We narrowed to 481 results for: mme

1. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

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   Blog Post

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   Oct. 20, 2020, 1:15 p.m.

   ...that you've been working on, let us know in the comments below! Additional resources on the Addgene blog...

2. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

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   Blog Post

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   Oct. 10, 2019, 12:41 p.m.

   ...Limitations with GFP in imaging  While GFP has been an immensely powerful tool to study proteins in vivo, it does...

3. The Golden Stopwatch Award: Recognizing Facilitators of Research

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   Blog Post

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   Sept. 5, 2019, 1:06 p.m.

   ..., office of knowledge transfer, office of commercialization Additional resources on Addgene.org Addgene...

4. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

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   Blog Post

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   April 25, 2019, 3:01 p.m.

   ... and named the protein mTFP1. The researchers recommend using mTFP1 with a 445/30 nm excitation filter...

5. New and Upcoming Viral Vectors - Spring 2019

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   Blog Post

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   March 26, 2019, 1:40 p.m.

   ... (pAAV.hSyn-FLEX.iGABASnFR.F102G) is noticeably dimmer than iGABASnFR, and accumulates in the endoplasmic...

6. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

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   Blog Post

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   Jan. 24, 2019, 2:24 p.m.

   ...large brain samples. For large brain samples, we recommend pretreatment with ScaleCUBIC1 (Susaki et al., ...

7. Arabidopsis in Education: How the Arabidopsis Biological Resource Center Brings Plant Science to Life

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   Blog Post

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   April 12, 2018, 1:02 p.m.

   ... in ordering from ABRC for the first time, we recommend reaching out to us at [email protected] for help...

8. In Vivo Biotinylation of Bacterial Fusion Proteins

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   Blog Post

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   Jan. 25, 2018, 2:09 p.m.

   ...mouse (NEB, E8032S) antibodies against MBP are commercially available. Factor Xa loves calcium, but metal-chelate...

9. Identifying Sequence Elements with SnapGene's Feature Database

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   Blog Post

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   Dec. 21, 2017, 2:06 p.m.

   ... annotated boundaries for control elements in commercial plasmids were inconsistent and sometimes clearly...

10. The Strength of Story Telling

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    Blog Post

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    Dec. 19, 2017, 2 p.m.

    ...relationships are all things almost all of us are programmed to appreciate and understand. We build narratives...

11. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

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    Blog Post

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    Sept. 21, 2023, 1:15 p.m.

    ...model system is a mouse, given the wealth of commercially available mouse monoclonal antibodies. The good...

12. Antibodies 101: Epitope Tags

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    Blog Post

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    May 11, 2023, 1:15 p.m.

    ...from molecular and biochemical fundamentals to commercial systems. Appl Microbiol Biotechnol. (2003) 60...

13. Antibodies 101: Chimeric Antibodies

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    Blog Post

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    April 25, 2023, 1:15 p.m.

    ...anti-Protein B antibody (Fig. 3)    Figure 3: Recommended secondary antibodies for rat IgG1 and mouse 1gG2a...

14. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

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    Blog Post

    Published

    June 9, 2016, 2:30 p.m.

    ...using NgAgo? Let us know how it’s working in the comments section! Read About NgAgo User's First Impressions...

15. Plasmid Cloning by PCR

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    Blog Post

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    March 29, 2016, 2:30 p.m.

    ...piece (see NEB for more information). Thus, we recommend that you add 3-6 bases upstream of your restriction...

16. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

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    Blog Post

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    July 7, 2015, 3:36 p.m.

    ...In the summer of 2013, a remarkable nine papers describing CRISPR/Cas9 genome engineering methods for...

17. Advice for Moving Into Sales After Your Science Postdoc

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    Blog Post

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    Aug. 21, 2014, 3:15 p.m.

    ...interest that would be able to apply themselves immediately to the position. In case you are interested yourself...

18. Mentoring for Scientists: I Have a Mentor, What Now?

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    Blog Post

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    March 6, 2014, 7:12 p.m.

    .... Meet 10 PhDs with interesting careers, find a summer internship, etc.). It is helpful to work together...

19. Extracurricular Activities for a Strong Science Career Path

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    Blog Post

    Published

    Jan. 21, 2014, 3:08 p.m.

    ...more about next. Leave your suggestions in the comments section.  ...

20. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

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    Blog Post

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    July 21, 2020, 1:15 p.m.

    ... a DNA ladder with known concentrations. Many commercial DNA ladders will tell you the concentration of...

21. 25 Work From Home Tips for Scientists

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    Blog Post

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    March 19, 2020, 1:15 p.m.

    ...send a wrong message for some people. We should recommend #PhysicalDistancing. Social interactions and #...

22. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

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    Blog Post

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    April 7, 2022, 1:15 p.m.

    ...including a terminal PDZ ligand. The researchers recommend using the SGZ for in-culture work only, GPI anchors...

23. Celebrate National Book Month with Addgene

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    Blog Post

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    Oct. 3, 2019, 12:47 p.m.

    ...community know what your favorite book is in the comments! Additional resources on the Addgene blog Learn...

24. New and Upcoming Viral Vectors - September 2019

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    Blog Post

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    Sept. 12, 2019, 1 p.m.

    ...! Control AAV For many AAV experiments, we recommend validating the serotype in your target cells before...

25. "Build Your Plasmid": The Game - Play to Teach

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    Blog Post

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    July 17, 2018, 12:23 p.m.

    ... however you may use it and modify it for non-commercial reuse. There should be ways to adapt the game...

26. Learning Technical Skills at a Lab Central Workshop

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    Blog Post

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    Feb. 20, 2018, 2:30 p.m.

    ...my experience in my first blog post! I highly recommend doing the same if you ever go to a workshop because...

27. K. phaffii: Rising to the Occasion

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    Blog Post

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    April 4, 2023, 1 p.m.

    ...community. Phillips Petroleum Company first commercially developed K. phaffii in the 1970s with the goal...

28. Multiplexed Overexpression of Regulatory Factors (MORF) collection

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    Blog Post

    Published

    March 30, 2023, 1:15 p.m.

    ...its current format, the MORF library is most immediately useful for TF library screens. Previously engineered...

29. The MTA at Addgene

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    Blog Post

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    July 23, 2024, 2:17 p.m.

    ...allows plasmids to be used for both research and commercial purposes. It’s important to note that it’s the...

30. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

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    Blog Post

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    Sept. 19, 2017, 1:20 p.m.

    ...your experiences using photosensitizers in the comments below!   References 1. He, J., Wang, Y., Missinato...

31. Four Factors that Differentiate the Stem Cell Field

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    Blog Post

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    Sept. 28, 2016, 2:35 p.m.

    ...including Shinya Yamanaka, George Q. Daley, Christine Mummery, Lorenz Studer, and Deepak Srivastava--addressed...

32. 5 Great Apps for Lab Life

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    Blog Post

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    Sept. 22, 2016, 2:30 p.m.

    ...have a favorite lab app, please share it in the comments section below! Many thanks to our guest blogger...

33. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

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    Blog Post

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    March 24, 2016, 2:30 p.m.

    ...catalytically active and inactive CRISPR-Cas9 using asymmetric donor DNA." Nature biotechnology (2016). PubMed...

34. How to Lead a Great Meeting

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    Blog Post

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    Feb. 9, 2016, 3:30 p.m.

    ...send around a Google doc where people can make comments. At meetings, we just focus on the parts where...

35. Components of CRISPR/Cas9

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    Blog Post

    Published

    Feb. 2, 2016, 5 p.m.

    ... cut by Cas9, it must contain a PAM sequence immediately downstream (3’) of the site targeted by the guide...

36. Addgene Welcomes John Doench to the Advisory Board

    Type

    Blog Post

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    Aug. 13, 2015, 2:30 p.m.

    ...You can give Dr. Doench your own welcome in the comments section below! Special thanks to our sound engineer...

37. Management for Scientists: Delegating is Key

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    Blog Post

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    Feb. 20, 2015, 8:30 p.m.

    ... Agree on a way to review project progress. I recommend putting a regular check-in meeting on the calendar...

38. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

    Type

    Blog Post

    Published

    Aug. 28, 2014, 3:34 p.m.

    ...distribute. This method is time-intensive, so we recommend a variety of ways to screen and verify your plasmids...

39. Plasmids 101: Mammalian Vectors

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    Blog Post

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    March 25, 2014, 3:15 p.m.

    ...  Keep these tips in mind: There is not one recommended concentration for selection in mammalian cells...

40. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

    Type

    Blog Post

    Published

    Jan. 30, 2020, 2:15 p.m.

    ...before a study is conducted. Editors and reviewers recommend changes, and if everyone agrees that the research...

41. Hot Plasmids - February 2022

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    Blog Post

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    Feb. 22, 2022, 2:15 p.m.

    ...ladder system is a cost-effective alternative to commercial ladders which also provides a useful yet low-...

42. Sweating the Small Stuff: Details in the Lab

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    Blog Post

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    Feb. 8, 2022, 2:15 p.m.

    ...have another short "quiet period" instead of immediately running to the next thing.  It may seem counterintuitive...

43. Antibodies 101: Monoclonal Antibodies

    Type

    Blog Post

    Published

    June 29, 2021, 1:15 p.m.

    ..., Bestagno M, Burrone O, Maynard J, Ferrara F, Trimmer JS, Görnemann J, Glanville J, Wolf P, Frenzel A...

44. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

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    Blog Post

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    Feb. 19, 2019, 1:36 p.m.

    ...daughter. It’s super fun and we are enjoying that immensely. Paratore: I have two children active in sports...

45. Communicating Your Science With Help From ComSciCon

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    Blog Post

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    July 19, 2018, 1:12 p.m.

    ... it's not always recognized and valued for its immense importance, it may well be what determines whether...

46. Viral Vectors 101: Viral Vector Elements

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    Blog Post

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    July 25, 2023, 1 p.m.

    ...yield dsDNA. Unlike LTRs, ITRs are perfectly symmetrical to allow for this pairing to occur. Like LTRs...

47. GCE4All: Making Genetic Code Expansion Accessible

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    Blog Post

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    June 15, 2023, 1:15 p.m.

    ...could be useful in your research? We definitely recommend checking out GCE4All’s website and resources, ...

48. Viral Vectors 101: Viral Applications

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    Blog Post

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    April 13, 2023, 1:15 p.m.

    ...emerging viral applications and give you some recommendations for putting viruses to work. Viral applications...

49. Antibodies 101: Flow Cytometry Controls

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    Blog Post

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    June 6, 2024, 1:50 p.m.

    ... appropriate isotype controls linked on their commercial antibody pages. Flow cytometry can be a complicated...

50. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type

    Blog Post

    Published

    July 6, 2017, 1:59 p.m.

    ...in real time. Addgene depositor Professor Max Krummel from UCSF showed some impressive videos of these...