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...increasingly popular in fundamental and applied research since their first use in the early 90’s to genetically...inefficient at infecting non-dividing cells and so researchers set out to create a better, safer system. Unlike...than the first one and can be used routinely in a research laboratory. The risk of generating RCL is low ...technique, there’s always a small chance that a researcher could infect him or herself with the oncogenic... generation lentiviral vectors can be used for research and for clinical purposes, however improving the...safety of these vectors is still an active area of research due to the possibility that mutation or recombination...lentiviruses with human tropism can still infect the researcher and insert into her/his genome - this is a biosafety...

...10 blog posts from the year. 10. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage...plasmids every quarter. The “hot plasmids” from last March were quite popular and represent the diversity of...academic job interview including defining your research vision, organizing your vision into a chalk talk...many model organisms people are using for their research. In this blog post, we’ve covered five model organisms...yeast, zebrafish, and worm. 4. Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics... technique in molecular biology and scientific research. Get an overview of these methods in this blog...fantastic year and happy reading. Best wishes on your research in 2020! Additional resources on the Addgene ...

...Addgene.org Find plasmids for your research Find cancer research plasmids and resources ...represents a considerable barrier to furthering research and developing effective treatments for the disease...work, and how these plasmids can help cancer researchers studying an assortment of genes. Using patient...Anderson Cancer Center patient databases, this research team identified 1,049 perturbations in several...functional annotation of thousands of mutations The researchers used the collection to screen individual perturbations... platform is an incredible boost to the cancer research community, but what else are these plasmids useful...Some of the most heavily-studied genes in cancer research, like PIK3CA (Plasmid 116771: pHAGE-PIK3CA) and...

...  Easily search for plasmids (and more!) The main goal for the homepage was to improve search and browse...redesign, the new search bar is now front and center, with a clear call to action - Search! Along with this...It became clear to us that we needed to improve search and navigation, while also updating brand messaging...Community, sharing, and fun Along with improving search and navigation, we also wanted to emphasize the...the Addgene blog Learn more about Addgene's new search tool Read more about other features of Addgene'...website Resources on Addgene.org Try out the new search on Addgene's homepage ...

...in full-length publications. As early career researchers, we wondered how we could make the insights gained...around reading through entire papers, perhaps you search google images for specific experimental results...These data are valuable but remain hidden from research papers as they are not included in traditional...study with multiple patients was published, Zika researcher Christa Osuna collated Zika case studies, making... the NIH and Wellcome Trust now accept interim research products (pre-registration, preprints, data, software...micropublications) in grant proposals. ReFigures are one such research product which can convince reviewers of your expertise...recommend sharing ReFigures on your Linkedin and ResearchGate profile. To see how to create ReFigures in a...

...mission: Accelerate research and discovery by improving access to useful research materials and information...which we promote our mission and work to enable researchers around the world. We take this responsibility... by generating revenue through distribution of research materials. Our small shipping and handling fees...materials shared through Addgene are being used for research purposes and not for a profit motive. We build... we will always look for new ways to serve the research community. Questions? Feel free to reach out to...science and Addgene in our mission to accelerate research with easier sharing and collaboration. Joanne ...Nonprofit Seeding Labs Addgene Supports Rare Disease Research Listen to Our Interview with Addgene Board Member...

...your favorite search term, i.e. “biology + masters.” For lab-based roles, look for “research associate,”...have masters by using the search term “master’s degree.” Use the LinkedIn search filters to limit results...postings  Look at master’s-level job postings on a job search website like Indeed. Do the job descriptions sound...manuscript and grant preparation, and lead your own research project.  3. Do you prefer being a generalist?...variety of projects and in different fields of research is one of the best parts of my career with a master...master’s. While it's possible to change fields of research with a PhD, a master’s is less specialized so ...

...mechanism of incorporation is not well understood, researchers have been using AAV vectors for gene editing ...iPSCs from ~0.2% to ~1.5%. In more recent work, researchers have combined AAVs with CRISPR systems. Ohmori...an AAV’s ~4.5 kB packaging capacity. Luckily, researchers have devised methods to deliver CRISPR components...delivery method frees up space in the AAV allowing a researcher to fill it with the repair template. Bok and ...hematopoietic stem cells respectively. Future researchers could apply this technique to efficiently deliver... continue to use and develop AAVs for your own research and encourage to deposit any new AAV tools you...Cancer Center, Georgetown School of Medicine. His research currently focuses on determining the mechanisms...

...Plasmids from:  March (Q1) | June (Q2) | September 2016 (Q3) | December 2016 (Q3) MARCH 2016Enhancer activity... much literature searching, DNA synthesis, and plasmid assembly. Luckily, researchers from Douglas Densmore... states. Alexander Stark and colleagues at the Research Institute of Molecular Pathology have developed... library     CRISPR pooled libraries enable researchers to screen the entire genome for genes that regulate...newly available PRESTO-TANGO kit which allows researchers to interrogate the druggable human GPCR-ome using...utility of this platform for drug discovery. Researchers can use plasmids from the PRESTO-TANGO kit directly...-disciplinary Integration of Design Automation Research lab Moduclar Cloning (CIDAR MoClo) Parts Kit. ...

...database is to browse or search information in more powerful ways, and the advanced search page simplifies the...contributed by guest blogger Talley Lambert, a Research Associate at Harvard Medical School. The need ... most indispensable tools in modern biological research.  Any microscopist will tell you that selection...has been very useful over the years, and when researchers come to us for advice on choosing a protein, ...particular FP; this can be done directly using the search bar on the homepage (and at the top of all pages...the process of building advanced multi-parametric search queries.  Looking for a bright, monomeric FP published...guest blogger Talley Lambert! Talley Lambert is a Research Associate at Harvard Medical School. He manages...

... results helps other researchers using these plasmids and will give your research more visibility.    ...By Will Arnold and Shreya Vedantam Accelerating research by improving the availability of materials is ...to help academic and industrial labs accelerate research and to help find a faster solution to the COVID...coli and S. cerevisiae expression vectors in late March. The first set of 383 constructs went online just...samples, the rest will be put into a status we call “Archived.” This status allows us to store plasmids and ... is indeed global. Helping scientists speed up research is what we’re here for and we’re glad to be able...Ginkgo Bioworks  Find COVID-19 plasmids for your research Browse the Ginkgo Bioworks COVID-19 Plasmid Collection...

...A 2017 survey found that many researchers feel they lack formal training in a variety of transferable...out to fill this gap by both highlighting that researchers do learn MANY transferable skills while working... into play while puzzling through your broader research questions and designing future experiments. Being...While I was in graduate school and beginning to research non-academic careers, I always felt that descriptions...when you’re faced with yet another hurdle in your research journey.  What are ‘problem solving skills’? As... about the world. You might do some background research to better understand it and come up with several... of your skill set later on. If you are new to research and the lab, you may not be able to carry out ...

...represents a unique funding model in nuclear receptor research, and has generated a set of comprehensive datasets... questions in nuclear receptor and coregulator research. Our Q-PCR datasets, for example, document the... today.  The sheer abundance of these critical research tools is a double-edged sword, however, and has...burden associated with identifying plasmids in the research literature and acquiring them in a timely manner...to the problem of sharing plasmids, providing researchers with a one-stop shop for plasmids and vectors...vectors, and making them available to all researchers for a reasonable fee. We at NURSA recognize the importance...importance of this service to the NR research community and, accordingly, the new version of the NURSA website...

...off-target cleavage events. Unbiased techniques search the whole genome for off-target sites irrespective... in concert, these approaches can provide the researcher with a reasonable level of certainly that the...sgRNA. This is a good starting point for most researchers as it provides a list of putative off-target ...can later sequence for mutations. One method a researcher can use to test predicted off-target sites following...to perform these validations as translational researchers begin to use CRISPR as a therapy. Low frequency... effects may generate irreproducible data in a research setting, but these events could have disastrous...off-target prediction algorithms, their genome-wide search criteria are not exhaustive. Mismatch tolerance...

... most commonly used research tools in scientists’ pockets. They allow researchers to identify and isolate...an antibody that will be essential to your new research project, but unsure which one will mark your target...bind to the target). Unreliable antibodies cause research that cannot be reproduced and projects that cannot... with collaborators and support from the Brain Research Through Advancing Innovative Neurotechnologies...Dr. Wu please contact us here! Ava Shipman is a research assistant in lab of Zhuhao Wu at the Icahn School...Reproducibility: Standardize antibodies used in research. Nature 518, 27–29 (2015). https://doi.org/10.1038...The Economics of Reproducibility in Preclinical Research. PLoS Biol 13(6): e1002165. https://doi.org/10.1371...

...on a stochastic search algorithm rather than pre-designed sets, so identical searches can return multiple...particularly for high-complexity reactions? The researchers over at New England Biolabs® thought otherwise...design (DAD) to build out three tools to help researchers design high-fidelity Golden Gate Assemblies and...three tools developed, outlined below, allow for researchers to select large sets (with overhangs that violate...You can define the number of fragments, and the “search windows” you’d like the tool to use to identify... can exclude specific sequences or set a narrow search window for a site—or sites—that must be used. Each...necessarily require cycling. The Pros and Cons Researchers used the tools above to complete a 52-piece assembly...

...students work closely with local researchers to learn about their research while creating physical models...graphics packages were soon developed that allowed researchers to create stunning, interactive images of their... only wonder what I am talking about. How do researchers use physical models of protein structure? We ...been creating physical models of proteins for researchers for the past fifteen years. On those occasions...following sequence of events plays itself out: The researcher breaks eye-contact with any other human in the... and to create custom models of proteins for researchers. Modeling CRISPR We were excited when Jacob Corn...strand. And this is why we love working with researchers on a model. The little knowledge that we have...

...plasmid from our search page (check out this video for a walkthrough of the ways you can search our website...trail at the top of any plasmid page gives the hierarchy of web pages the plasmid page is nested in. You...website). A quicker way to find this plasmid through search is... The Addgene plasmid number: This is the unique...know the catalog number of the plasmid you are searching for, this is the quickest and easiest way to find...than 50; and blue, more than 100 times! When you search for plasmids on our website, one of the available...see if the plasmid would be useful for their own research, and helps requestors use the plasmid in their...citations for Addgene plasmids you have used in your research! Sequence information: Depositors may provide ...

...typically a professor who operates a research group. Within that research group, you will also typically receive... waiting for you, prep for research work has to be done by the researchers themselves.  Be mindful ...more, depending on the mentoring style within the research group). The bench mentor-mentee relationship ...relationship will probably define your experience in the research group more (at least at first) than any other ...Getting the most out of the training experience In research there is a saying that if the sample prep is poor...fulfilling experience; even the grumpiest of researchers can enjoy it at times! What makes it rewarding...

...Initiative being to cure cancer, more funding and research opportunities are being provided to immunotherapy...Therefore, mesothelioma doesn’t receive nearly as much research funding from the government as more common cancers...breast cancer (about 246,000 diagnosed this year). Research efforts in mesothelioma instead rely upon private... they are exposed even one time. Doctors and researchers don’t entirely understand why some people develop..., along with other forms of cancer. Additional research is being done to see what, if any, other genetic...immunotherapy, and angiogenesis inhibitors New research is also going into two other areas that could ...than ever. From breath tests to blood tests, researchers are hopeful that they can come up with new ways...