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We narrowed to 253 results for: Gnas

Showing: 121 - 140 of 253 results
  1. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    Published
    Aug. 10, 2023, 1 p.m.
    ... an enzyme co-factor, a crosslinking agent, a signaling ion, and an allosteric modulator. Because of its...pdb-top069609. Palmer AE, Tsien RY. Measuring calcium signaling using genetically targetable fluorescent indicators...
  2. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...  Figure 2: Specific binding of scFVs to cognate aaRS antigens as measured by suspension bead assay...enriched in the pre-synaptic terminal providing better signal to noise for imaging calcium dynamics in fine structures...
  3. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    Published
    May 15, 2014, 3:33 p.m.
    ...light to detect, measure, and control molecular signals, cells, and groups of cells in order to understand... into distinct populations based on fluorescent signal. Thus, FACS can be used to separate cells expressing...
  4. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ...non-exhaustive list includes: Codon abundance relative to cognate tRNA abundance Repetitive sequences Restriction...conservation of codon optimality reveals hidden signatures of cotranslational folding." Nature structural...
  5. Career Insights: Technical Support Specialist

    Type
    Blog Post
    Published
    Nov. 9, 2017, 2 p.m.
    ...Blot protocol and then blame the antibody when no signals are obtained. Or someone may heat an ELISA plate...during incubation steps in order to enhance the OD signals and then complain about high background. In many...
  6. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    Published
    Jan. 24, 2023, 2:15 p.m.
    ...-expressing E. coli. The GV-specific ultrasound signal inside the tumors was overlaid with the anatomical... in situ expression. The GV-specific ultrasound signal is in hot and the anatomical B-mode images are ...
  7. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    Published
    Oct. 20, 2016, 2:30 p.m.
    ...They also host AMA forums where users can ask designated scientists anything about a particular topic....was writing all day and straight on through her designated two hours for answering questions. She was able...
  8. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    Published
    June 3, 2021, 1:15 p.m.
    ...SunTag scaffold, the intensity of the fluorescent signal is boosted and enables single molecule tracking... T cell receptor portion of the CAR facilitates signal transduction and release of cytotoxic granules ...
  9. Five Popular Model Organisms

    Type
    Blog Post
    Published
    April 11, 2019, 12:39 p.m.
    ...including the nervous system, development, cell signaling, aging, and mechanotransduction to name a few....Leifer’s lab utilized the worm to study calcium signaling in the entire adult brain, using a plasmid that...
  10. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ...binding sites found on avidin are also ideal for signal amplification during these experiments. During ...interest leading to amplification of the reporter signal and easier detection. This strategy is referred...
  11. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ...microinject into blastocysts to be implanted in pseudopregnant female mice. If you obtained the desired chimeric...microinjection, i-GONAD is performed directly on pregnant female mice using a small incision that exposes...
  12. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    Published
    March 21, 2017, 2:30 p.m.
    ...particle (the packaging signal Psi). Because the packaging plasmids lacked the psi signal, the genes encoding...
  13. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ...explanations for RNAi not reducing the antibody signal include: The antibody is not selective The knockdown...pass type-1 membrane protein), which has been designated CD365 in the most recent HLDA workshop (HLDA10...
  14. Your Top Requested Plasmid in 2015!

    Type
    Blog Post
    Published
    Dec. 30, 2015, 3:30 p.m.
    ...already been used for includes work probing mTOR signaling in cancer cells, deciphering the mechanism of ...
  15. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    Published
    Jan. 11, 2017, 8:47 p.m.
    ...Protocols Paper for more information). V2.0 This designation separates pSpCas9(BB)-2A-Puro (PX459) V2.0 from...
Showing: 121 - 140 of 253 results