...organization on the planet, in which case whoa! Mission accomplished! One example of a mission-based team in academia...are currently about 5.5 million articles in the English version of Wikipedia alone. Humans have been forced...snapped out of the phase of my life that had me listening to Simon & Garfunkel’s “I Am A Rock” on repeat...

...And you’ll be able to find all published videos in our Quick Tips playlist:  Want your quick tips even ...

...names and we’ve recently chosen four names as finalists. Now, we’re asking you to help us make the decision...submitted by more than one person, we selected the finalist based on the first time of submission.)     ...

...use of pMD2.G obtained from Addgene. Skimming the list, one gets a sense of the broad usefulness of pMD2...CRISPR, and so much more (find the full, searchable list here). Tips for using pMD2.G At Addgene, we use ...

...new forms of science communication. Click here to listen to the interview with Tom McFadden!   1. Rosalind... Edward B. Page is a Digital Media Production Specialist at Addgene. He is the audio engineer and producer...

...concrete advice on how to pick a lab and a project. Listen to the interview here! Podcast Breakdown (Time ... Edward B. Page is a Digital Media Production Specialist at Addgene. He is the audio engineer and producer...

...connections to mentorship and networking opportunities, publishing assistance, and more to Instrumental Access participants... about Global Plasmid Distribution Check out the List of Institutions that Deposit their Plasmids with...

...A newly established all-in-one vector construction system for CRISPR/Cas9-mediated multiplex genome engineering.... “However, there had been no systematically established way of making an all-in-one vector for multiplex...

...research infrastructure that has taken decades to establish.These funding decisions will have far-reaching consequences...difficult to maintain or access. These services can be listed as direct costs from a CRO in grant applications...

...blot, immunohistochemistry, immunocytochemistry, ELISA — but in all cases, it contains a detailed description...completeness. The information is then formatted and published as a report with a DOI for citation. If you’re...

... One obvious way to do that is and has been to publish in journals with a high “impact factor,” a measure...to say all of us here would add materials to that list (plasmids, yay!). Scientists are blogging, talking...

... This blog was originally published on BitesizeBio here. As part of my job ensuring plasmid quality at...Acknowledgements: Thank you BitesizeBio for originally publishing this and allowing us to share it with our readers...

...and their respective researchers. Check out the lists below for more details. Benefits to the provider...applications on modifications or inventions Right to publish without editorial comment or review by the Provider...

...cells but extends to freely moving animals, as established for conventional optogenetic tools." In total...applied the LITE system to 28 different genes to establish it as “a novel mode of optogenetic control of ...

... the Whitehead Institute and MIT, started with a list of 17 signaling pathways that are frequently implicated...each of these pathways. Rather, they relied on published studies in the literature to curate the perfect...

...good lists of questions on the internet. To make the most of the opportunity, use a written list and take...

...Since the first research applications of GFP were published in the 1990s, biologists have spent a lot of time.... Subach OM, Patterson GH, Ting LM, Wang Y, Condeelis JS, Verkhusha VV. A photoswitchable orange-to-...

...microbial DNA from groundwater. Now, they’ve recently published data demonstrating that CasX is active in both...for cancer research The Sidi Chen lab recently published two CRISPR knockout libraries for cancer research...

...robust M24 promoter. Originally isolated from the Mirabilis mosaic virus, the constitutively active M24 promoter...this new vector, pSiM24, can also be used with biolistic methods, making it useful for engineering plants...

...irreversible protein-peptide interactions. In a study published in Proceedings of the National Academy of Sciences...Proceedings of the National Academy of Sciences paper [published online on March 17] should cover it. Addgene: ...

... cyan, blue, and orange spectra. A comprehensive list of cell markers all tagged with mEmerald. Plasmids...variants. The mEmerald plasmids offer a comprehensive list of readily available targets since the Davidson ...

...should be a max of 500 bp (preferably). I compiled a list of three options from the Addgene catalog. These...osa-miR528. My manager, Katherine N., reviewed the list and followed up with her manager. They looked at...

...bench, look for “technical or product support specialist,” “field application scientist,” or “scientific...own research project.  3. Do you prefer being a generalist? Working on a variety of projects and in different...

...sequence information contributed by the depositor, published backbone sequences, and any known sequences for...Addgene. By using Snapgene’s “Import primers from a list” function, we can detect all of the primers we have...

...information.” Addgene is here to help scientists accomplish their goals. Many nonprofits must spend a lot...Seeding Labs Addgene Supports Rare Disease Research Listen to Our Interview with Addgene Board Member, Connie...

...more blue flame plasmids, with more added to the list weekly. The Blue Flame Award, first distributed ...Deposit Find Additional Hot Plasmids Find the Full List of Blue Flame Depositors ...

...awesome. The process of writing, editing, and publishing blog posts runs its course more quickly than ...supervisors warned me, the swift gratification of publishing posts can be addicting. Even more satisfying,...

...”. I have added a few comments of my own to this list. Need feedback on their work but prefer to be approached...Addgene Blog to follow the entire series as it is published. Good introductory reference books on managing...

...backbones that have been designed, tested, and published by academic scientists. To help you find the vector...Molecular Cloning Guide & Other Plasmid Protocols List of common epitope tags ...

...are interested in breeding dogs who also enjoy listening to podcasts A series of Tweets breaking down...likely to closely read an email sent out to a large listserv or one sent to you and a few other people on your...

...Bandrowski et al., 2016). It’s also been well established that recombinant antibodies are excellent for...Bechtold H, Dumitras C, Gong B, Nguyen K, van der List D, Murray KD, Engebrecht J, Trimmer JS (2019) A ...

...video of her singing about the paper recently published by her lab and Mustafa Khammash's lab at ETH Zürich...UriAlonWeizmann, here my song about our paper recently published in @nchembio Title: Bye bye L-arabinose drive....

... need, and most of us could use more mentors to listen, guide and support us during training and throughout...your head.  Here are some sites that have compiled lists of grants: Science: Where to Search for Funding...

...routine plasmid propagation, with complete ITR loss abolishing AAV packaging. To prevent ITR loss, it’s best...://doi.org/10.3389/fnana.2019.00093 Wilmott P, Lisowski L, Alexander IE, Logan GJ (2019) A User’s Guide...

...several years ago we partnered with Seqwell to establish a next-generation sequencing (NGS) QC platform...serotype of our AAV preparations. We recently published a description of the VGS process and use cases...

...the diverse options in nonprofit science. No one list can ever encompass them all. We can’t designate ... like you What is different about academia? Publishing in peer reviewed journals is absolutely necessary...

...for being a part of our community, we’ve curated a list of our 10 top-viewed posts from 2015 and encourage...of our career posts has made it to the top of the list this year. While we love academic research, we hope...

...cellular behaviors, such as differentiation or metabolism. Our attempts to manipulate these cellular behaviors...promise to be host-independent, beyond the oft published metabolic engineering realm of S. cerevisiae and...

...sensitivities can now be applied to dozens of other published FRET-based sensors. For example, his team is now... improve upon an indicator they developed and published in 2010 for visualizing the transcription factor...

...using the power of light. In a recent article published in Nature Methods, Boyden and his team describe...can figure out what they are necessary for. We published our first paper on optogenetic tools for neuroscience...

...researchers are open to sharing knowledge and establishing collaborations, so it doesn’t hurt to reach ...will need, any required training, and a priority list of experiments you hope to complete. Share this ...

...FLiPs) Fluorescent proteins can be applied in a long list of creative ways. In her graduate work in Michael... lab at Stanford, Xin Zhou greatly expanded that list by using photoswitchable fluorescent proteins to...

...enzymes, the app is quite helpful and provides a list of directions including which buffers to use and... I have found myself wishing that it had a codon list for each amino acid, but, overall AMINO can be beneficial...

...further introduce him to the Addgene community. Listen to the interview here! Doench majored in history... Edward B. Page is a Digital Media Production Specialist at Addgene. He is the audio engineer and producer...

...available. With new fluorescent proteins being published every month, deciding on the best protein for ...common use (particularly not the most recently published ones), they can be a good starting point. In many...

...the mid-aughts, the first yeast GCE paper was published, followed soon by GCE done in mammalian cells ...community to fulfill their mission. While they’ve shortlisted several protocols, including their phosphoserine...

...infect both non-dividing and dividing cells. They accomplish this through nuclear localization signals which...on the Addgene Blog Learn More about Lentivirus Listen to Connie Cepko Discuss Gene Therapy with AAV Figure...

...monoclonal, polyclonal, scFvs, nanobodies, and the list goes on. But have you heard of an antibody isotype...cytometry, immunohistochemistry, immunocytochemistry, ELISAs, and Western blotting.  The five isotypes of antibodies...

...Finally, as previously mentioned, enlist the help of your network to polish the final draft prior to submission...

... from this collection in your experiments and published your data, please fill out this form and we’ll...complete QC and you can find all available plasmids listed on Ginkgo’s collection page.  Bacterial expression...