transcription by the EBOV polymerase (reviewed in 2, 5). The minigenome is capable of being replicated... many nonsegmented negative sense RNA viruses (reviewed in 2, 3). Other important components of the Zaire...replication and transcription: NP, VP35, VP30, and L (reviewed in 5). The RNA genome is encapsidated by the nucleoprotein...arguing against this approach. First, this would prevent the study of the proteins independently from each...Central PMCID: PMC103829. 2. Conzelmann, K. K. "Reverse genetics of mononegavirales." Biology of negative...negative strand RNA viruses: The power of reverse genetics. Springer Berlin Heidelberg, 2004. 1-41. PubMed...replication competent virus-like particles and beyond: reverse genetics systems for filoviruses and other negative...

  7. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    Published
    Nov. 19, 2020, 2:15 p.m.
    ...individuals with Rett syndrome experience regression of previously acquired skills such as speech, coordination,...landmark paper in Science showed that dramatic reversal of disease symptoms in mice is possible (Guy et... halted the neurological damage but completely reversed it, regardless of age or symptom severity. This... exciting finding injected hope that a similar reversal may be possible for children and adults with Rett...RNA trans-splicing, and protein replacement to reverse the impacts of this disease.  As a non-degenerative...non-degenerative, monogenic disease that has shown reversibility of symptoms in animal models, Rett syndrome is ...Guy J, Gan J, Selfridge J, Cobb S, Bird A (2007) Reversal of Neurological Defects in a Mouse Model of Rett...

  8. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    Published
    Nov. 10, 2016, 3:30 p.m.
    ... any preventive measure designed to help keep you from getting sick can be helpful in preventing the spread... on preventing the spread of antibiotic resistant bacteria, check out this resource on preventing antibiotic...because ‘antibiotic’ merely means that the drug prevents the growth of a living organism. As for multi-...standpoint, we can all work together to try and prevent outbreaks of drug resistant bacteria within communities...standards. Handwashing is an essential component of preventing infection, as is cleaning and disinfecting frequently...

  9. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ... and the promoter to prevent leaky expression of this resistance. Unlike previous systems, pUCXKT does...can do so in a very specific, precise manner. Previously, scientists have been successful in regulating...enzymes (e.g. nucleases, recombinases) which irreversibly excise or invert a specific chunk of DNA. However...deposited a set of 11 phage integrases which act irreversibly on their cognate attB/attP sites with no cross-talk...subcellular localization in mammalian cell culture and reversible control of signalling pathways. These LIDs are... powerful tools which allow you to easily and reversibly switch off and on your favourite signalling pathways...the MCS has several options) and a pUCX-specific reverse primer containing the missing codons from the antibiotic...

  10. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    Published
    Oct. 17, 2019, 12:28 p.m.
    ... have taken researchers to the threshold of a revolution in modern neuroscience, an era in which technologies...antisense orientation with respect to the promoter to prevent its expression, and is flanked (“floxed”) by two...electroencephalography evidences of arousal in mice previously subjected to steady-state general anesthesia ...behaviors. The high spatio-temporal precision and reversible modulation of FLEx vectors combined with the ...functions, and molecular mechanisms." Chemical reviews 114.1 (2013): 126-163. PubMed PMID: 24364740. PubMed...development and application of optogenetics." Annual review of neuroscience 34 (2011). PubMed PMID: 21692661...of cre-loxp site-specific recombination." Annual review of biophysics and biomolecular structure 30.1 (...
Showing: 61 - 70 of 599 results