...Aging in a commentary about the new work published in Cell Metabolism. But, they note, there is plenty more... Skeletal muscle PGC-1α1 modulates kynurenine metabolism and mediates resilience to stress-induced depression...

...Joseph Campbell, and read about his "follow your bliss' mantra and never forgot it. Usually when I see ...you can.” - Eddy Page, Digital Media Production Specialist “My favorite career tip for landing a job is ...skills guide Resources on Addgene.org Find job listings at Addgene Get more career insight from Addgenies...

...sharing. Listen to learn more about Darrell, his research, and his focus on open science. Listen to the... Edward B. Page is a Digital Media Production Specialist at Addgene. He is the audio engineer and producer...

...sitting in the San Francisco International Airport listening to the Lion King soundtrack and writing this post...easier. Since the post doesn’t provide an exhaustive list of all the apps out there, we encourage you to mention...to learn how his company uses phage to test for Listeria in food products). I picked this post for a few...

...fact that every gRNA listed in the table has been used effectively in a published article. This blog post...your effective gRNAs, and we’ll add them to this list. Access the spreadsheet for submitting your gRNA...

...Milling, L. E., Derosia, N. M., Hoffman, Z. M., Gillis, J. E., Tjokrosurjo, Q., Markson, S. C., Huang,...Nguyen, T.H., Coxe, M.A., Miller, B.C., Yates, K.B., Gillis, J.E., Sen, D.R., Gaudiano, E.F., Al Abosy, R.,...., Trombley, J. D., Weiss, S. A., Brown, F. D., Gillis, J. E., Coxe, D. J., Doench, J. G., Haining, W....

...could help fight COVID-19. They’ve also collated a list of useful research resources.  "We realized that...only supports collaborations at Rutgers, but also lists data and experimental resources that are useful ...what caught our eye recently (not a comprehensive list). #coronavirus #SARSCoV2 #Covid19researchA thread...

... be a scientist) and they were part of a Twitter list with a scientific title (perceived by others to ...to be a scientist). Profiles and Twitter lists were searched for scientific titles generated from the United...dataset; 2) users not part of a scientific Twitter list were excluded from the dataset. 3. 60% of scientists...

...of the listed qualifications to apply for a position.  A job description is usually a wish list and the...your research skills to determine which job boards list positions interesting to you. Many job postings ...

... better to have a scientist up there, but the panelists were interesting enough to make up for the somewhat...Krzysztof Chylinski (who coincidentally speak the same Polish sub-dialect making their Skype communications extra...this extraordinary breakthrough. In fact, the panelists agreed that, for example, the technology is already...

...previously used reagent performs well, or you might publish a replication experiment that you conducted as ...good for you and good for science ReFigures are published instantly and are editable by the author at any...long before a study with multiple patients was published, Zika researcher Christa Osuna collated Zika case...

...biology toolbox organized, today’s post features a list of free online molecular biology tools all in one...applications Primer design tools Many of the above-listed plasmid mapping tools have built-in primer design...have a favorite online molecular biology tools not listed in today’s post? Share them in the comments below...

...-op to receive a degree. Northeastern compiles a list of hundreds of different jobs for students to apply...Addgene before. This meant I was responsible for establishing a new report and routine across the marketing...  Special thanks to my manager, Sr. Marketing Generalist, Steven; the Sr. Marketing Manager, Lynn, and...

...or leading. All three roles are important to accomplishing any goal or finishing any project. Doers are...effective manager, read the other posts in this series. Listen to our podcast interview with Connie Cepko to learn...characteristics of successful leaders. Below I've listed a few of my favorites. Additionally, you can find...

...discomfort appropriately to get the message across. Listen to our podcast interview with Harvard Medical School...give feedback in formal sessions using a specific list of questions after a task or period of work. Using...Addgene Blog to follow the entire series as it is published. For more reading try The Discipline of Teams ...

...community. In the long term, the research project establishes your professional brand. It offers colleagues...the way. Advice from principal investigators Tom Ellis highlights the importance of keeping up-to-date ...for you.   Acknowledgments Thank you to Dr. Tom Ellis (Imperial College London), Dr. Connie Cepko (Harvard...

...the fly. But just like the growing PubMed article list, the Genome Engineering Using CRISPR/Cas Systems...questions answered about CRISPRs? Check out the full list of 16 FAQs answered by Le Cong Read Addgene's CRISPR... Addgene Find protocols and gRNA design tools: List of CRISPR protocols developed by a variety of labs...

...lab, you'll likely find many of the antibiotics listed in the table below. Note, in this post we'll focus...filter unless otherwise specified. The above table lists some antibiotics commonly found in the lab, their...and E. coli strain are compatible! Check out this list of common E. coli genotypes and their natural resistances...

...could do something like puzzling or crafts, while listening to podcasts made for kids. Plan out meals and ... Micropublication will accept negative data or publish brief, novel findings. Most Micropublications contain...helpful to you no matter what you’re trying to accomplish during this period. ...

...being experimented on, too.) However, as research published on December 13 shows us, CRISPR/Cas9 techniques...Council. While the Nuffield Council has recently published a report on the social and ethical issues of genome...their research into both novel and previously established ethical issues raised around mitochondrial replacement...

...made to moderate and cross-check all data with published literature as soon as possible after submission... queries.  Looking for a bright, monomeric FP published in the last few years with an emission peak around...Fluorescent Protein Properties provides a master list of all proteins in the database, and the Interactive...

...sequenced There are several human disease models established in yeast, including a widely used Parkinson’s...the name of beer and bread. The following table lists some of the biological commonalities and differences...eukaryotic cells and peroxisomes are where methanol metabolism occurs in methylotrophic organisms. K. phaffii...

... using in an experiment, then spend the evening listen to some bloke at the pub telling me that actually... BSc in Anatomy, the University of Otago had established the Centre for Science communication and I was...discussions about the difference between airborne and aerosolised droplet transmission and how different fabrics...

...transform your work into a paper or a grant. Here’s a list of tools and resources that might help make writing...library or don’t have time to read the book, this list summarizes the key points of the book. The best ...each week, and there’s advice on how to actually establish that schedule. There’s also tips for setting writing...

...why the results you've measured should change established theory. So, what can you do to ensure that your...scientists need to communicate with non-scientists, to establish broader impacts for their work in education and... ask a friend who works in a different field to listen to you practice.  Ask them what they learned from...

... the example of our Antibody Guide, which was published alongside our new antibody services in the spring...was then assigned to our science communication specialist, Rachel, to write. Identify expertise While ...final review to catch those pesky typos, and hit publish. Making content is an iterative process. We are...

...protein (HRP), primarily used in Western blots and ELISAs, cannot be stored in sodium azide at all, as it...of signaling molecules. Assay Type Westerns and ELISAs mostly use HRP, a chemiluminescent conjugate that...there’s a specific reason you can’t use it for your ELISA and Western, I’d highly recommend sticking with ...

....  Review the conference schedule and attendance list. Plan the sessions you will attend. Research the...meal is a great way to build relationships. Make a list of experimental problems or questions you are pondering...Congratulate your connections when they get a new paper published. Stay in touch enough to plan to meet up at the...

...Albert Einstein College of Medicine has recently published techniques to follow translation in living single...presented a novel technology, back then still unpublished, that allowed the visualization of specific de...combination of FUNCAT and PLA PLA was originally published by the Söderberg lab in Sweden as a method for...

...Note: After this blog was published, a bioRxiv preprint that questions the conclusion of inter-homologue...with a storm of media coverage. But the paper published August 2nd in Nature gives us even more to talk...Since the embryos produced were not being used to establish a pregnancy, the researchers were able to examine...

...CytERM with mTurquoise2 and dTomato, which are established monomeric and dimerizing FPs respectively, are...compared with mEGFP or mTurquoise2, which are established true monomeric fluorescent proteins. Concluding... experience that is gathered can be shared by publishing it as (part of) figure 1, uploading it as a preprint...

...producing the fluorescent protein of interest under realistic conditions, i.e. with low excitation power. By...power experiments. Measuring photostability at realistic powers will provide better insight into the photostability...photostability in living cells provides a much more realistic view of photostability. Selecting a photostable...

...’re on a profile page. You might be looking at a list of suggested connections and you’ll see the boxes...It’s also a great way to keep track of past accomplishments. Follow all the rules of good resume writing...writing. Include examples of concrete accomplishments in both the technical and “plus skills” areas. Include...

...lends itself well to assays like western blots and ELISAs. Chromogenic reactions produce a color change, ...immunohistochemistry, though they are also a common choice for ELISAs.  Biotin Biotin is used in a range of antibody-...BioRender.com.     Many biologists can lead perfectly blissful, productive lives without thinking about these...

...purpose. Tang wondered if he could use previously established mouse lines expressing GFP in specific cell types... using GFP as a scaffold suddenly seemed very realistic. Envisioning GFP as a substrate promoting dimerization...optogenetics experiments in specific cell populations. Listen to Our Podcast Interview with Connie Cepko Combining...

...contributed by Johnna Roose. This post was originally published on Johnna's New Under The Sun Blog and is part...you will likely have is sifting through the long list of titles for something that is actually useful ...Manuscripts by Johnna Roose Dos & Don'ts When Publishing a Scientific Manuscript by Maaike Pols, PhD, ...

...audience’s eye and draw people in. That can be accomplished with an exciting title and attractive figures...in less than a couple of minutes. How does one accomplish all of that? What follows are some tips to help...logical progression of your work, and you can accomplish this by employing a neat and organized format...

...Originally published Apr 30, 2020 and last updated Jul 12, 2021. What started out as a small collection...lab instead of a BSL-3 lab. Jesse Bloom’s lab published a protocol and reagents for pseudotyping lentivirus...Travers M, Chen Y, Garcia N, Whiteman MB, McKay LGA, Lelis FJN, Habibi S, Cai Y, Rennick LJ, Duprex WP, McCarthy...

...has developed a new, plasmid-based biosensor to visualise changes in histone methylation in living cells...substrate (the histone-derived peptide) which can localise the construct specifically to the histone protein...in the middle of a FRET pair, which is used to visualise the results. When methylation occurs at the histone-derived...

...more likely to view your proposals favorably. 5) Listen to your supervisor’s viewpoint and brainstorm about...with your supervisor, prioritize your action item list and set some approximate timelines. Be sure that...conflicts if you communicate your ideas assertively, listen to his or her viewpoint, and discuss options for...