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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 322 results for: BRE

Showing: 121 - 140 of 322 results

  1. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ...on studies in many disciplines has been a long brewing frustration for me. I read a variety of psychology... missing out on. Yet I believe that the big breakthroughs in my discipline, education, will come from ...

  2. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    Published
    Oct. 20, 2016, 2:30 p.m.
    ...variety of topics. Individual forums are hosted on subreddits (r/”topic”) which are essentially lists of forums...engaged, and has great questions! 2. The r/Science subreddit is well moderated. One of our original worries...

  3. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    Published
    Oct. 13, 2016, 2:30 p.m.
    ... healthcare consultant and what you could do to break into the field. What is healthcare consulting? ...from taking up such complex challenges, quickly breaking them up into smaller achievable tasks, and then...

  4. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...gRNA Figure 1: Validation of double strand break (DSB) mediated homology dependent repair by enhanced...better that gRNA is at causing a double stranded break at the target site, allowing the two halves of EGFP...

  5. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    Published
    Nov. 5, 2014, 3:41 p.m.
    ...offense to BRET, CRET and other RET techniques which have never outclassed the Master. Note: BRET= Bioluminescence...

  7. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    Published
    March 27, 2018, 1:32 p.m.
    ...frameshift mutations through aberrant double strand break repair, it has proven more challenging to develop...editing via the introduction of a double strand break by using a targeted nuclease. Asuri et al, for example...

  8. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    Published
    Aug. 2, 2016, 2:30 p.m.
    ... (HDR) of a CRISPR/Cas9 induced double-stranded break. This site-directed genome editing requires a template...researcher at Cornell University.       References 1. Breslauer, Kenneth J., et al. "Predicting DNA duplex stability...

  9. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    Published
    June 24, 2020, 5:45 p.m.
    ...to specific loci without inducing double strand breaks. Below you'll find some of the CRISPR-based epigenetic...programmable and targeted de novo DNA methylation in breast cancer. Oncogene 34:5427–5435 . https://doi.org...

  10. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    Published
    July 12, 2016, 2:30 p.m.
    ...Part 1: Genetically Engineered Mice and Part 2: Breeding and Crossing Mice. My first CRISPR mouse experiment...endonuclease that creates the DNA double-strand break (DSB) at the target site, and a donor oligonucleotide...

  11. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    Published
    May 31, 2016, 3:30 p.m.
    ...standard DNA plasmid transfection, a double-stranded break may occur in your cargo. Additionally, with piggyBac...as CRISPR/Cas9) to create a double-stranded DNA break. After DNA cleavage, the piggyBac™ donor is used...

  13. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ...allowing for easy multiplexing. The double-stranded breaks caused by Cas12 create short 3’ overhangs, which...hypothesis, and even the time and money available. The breadth and diversity of CRISPR systems will no doubt keep...

  15. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    Published
    Sept. 3, 2020, 12:15 p.m.
    ...Nihongaki, et al., 2015) resulting in a double strand break (DSB) that can be repaired by either non-homologous...References Bubeck F, Hoffmann MD, Harteveld Z, Aschenbrenner S, Bietz A, Waldhauer MC, Börner K, Fakhiri ...

  16. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...targeted known residues on the outer surface to break dimerization interfaces (affecting many of the RFPs...of cytosine to thymine without a double stranded break. However, the method was initially limited by off-target...

  17. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...Single Strand Annealing (SSA) in the event of a DNA break. Next, the gRNA being tested is expressed along ... simplifying experiments when additional cross-breeding steps are undesired or infeasible. Finally, an...

  18. A Case Study of Addgene Bioinformatics

    Type
    Blog Post
    Published
    May 25, 2023, 1:15 p.m.
    ... improve and redesign our pipeline. In it, they break down the unique challenges of our platform and how...
Showing: 121 - 140 of 322 results