...modulators regulate transcription and chimeric efforts make a precise edit or insertion via a chemical modifier...but it certainly suggests that Addgene depositors make CRISPR tools that fill needs in the community. The...centers around the impact of science, but our data makes it clear that it’s also a story of community, cooperation...

...about using non-homologous end-joining (NHEJ) to make deletions or homology-directed repair (HDR) to create...very small homology regions (5-25 bp) for repair, making it easier to construct targeting vectors. Addgene...downregulated, MMEJ represents another method for making targeted modifications. A recent publication by...

... does making transgenic organisms with Cas9 risk creating a gene drive? The simplest way to make a gene...Institute and Harvard Medical School. Scientists making transgenic organisms with Cas9 should be aware ...

...culture experience. Let’s go over the basics of making rAbs. Production Recombinant antibody production... because it secretes fewer of its own proteins, making the purification process simpler. Due to its high...Maity et al., 2015). If your antibody is not IgG, make sure to look up the setting for your isotype.  Antibody...

...researchers need to reflect upon before they attempt to make essentialist connections between biology and ancestral...Map (DepMap), a cancer dependency database used to make this information accessible to the research community... at all. This unexpected discovery, which didn’t make sense in any kind of biological framework, suggested...

...below). Addgene scientists inspect each sample and make sure they have been barcoded with a unique ID number...therefore particular sample is missing so that we can make a new tube from a replicate sample. The transformation...replicate and retain the plasmid. This allows us to make unlimited copies of each plasmid so that we never...

...You will often hear the term ‘grant writer’, but make no mistake, researching, winning, and effectively...writing. In fact, many grant applications and grant makers have moved away from long written proposal narratives...relationships with foundations and government grantmakers. We keep up-to-date on the changing guidelines...

...amazing 111 countries (Figure 1)! But while we were making the materials available to many countries, import... to increase accessibility to our repository by making it easier to find materials and learn about them... know we will continue to ask “What can we do to make our materials both available and accessible to scientists...

...truncated forms that limit its functionality. To make biophysical and structural studies more feasible...simplicity and efficiency of this dual-plasmid approach makes it a valuable resource for advancing our understanding...assembly of the split luciferase is reversible, making PILATeS suitable for live imaging with a time resolution...

...organizers in their town hall.  Iterative improvement - Making Binning Singletons better Our experience running...also helped us find areas for improvement. We will make a greater effort in the future to match people within...Binning Singletons is its own organization, working to make scientific meetings more productive for attendees...

...and many of my scientist colleagues have as well. Making the transition from the bench can be jarring for...aren’t a lot of resources out there for scientists making this transition. In this installment of the Addgene...come up with a list of things to consider as you make this transition. Of course, a “desk job” could entail...

... bench. Specifically, roles in technical support make excellent use of the skills developed by life science...advantageous to speak multiple languages - that makes communication with customers from around the world...convenient alternative for asking a quick question or making initial contact, but is less suitable for troubleshooting...

...well-researched field, you may need to get specific to make sure your article doesn’t turn into a textbook. ... short bursts (even twenty minutes was enough to make some progress). For most researchers, it’s not always... into something cohesive-- change the sentences, make it flow, and start telling the story. Like any editing...

...zebrafish embryo develops outside of the mother, making them ideal for studying vertebrate embryogenesis...zebrafish To highlight some of the features that make zebrafish useful, I’ll outline two recent lineage...unprecedented views into cognitive processes like decision making (Kim et al, 2017). “Brainbow” fish lines have been...

...specific target, and the molecular processes that make aptamer biology a powerful tool. In this post, we...dependency on magnesium and higher thermal stability, making it a potentially more useful tool for in vivo studies...interaction between Corn and its small molecule makes Corn more photostable than Spinach and Broccoli,...

...false positives observed in the cloning process, making it easier to produce the constructs you need. Expression...affecting its translation initiation and instead makes initiation heavily dependent upon the core of a ...to simplify the protein purification process by making it easy for a researcher to quickly generate N-...

...target cells with Cas9. Hooray! Now it’s time to make sure your genome edits went according to plan. In...sequence was inserted or deleted accordingly. Pro tip: make sure your PCR amplicon isn’t too large so that you...issue, you may need to select an alternative gRNA. Make sure to check out our validated gRNAs to avoid rolling...

...Being smaller means scFvs are easier and cheaper to make because they can be expressed in bacteria, while...with other protein domains, much like lego pieces, makes them better suited than antibodies to be used as...T cell receptors and are expressed on T cells to make CAR T cells. The extracellular scFv portion of the...

...sometimes it can be a big challenge. But ask any filmmaker or videographer and they’ll tell you, a lot of...Design-Build-Test-Learn cycle adapted for video making.   Cycle One: The Ironing Board Control Center...: There were still a few adjustments I wanted to make to ensure smooth filming in the future, but we were...

...commitment. Another advantage is that this process makes it easy to generate wild type and mutant constructs...content, contains a lot of repeats--any of which could make the PCR step difficult--OR there is no convenient...rather than using a restriction enzyme or PCR to make linearized vector, the Cas9 enzyme along with a ...

...Becker muscular dystrophy (BMD). Dystrophin’s size makes it difficult to deliver via gene therapy, so researchers...normal function. Even low-frequency editing could make a huge difference in DMD, and it’s estimated that...Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang F. In vivo genome...

...2008). It can also cross the blood-brain barrier, making it useful for applications targeting the CNS (Issa...expression (Tervo et al., 2016). This functionality makes it useful for mapping neural connections. Although...depends on your experimental system. As we cannot make universal serotype recommendations, the following...

...a given phage will recognize on a host cell. To make matters even more interesting, much of what a phage...display little to no homology to anything else, making it difficult to quickly identify possible gene ...the T7 polymerase and a few proteases knocked out making them particularly useful for protein production...

...they are relatively small and easy to work with, making them the go-to choice for studying mammalian biology.... For editing the genome, the CRISPR/Cas system makes use of 3 components, a guide RNA (gRNA) of about...content in the mouse genome and the Cas9 enzyme will make a cut at the target site. This is when it gets exciting...

...characterized by large DNA recognition sites, which makes them highly specific.  Jurassic Park by Michael ...   Star Trek: Into Darkness (2013) which is the remake of the space-melodrama Star Trek II: The Wrath ...has always served as a vision of the future and to make us reframe our perspective of the current. Although...

...our lab so we opted for AAV. While these benefits make AAVs useful for a wide variety of experiments, care...can still reduce AAV titer. For long term storage, make > 25 μl aliquots in 0.5 ml tubes, snap freeze in...humans and additional improvements are being made to make them even more useful for therapeutic purposes. ...

...ve been planning, it is a good idea to pause and make sure that your antibody has been appropriately validated...guarantee recognition of the target antigen. You want to make sure that your antibody binds to what you expect... epitope information for some antibodies, which makes it hard to ensure that you have unique antibodies...

...with DIO/FLEx vectors and off-target expression making it easier to determine the spread pattern around...your cells of interest. You should now be able to make smaller adjustments to a set of injections into ...K, Sanders E, Tasissa M, Kostman M, Tillgren M, Makana Hanley L, Mueller I, Mitsopoulos A, Fan M (2020...

...characteristics to consider when selecting a dye or ligand to make your selection easier, as well as highlight some... imaging tools in biological research. This does make finding the right dye for your experiment more complicated..., M., Cincotti, G., Rampioni, G., Visca, P., & Kolmakov, K. (2023). Expanding the microbiologist toolbox...

...enjoying the party. There are so many teams that make Addgene functional, handling everything from facilities...this diverse group of talented individuals that makes Addgene — well, Addgene. We're so grateful for each...

...that we’ve learned in the lab that will hopefully make your life a little easier. These can range from ...Putting on lab gloves can sometimes be annoying. To make it easier, try this quick trick. Just flip, and ...

...get to talk to scientists from around the world making breakthrough discoveries!” I knew right then I ...way that a connected and collaborative community makes not just for a better day at the office but for ...

...samples from my assay at like 23:00, so I decided to make a giant snow angel for biologists in our building...performing routine lab maintenance tasks. Hot tip: make sure your PI/facilities are okay with the decorations...

... Other Retinopathies 14:39-25:59 : Strategies to Make Gene Therapies More General (more than correcting...Success 40:55-end : Professor Cepko's Thoughts on Making Scientific Information Accessible We'd like to...

...Expressing these chromoproteins in E. coli would make it easy to distinguish colonies from one another...always get a few isolated colonies. I should probably make it a bit more challenging, potentially by using ...

...wanted to take a week to celebrate the scientists who make a big impact in open science. Depositors consistently...this year, Addgene depositors quickly stepped up to make COVID-19 reagents available to researchers across...

...sometimes be elusive or have a certain mystique that makes them difficult to approach for the youngster just...people to do science? 16:08 - 22:04 : How have the make-up and scope of your graduate projects changed throughout...

...there had been no systematically established way of making an all-in-one vector for multiplex genome engineering...with a broader array of Cas9s, which they will also make available when they are validated and ready. “Currently...

...providing quality control and customer support, making plasmids easy for you to find and request in our... plasmids as of March 26, 2015. How does Addgene make it happen? Our awesome team of Addgenies! Our team...

...only viral protein present on the virion surface – making it critical for viral attachment and fusion. Once...  The EBOV glycoprotein’s role in infection also makes it a critical component of vaccines and the target...

...changes to our website! Part of sharing science and making access to materials easier is improving the tools...may be less obvious — but every change is meant to make your experience on our website better so you can...

...amoebasisters.tumblr.com/post/139944465237/lactose-around-we-can-make-some-enzymes-for   The take home message was that... that being creative and telling a story makes scientific topics both interesting and approachable!   ...

...able to communicate effectively is part of what makes working in a team productive. Take all the opportunities...or want to start a peer mentoring group? "What Makes a Good Mentor?" Form Your Own Peer Mentoring Group...

... by the Provider Limited liability Addgene makes it easy Even with all those benefits, we know that...auto-approve MTAs. Whatever your preference, we are here to make it fast and easy. Fast Approval: The UBMTA used...

...in the process. “We had several motivations for making GreenGate available through Addgene,” Lohmann said...system, the more building blocks will be available, making it easier for us to do our work.” References 1...

...Konermann said she is already aware of research groups making use of the new optogenetic tools in C. elegans ...neurons, she offers a tip: save yourself some time by making an AAV1 supernatant. (The details can be found ...

... your obscure growth medium, these apps can help make your lab life a bit easier. Since the post doesn...apps you’ve found useful in the comments section to make this post even more useful to your colleagues. Technique...

...eagerness to get, as well as give, information to make improvements. Members connect directly with one...card, afternoon off, etc.) Act on information to make change whenever possible. Be public about changes...

...gRNAs can save you the time that would be spent making and testing entirely new gRNA designs. You can ...How to Design Your gRNA Use the CRISPR Software Matchmaker Browse Alll of Our CRISPR Posts Additional Resources...

...and her lab members have noticed that the meetings make the lab run more smoothly. With a concrete plan ...Seeking Feedback Management for Scientists: What Makes a Good Manager Anyway Developing Transferable Skills...