We narrowed to 488 results for: mme

...different role?  I think the secret sauce can be summed up in one word – initiative.  Initiative is the...

...knowledge-intensive workers”. I have added a few comments of my own to this list. Need feedback on their...

...backbone. Or just ask –  leave a message in the comments below and an Addgenie will help you find what ...

...ingenuity to solve this problem.  None of the commercial boxes were small enough to minimize the required...

... in ordering from ABRC for the first time, we recommend reaching out to us at [email protected] for help...

...mouse (NEB, E8032S) antibodies against MBP are commercially available. Factor Xa loves calcium, but metal-chelate...

... annotated boundaries for control elements in commercial plasmids were inconsistent and sometimes clearly...

...know which pooled libraries you’ve used in the comments! Resources Additional resources on the Addgene...

...something clarified? If so please, post in the comments.  References Chatterjee A, Sun SB, Furman JL, ...

...that you've been working on, let us know in the comments below! Additional resources on the Addgene blog...

...Limitations with GFP in imaging  While GFP has been an immensely powerful tool to study proteins in vivo, it does...

..., office of knowledge transfer, office of commercialization Additional resources on Addgene.org Addgene...

... and named the protein mTFP1. The researchers recommend using mTFP1 with a 445/30 nm excitation filter...

... (pAAV.hSyn-FLEX.iGABASnFR.F102G) is noticeably dimmer than iGABASnFR, and accumulates in the endoplasmic...

...large brain samples. For large brain samples, we recommend pretreatment with ScaleCUBIC1 (Susaki et al., ...

...model system is a mouse, given the wealth of commercially available mouse monoclonal antibodies. The good...

...from molecular and biochemical fundamentals to commercial systems. Appl Microbiol Biotechnol. (2003) 60...

...anti-Protein B antibody (Fig. 3)    Figure 3: Recommended secondary antibodies for rat IgG1 and mouse 1gG2a...

...relationships are all things almost all of us are programmed to appreciate and understand. We build narratives...

...using NgAgo? Let us know how it’s working in the comments section! Read About NgAgo User's First Impressions...

...piece (see NEB for more information). Thus, we recommend that you add 3-6 bases upstream of your restriction...

...In the summer of 2013, a remarkable nine papers describing CRISPR/Cas9 genome engineering methods for...

...interest that would be able to apply themselves immediately to the position. In case you are interested yourself...

.... Meet 10 PhDs with interesting careers, find a summer internship, etc.). It is helpful to work together...

...more about next. Leave your suggestions in the comments section.  ...

... however you may use it and modify it for non-commercial reuse. There should be ways to adapt the game...

...my experience in my first blog post! I highly recommend doing the same if you ever go to a workshop because...

...allows plasmids to be used for both research and commercial purposes. It’s important to note that it’s the...

... a DNA ladder with known concentrations. Many commercial DNA ladders will tell you the concentration of...

...send a wrong message for some people. We should recommend #PhysicalDistancing. Social interactions and #...

...including a terminal PDZ ligand. The researchers recommend using the SGZ for in-culture work only, GPI anchors...

...your experiences using photosensitizers in the comments below!   References 1. He, J., Wang, Y., Missinato...

...community know what your favorite book is in the comments! Additional resources on the Addgene blog Learn...

...! Control AAV For many AAV experiments, we recommend validating the serotype in your target cells before...

...community. Phillips Petroleum Company first commercially developed K. phaffii in the 1970s with the goal...

...its current format, the MORF library is most immediately useful for TF library screens. Previously engineered...

...including Shinya Yamanaka, George Q. Daley, Christine Mummery, Lorenz Studer, and Deepak Srivastava--addressed...

...have a favorite lab app, please share it in the comments section below! Many thanks to our guest blogger...

...catalytically active and inactive CRISPR-Cas9 using asymmetric donor DNA." Nature biotechnology (2016). PubMed...

...send around a Google doc where people can make comments. At meetings, we just focus on the parts where...

... cut by Cas9, it must contain a PAM sequence immediately downstream (3’) of the site targeted by the guide...

...You can give Dr. Doench your own welcome in the comments section below! Special thanks to our sound engineer...

... Agree on a way to review project progress. I recommend putting a regular check-in meeting on the calendar...

...distribute. This method is time-intensive, so we recommend a variety of ways to screen and verify your plasmids...

...  Keep these tips in mind: There is not one recommended concentration for selection in mammalian cells...

... it's not always recognized and valued for its immense importance, it may well be what determines whether...

...sometimes cofactors) to be provided and are generally dimmer than fluorescent proteins. On the other hand, they...

...ladder system is a cost-effective alternative to commercial ladders which also provides a useful yet low-...

...have another short "quiet period" instead of immediately running to the next thing.  It may seem counterintuitive...

..., Bestagno M, Burrone O, Maynard J, Ferrara F, Trimmer JS, Görnemann J, Glanville J, Wolf P, Frenzel A...