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We narrowed to 573 results for: BASE

Showing: 301 - 350 of 573 results

  1. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    Published
    April 18, 2024, 1:15 p.m.
    ... different categories based on function: knockout (KO), activate, inhibit, base edit, and other. The first...

  2. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    Published
    Aug. 10, 2021, 1:15 p.m.
    ...point where the hydrogen bonds break between the base pairs. This results in the separation of the two...using PCR. qPCR requires the addition of a probe based fluorescent dye that intercalates with any dsDNA...

  3. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ... scanning By Seth Kasowitz As multiple antibody-based vaccines are deployed around the world to combat...deactivate photocleavable gRNAs within seconds. A CRISPR-based lineage tracing tool captures rare clones that represent...

  5. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    Published
    Feb. 23, 2016, 3:30 p.m.
    ... question to ask is how the efficiency of PITCh-based genomic insertion would compare to CRISPR sticky-end...Mathieu, et al. (2015). “A Scalable Genome-Editing Based Approach for Mapping Multiprotein Complexes in Human...

  6. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    Published
    May 15, 2014, 3:33 p.m.
    ...and fluorescence. Biosensors: A wide array of GFP-based fluorescent biosensors has been designed to detect...separates mixtures of cells into distinct populations based on fluorescent signal. Thus, FACS can be used to...

  9. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...less photostable than GFP. In addition, antibody-based amplification of their signals is weak and increases...specific antibody binding sites and primary antibody-based detection, smFPs create much brighter signals for...

  10. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    Published
    July 25, 2019, 12:03 p.m.
    ...different ideas. We are a huge fan of the Golden Gate based MoClo system. The vectors mentioned in this blog..."The FAST technique: a simplified Agrobacterium-based transformation method for transient gene expression...

  11. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    Published
    July 9, 2019, 12:52 p.m.
    ...money. They also usually have a good event-planning base and will know how to reserve space, time events,...I think many of the students learned something. Based on the initial guidelines I received from the teachers...

  12. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    Published
    May 30, 2019, 12:44 p.m.
    ...allowing for in vivo biotinylation through a plasmid-based biotin ligase. Biotinylated dCas9, with bound DNA...genomic region is another example of the many tools based on targeting of a genomic locus through a specific...

  13. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    Published
    Jan. 17, 2019, 1:38 p.m.
    ...arises here. Notwithstanding access restrictions based on permissions, however, in some cases it would ...provides the RSpace electronic lab notebook. Rory is based in Cambridge, Massachusetts, and feels privileged...

  14. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    Published
    Dec. 7, 2020, 2:53 p.m.
    ...genome and bind target DNA sequences using RNA-DNA base pairing. Separately, TnsABC forms a transposase ...tools such as Cas9-recombineering, CRISPRa/i, and base editing. Leveraging a unique marriage between distinct...

  15. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    Published
    July 16, 2020, 1:15 p.m.
    ...demand model with weekend and holiday effects. Based on these observations, we considered the following...enable next-generation therapeutics. Prime editing, base editing, PACE, and DNA-templated synthesis are four...

  16. Great Results Start with Great Standard Curves

    Type
    Blog Post
    Published
    Aug. 15, 2024, 1:15 p.m.
    ...extrapolate the concentration of the unknown sample based on its measured response. An example of this is ..., the more accurate the curve. Though it varies based on the assay, the ideal %CV is less than 15%. You...

  17. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...Addgene blog Introduction to Antibodies Plasmid-based Recombinant Antibodies Polyclonal Antibodies Resources... Shokri A, Chotteau V. Antibody capture process based on magnetic beads from very high cell density suspension...

  18. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    Published
    May 21, 2024, 1:30 p.m.
    ... Other fields, often highly technical or skills-based ones, may not use them very often. Similarly, entry-level...or sharing relocation plans. They can be skills-based: if you're switching fields, you can use your cover...

  20. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ...consistent development and improvement of CRISPR-based technologies over the past decade. CRISPR screens... fact supported by the wide array of lentiviral-based CRISPR libraries available at Addgene. The large...

  21. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...determined using a chromogenic endotoxin detection assay based on the amebocyte lysate method. Purity Purity of...Schematic of the barcode matching system and PCR-based confirmation of transfer plasmids used in Addgene...

  22. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ...additionally allow for visual selection of transformants based on expression of RFP in the seed (Emami, Yee and...inflorescence (the flowering portion of the plant) near the base of the stem. This buys you a few days until new ...

  23. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ...1990s, researchers developed viral vector systems based on retroviruses like Moloney murine leukemia virus...actively dividing cells. Another type of vector, based on adenovirus, could infect non-dividing cells, ...

  24. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    Published
    Nov. 5, 2014, 3:41 p.m.
    ...conformational changes, cleavage activity using FRET-based biosensors, and interactions between DNA and proteins...limited to a single measurement. This method is based on the fact that the donor is quenched when FRET...

  26. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    Published
    April 3, 2014, 8:05 p.m.
    ...response elements). Promoters are about 100 to 1000 base pairs long and found upstream of their target genes...Promoter specificity Aside from choosing a promoter based on type of RNA transcript, you will also need to...

  28. Editor's Choice, July 2016

    Type
    Blog Post
    Published
    Aug. 5, 2016, 3 p.m.
    ...Tool for Gene Expression in Mammals Recombinase-based State Machines Enable Order-dependent Logic in vivo...

  31. Plasmids 101: How to Name Your Plasmid in 3 Easy Steps

    Type
    Blog Post
    Published
    Feb. 13, 2014, 1 p.m.
    ...details. Once you know the backbone a plasmid is based on, you can usually derive: a) the bacterial antibiotic...commercially available empty backbones, visit Vector Database. Step 2: Insert name  Include information about...

  32. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    Published
    June 28, 2018, 3:01 p.m.
    ...cells. A major bottleneck, however, to these IP-based assays is to find (or generate in-house) a pre-validated...develop a hassle-free, highly efficient Cas9 protein-based method for epitope tagging in mammalian stem cells...will have to design your crRNAs using other web-based design tools (for C-terminus tagging, pick a crRNA...

  33. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ... PAM requirements and editing abilities, such as base editing, nicking, cleaving, etc. The possibilities...toolkit to facilitate other lentivirus- and AAV-based studies. Unified MoClo Systems While many MoClo ...acssynbio.9b00322. Kim, Matthew S., et al. Degron-Based bioPROTACs for Controlling Signaling in CAR T Cells...

  36. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ...to understand. Classes are based on the main Cas effector; types are based on main function and composition...composition; subtypes and variants are typically based on species of origin. There are currently two classes...

  37. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    Published
    April 19, 2018, 1:25 p.m.
    ...a quick chat can be rather tricky (think people based in different countries). Here social media is a ...References 1. Sinche, Melanie, et al. "An evidence-based evaluation of transferrable skills and job satisfaction...

  39. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    Published
    Nov. 19, 2020, 2:15 p.m.
    ...results (Sinnett et al., 2017; Powers et al., 2019). Based on the work of the consortium, two gene therapy ...guide RNA and a hyperactive version of the human RNA base editing enzyme (“Editase”), ADAR2, into the hippocampus...iPSCs, MECP2 animal models, tissue banks, Rett databases, and other helpful tools.  References: Amir ...

  40. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    Published
    June 20, 2024, 1:15 p.m.
    ...regulators. The genome of E. coli is about 4.6 million base pairs long and contains a single origin of replication...into a “pseudoknot” held together by intramolecular base pairs for the protein to be translated. An antisense...

  41. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ...lab has developed a multiplexing approach to Cas9-based pooled CRISPR screens called CROPseq-multi (Walton...immunogenicity typically associated with viral vector-based therapies. REVeRT supports advanced gene expression...negative-going sensors (with fluorescent signal at baseline voltage), the investigators found that administering...

  42. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...point on the axis determined by the researcher, based on their controls. You can usually tell where this...Addgene blog Conventional vs. Flow Cytometry Plasmid-based Recombinant Monoclonal Antibodies Introduction to...

  43. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    Published
    Oct. 19, 2017, 1:54 p.m.
    ...nucleotide level but share an overall structure of 13 base pair palindromic repeats separated by an 8 bp asymmetric...2010. “An Improved FLP Deleter Mouse in C57Bl/6 Based on FLPo Recombinase.” Genesis 48(8): 512–20. PubMed...

  44. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    Published
    Nov. 25, 2015, 4 p.m.
    ...environment. The “how many?” question lies at the base of all these questions as there is not particularly...for their buck - make them demand more services based on what they're spending. Increase the number of...

  45. 500,000 Plasmids Shipped and Counting!

    Type
    Blog Post
    Published
    March 26, 2015, 3:24 p.m.
    ...team creates stabs for each plasmid being shipped based on the information in our lab software, labeling...

  46. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    Published
    March 13, 2014, 4:08 p.m.
    ...off-target effect. The double nickase system is based on the Cas9 D10A nickase described in Figure 4 of...express without the G. Because the G is only one base, the Zhang lab usually adds it when they order the...oligo from IDT (non-PAGE purified oligo is fine based on new data). For general transfection, you could...

  50. Let There Be LITE Plasmids

    Type
    Blog Post
    Published
    Nov. 21, 2013, 3:56 p.m.
    ...MIT, the use of customizable DNA-binding domains based on transcription-activator-like effectors (TALEs...
Showing: 301 - 350 of 573 results