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We narrowed to 593 results for: ELP

Showing: 121 - 140 of 593 results

  1. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type
    Blog Post
    Published
    Feb. 18, 2014, 4:22 p.m.
    ...who met every other Thursday for 25 years. They helped one another navigate career changes and overcome...about peer mentoring. They want to find mentors to help them develop their skills and to hold them accountable...brings a diversity of perspective. Sometimes it is helpful to find peers that share a similar direction or...advice. Attend a local networking event together and help each other practice meeting people – compete for...

  2. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    Published
    Sept. 24, 2019, 12:55 p.m.
    ...cloning experiment. Here are some tips that will help you with your cloning project, and hopefully obtain...possible). There are many softwares out there than can help you at this stage and that can be used to simulate...simulate in silico cloning. This will definitely help in understanding if your strategy will be successful...or human. There are several online tools that can help you with that, they can be open source (i.e. primer3...

  3. How to Prepare for an Industry Interview

    Type
    Blog Post
    Published
    July 9, 2020, 1:15 p.m.
    ...little overwhelming, but here is what I’ve found helpful for my interviews in industry. Prepare stories ...questions. Writing a rough outline of these stories helped me remember them for the interview.  Prepare a ... by looking at their LinkedIn profiles. This can help spark a more natural conversation while also demonstrating...can use notes you took during your interviews to help craft these thank you's. Interviewing remotely Remote...

  4. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type
    Blog Post
    Published
    June 25, 2020, 1:15 p.m.
    ...intern. As a member of the Quality Control team, I help make sure the plasmids that go out our doors are...considering my future path. Talking to Addgenies has helped me get a much better idea of what lies ahead. Thus...up for failure. Listening to Kenkel’s story has helped me realize that is not true at all. Racheal Komuhendo...gave her the balance she needed. It is incredibly helpful to me to know that you can make big changes in ...

  5. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ...heard that getting into industry early would only help. Like any major life decision, the transition felt...that environment sooner rather than later can be helpful. Publishing: As a postdoc at AstraZeneca, you are...varies between companies. Like most jobs, it does help if you know someone who’s already in industry to...Both paths will give you myriad opportunities to help you with the next step in your career.   Many thanks...

  6. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    Published
    Feb. 4, 2016, 3:30 p.m.
    ...kinds of information on our plasmid pages? Well, to help make the most of these pages, we've written this...plasmid would be useful for their own research, and helps requestors use the plasmid in their own labs. We...additional resources at the bottom of the page that help you cite this publication in your own work: How ...plasmid pages, please don't hesitate to contact us at help@addgene.org Resources on the Addgene Blog Read...

  7. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    Published
    Aug. 30, 2022, 1:15 p.m.
    ...those steps and provide you with more details to help you plan and optimize your own protocols. Here we...Antibodies are large proteins that need a little help crossing cell membranes as they are not able to ...permeabilize non-selectively. This non-selectivity is helpful when trying to pass tough-to-permeabilize membranes...This article on fixation has some more examples to help choose which fixative to use!) Ultimately, the ...

  8. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type
    Blog Post
    Published
    Dec. 3, 2019, 11:38 a.m.
    ...our scientific technical support at help@addgene.org and let us help.  Check back on Thursday for an inside... we’ve built a new and improved search engine to help make this task a faster, easier, and frustration-free...define for a simple search. These pages provide helpful information about the subject, link to related ...

  9. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ... health and medical organizations are working to help educate the public about proper antibiotic usage...Importantly, native, non-pathogenic bacteria remain to help recolonize the niche, reducing the chance of an ...replication-incompetent phage particles using a helper system. These CRISPR antimicrobials successfully...Future research will show if this new strategy can help us win back ground in the fight against bacterial...

  10. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ...function. Small ubiquitin-related modifier (SUMO) can help with folding and stabilization, as can maltose-binding...aggregated protein is not useful protein. MBP tags can help with solubility issues, but scientists may also ...that improve disulfide bond formation in order to help keep your protein soluble.  Tags for affinity and...described in the post, but have been categorized to help you better assess their function. More detailed ...

  11. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    Published
    Nov. 3, 2022, 12:15 p.m.
    ...trying to introduce a homozygous mutation, it’s helpful to know how many copies you are looking for. Secondly...untreated population. Software such as CRISPResso can help with the data analysis. If NGS is not an option,...prediction analyses like CRISPRitz or CRISPOR can help narrow down the list of sites where off target editing... at Addgene: Read our CRISPRGuide Get practical help from our CRISPR Protocols Find gRNA Design Tools...

  12. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ... sequencing can be done, this technique can also help pinpoint the genes within a sequenced genome that...for 1 h at room temperature. After this, it is helpful to subject your membrane to several freeze/thaw...remaining live E. coli. At this point, it can really help to incubate the membranes overnight in 500 mM to...specifically bind your target organism, it can be helpful to spot this protein directly onto nitrocellulose...

  13. Introducing the Addgene Antibodies 101 eBook!

    Type
    Blog Post
    Published
    Oct. 29, 2024, 1:15 p.m.
    ..., the Antibodies 101 eBook is designed to be a helpful desktop reference. Each chapter brings together...cytometry experiment, “Chapter 7: Sorting” is there to help guide you through the process. If you’re confident.... And, as always, don’t hesitate to reach out to help@addgene.org with any thoughts, questions, or feedback...

  15. The Codon Guide to Synthetic Biology

    Type
    Blog Post
    Published
    Sept. 14, 2023, 1:15 p.m.
    ...Guide to Synthetic Biology in the hopes that it will help orient others as they begin their journey into synthetic...YouTube channels, books, podcasts, and communities to help people get started in synthetic biology. While not...if you have additional resources that might be helpful, please reach out to me by email.  About the author...

  16. Alex Chavez on MD PhDs, CRISPR, Gene Drives, & More

    Type
    Blog Post
    Published
    Nov. 15, 2016, 3:30 p.m.
    ...member of the Addgene advisory board where he will help shape Addgene's future. Listen to this episode for... Wyss Institute. 33:09 - end : How Alex hopes to help Addgene advance - plants for trimmed down protocols...from Grad Student to Entrepeneur Find Resources to Help You Choose Your Career Path Resources on Addgene.org...

  17. Addgene’s Blog Gets a New Look!

    Type
    Blog Post
    Published
    Jan. 25, 2021, 5:54 p.m.
    ...new Addgene blog! Same URL, same great articles to help you with your experiments, but a new look. We started... Each topic category contains a overview page to help you get aquatinted. Here, you’ll see a summary of...topic you’d like to see, protocol tips that would help scientists, or a scientist we should interview. ...

  18. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ...combination of interest (Figure 1). It can therefore be helpful to try all three when looking to label an intracellular...can be time-consuming and expensive, it may be helpful to find an antibody that has already been validated...labeling them. We find that cell tracker dyes are helpful in designing such panels, so that pre-stained cell...information on tested antibodies against the target. Helpfully, this also includes clone names, which can allow...

  19. The Addgene Antibody Data Hub

    Type
    Blog Post
    Published
    Sept. 12, 2024, 1:15 p.m.
    ...Antibody Data Hub Addgene’s Antibody Data Hub can help with this decision. In the open-access Data Hub,...requesting an Addgene antibody, the Data Hub can help you decide if an antibody is a good candidate for...love all data, including negative data, which can help researchers save time and money when selecting an...

  20. Michael Koeris' Journey from Grad Student to Entrepreneur: The Story of Sample 6

    Type
    Blog Post
    Published
    Jan. 21, 2016, 4:52 p.m.
    ...venture capital firm, Flagship Ventures, Koeris helped change Novophage's focus. The company went from...on the Addgene Advisory Board and how he hopes to help young biotech entrepreneurs.   We'd like to thank...to watch both Addgene and Sample 6 grow with his help! Special thanks to our sound engineer/producer, ...
Showing: 121 - 140 of 593 results