We narrowed to 583 results for: BASE;

...interaction approaches that of GST-glutathione. Biotin-based immobilization for kinetic analysis Proteolytic ...bacteria. Our initial attempt to create a plasmid based on the biotin ligase BirA backbone (Eric Campeau...

...Topoisomerase based cloning (TOPO cloning) is a DNA cloning method that does not use restriction enzymes...polymerase has an error rate of about 1 in 3,500 bases. Typically polymerases with proofreading functionality... relies on the basic ability of complementary basepairs adenine (A) and thymine (T) to hybridize and form...

...University where she is developing fluorescent protein-based biosensors for Human Milk Oligosaccharides (HMOs...production of HMOs. Current HPLC-MS and fluorophore-based technologies are too time consuming in the first...

...discriminating between neurons for light microscopy-based neuronal reconstruction. To achieve stochastic multicolor...conventional transgenic strategies. In such vector-based gene delivery systems, the stochastic expression...

... remotely can be more difficult if your work is based in the lab. If you’re unsure what to do during this...submitting a registered report, where peer review is based on the methods, not the results. Also consider using...

...long-term (AAV). If you perform a lipid-based or electrical-based transfection, most of the nucleic acid...

...handy resource for anyone looking to use antibody-based applications in their research.  Like all our eBooks...

...They also produce more than 20 other MTRIA sensors based on GPCRs recognizing various neurotransmitters, ...powerful high-throughput screen to select variants based on performance metrics specifically for two-photon...phosphorylated state of an immunoreceptor tyrosinase-based activation motif (ITAM). By fusing ITAM to an RTK...

... The HA frankenbody works just like an antibody-based probe, but is easier and cheaper to generate since...scFvs: Just like antibodies, there are many scFv-based therapies in clinical trials or already in the clinic...blog: Read about frankenbodies, an anti-HA scFv-based probe for fluorescent imaging. Learn more about ...

...Institute for Protein Innovation (IPI), a Boston-based, nonprofit protein research institute, partnered...unconjugated and recommended for use in flow cytometry. Based on the established applications of the original ...with the Boston Children’s Hospital and Harvard-based lab of Tim Springer, the founder of IPI. Caitlin...

...with the bulkiness that accompanies mispairing of bases. To reduce off-targets, you’ll want to select a ... Off-targets that come from mutations arise from base/prime editing or Cas cleavage. In the case of Cas...Resources   References Rabinowitz R, Offen D. Single-Base Resolution: Increasing the Specificity of the CRISPR-Cas...

... optogenetic tools for manipulating Rho GTPases based on BcLOV4. These single-component systems take advantage...interested in directed-evolution methods, the activity-based selection in mammalian cells used here could be ...Fromme, J. C., & Baskin, J. M. (2023). Activity-based directed evolution of a membrane editor in mammalian...

... MACS. But MACS usually only allows for sorting based on a single (lineage) marker, while fluorescence-activated...biorender.com.   Dye stains Dye stains are non-antibody-based stains that bind DNA or free amines of proteins....cytosolic and/or nuclear markers. Lastly, stains, based not on antibodies but on small molecule dyes, are...

..., especially in in vivo models, and new Cre-lox based technologies are still being developed today. In...Flpbow construct that is functionally similar to Cre-based Brainbow, but is controlled by FLP/FRT recombinase...-44. PubMed. Drosophila Brainbow: a recombinase-based fluorescence labeling technique to subdivide neural...

...conduct their hands-on experiments.  As a project-based curriculum, BioBuilder expands learning outcomes...Mary Tamer from BioBuilder! Mary Tamer is a Boston-based writer, editor, and consultant whose work is focused...

...their choice to share their fluorescent protein based research. This year, we put out another call for...fluorescence-activated cell sorting into four bins based on their eGFP:mCherry ratios. Genomic DNA from each...

...user-defined genomic region. EvolvR, a CRISPR-Cas9 based targeted mutagenesis method developed by the Dueber...changes cytidines or guanines to the other three bases, which limits the level of variation that can be...

...commonly used for the prediction of T cell epitopes based on the amino acid sequence of the antibody.  NetMHCPan...Assays to Assess Immunogenicity Risk of Antibody-Based Biotherapeutics. PloS one, 11(8), e0159328. https...

...and proud member of Crowdfight. He is currently based in Spain and works on climate change. You can follow...the coordinators at Crowdfight. She is currently based in Spain and works in the field of genetics and ...

...to altered function or maybe your favorite FRET-based biosensing technique can be applied to an important...experiments enabled by physical, software, and biology-based tools as well as collaborations between different...

...scientists can still reap the benefits of plasmid-based research using synthetic vectors and cultured mammalian..." mammalian ORIs, scientists have usurped viral-based ORIs to fill the void. These ORIs, however, require...

... more than one person, we selected the finalist based on the first time of submission.)     We love...

...predicted deleteriousness of the amino acid change based on maps of protein structure. Sometimes this provides... years, we’ve hoped that large scale laboratory-based functional experiments that classify which variants...

...by gel or by nanodrop to assemble the  fragments based on their molar ratio. Assembly of the fragments ...you can assemble the different parts of a plasmid based on the cloning strategy you followed. While most...

...Addgene depositor Alice Ting used yeast display-based directed evolution to create APEX2. They have now...without special equipment. What’s more, detection is based on color changes to the assay paper, so no special...

...modified) for optimal stability and efficacy.    Base modifications What is more stable than RNA? DNA!...gRNAs for Spatiotemporally Controlled CRSIPR-Cas-based Genomic Regulation. ACS 6, 5, 695-703 (2020). https...

...critiques or endorsements. In contrast, journal-based peer review is usually a confidential process among...ensure a manuscript’s quality. But even journal-based peer review can be prone to biases or errors, with...

...staining. Isotype controls are used in antibody-based applications including flow cytometry, immunohistochemistry...of two. There are four IgG subclasses, numbered based on their abundance in blood: IgG1, IgG2, IgG3, and...

...all be ssDNA within the viral particle. The ITRs base pair with each other to allow DNA synthesis of the...57 human adenovirus types, but most vectors are based on adenovirus 5 (Ad5).     Fig. 3 - Viral...

...mechanism for increasingly popular nucleic acid-based vaccines. These vectors provide several advantages...delivery system for human patients. Multiple viral-based gene therapies have already been approved by the...

...hours of hands-on time to make a single plasmid, based on the assumptions outlined in Table 1.   Of course...performing the experiments. This cost varies hugely based on a number of factors, but we can assume it’s at...

...that covers most things CRISPR - from nickases to base editing to pooled library screening. It also has...

...Open Hardware? Author: Benedict Diederich Course-Based Undergraduate Research Experiences (CUREs)Author...AccessibleResearcher: Ryan Mehl; Andy KarplusIBEX Knowledgebase: A Data Resource for Multiplex Tissue ImagingResearchers...YanevCellosaurus: A Cell Line Information DatabaseResearcher: Amos BairochViral Vectors 101: Systemic ...

...causes of irreproducibility with some solutions based on what I’ve learned. I hope you can apply some ...all human extracellular proteins with community-based validation. They also have plans to expand to other...with larger and larger datasets and use computer based analysis techniques, an important component of reproducibility...on the Addgene Blog FPbase: A new community-editable fluorescent protein database ReFigure: Save Scientific...technologies later. Organizations such as BenchSci and FPbase are working hard to enable researchers by making...BenchSci collates information about antibodies while FPbase curates information about fluorescent proteins ...

...TAG. Design your repair template with 55-100 base homology arms upstream and downstream of the insertion...using the ivTRT method, try to make the first two bases of the left homology arm guanine (i.e. GG); this... PMC3961003  Miura, Hiromi, et al. “CRISPR/Cas9-based generation of knockdown mice by intronic insertion...

...the 3rd day of CRISPR, Addgene gave to me: dCas9-based gene editing for cleavage-free genomic knock-in ...

...References Abril, U.N.O., et. al. (2022) A plasmid-based system for locating and containing escaped flies...

... broadens the conversation about genetics pgEd, based at the Department of Genetics of Harvard Medical...universities or cities have organizations (often volunteer-based) that put on public lectures, science cafés or science...

... delivery systems, especially lentiviral vector-based CRISPR pooled libraries that allow functional screening...barcoding library consists of 96 retroviral plasmids (based on the Murine Stem Cell Virus, MSCV) each with a...

...barcoded gRNA library is now ready for CombiGEM-based assembly into the CombiGEM lentiviral vector backbone...: PMC4420636. 7. Ma, Hongming, et al. "A CRISPR-based screen identifies genes essential for West-Nile-...