We narrowed to 388 results for: HAL

... tool by splitting cGAL4 in two and binding each half to a gp41-1-N-intein. Split inteins are protein ...With the split system, only cells that have both halves of cGAL can drive expression of your gene of interest...

...Tebo et al. Nat Commun. 2019 Arnaud Gautier HaloTag TagBiFC allows exogenous labeling for single-molecule...and reversible complementation. TagBiFC (split HaloTag) provides maximum flexibility in color labeling...

...particularly hard. There are millions of them. The real challenge is finding relevant papers. The latest installment...topic or that were published by the lab down the hall.  Scientific journals: They publish papers, so naturally...

...tasks, both of which are factors in the technical challenges they can present. Many researchers find themselves...after transfecting cells, replace the medium with half the volume typically used (e.g., use 5 mL medium...

...processing and contributes to variability in RNA half-life, and ultimately gene-expression. Polyadenylation...39322032   No terminator is 100% efficient at halting transcription of the template and initiating the...

...real-time, posing a huge challenge to studying epigenetic changes over time.  This challenge was difficult to ...

... of your supervisor’s management style, bring challenges to his or her attention as soon as you can. It...mutually beneficial solutions. If you package your challenges with your supervisor into learning opportunities...

... publish or attend conferences makes it very challenging to compete in today’s tough academic or industry...team. We have confidence that we can take on any challenge and quickly become experts in something, even ...

...Turning on the UV light 3-4 times a week for up to half an hour can help keep your hood contamination free...Additional Resources on the Addgene Blog The Challenges of Cell Culture Cloning Mammalian Cells with ...

...incubator, additional growth medium is added to about halfway up each well. Tips for using the agarose cloning...Time CRISPR User (by a 1st Time CRISPR User) The Challenges of Cell Culture Plasmids 101: Mammalian Vectors...

...understand how protein localization changes over time.Hallmarks of fluorescent protein timers When FP timers...noticed a few properties that have now become hallmarks of ideal FP timers: 1. Ratiometric determination...

...of interest is essential, a true knockout can be lethal, and you'd instead want to create a conditional...depositing with Addgene! Figures were adapted from: Hall B, A Limaye, & AB Kulkarni. (2009) "Overview: Generation...

...this complex, developing Cas3 tools has been challenging. It can be difficult to ensure comparable expression..., though consistent expression can still be a challenge. A multitasking Cas Most Cas effectors have one...

...program itself. Picking a mentor comes with many challenges, and the flood of interview requests and visitation...a travel day to an online call in response to challenges on their end. Though the quick changes in plans...

...daily, yet obstacles remain. One of the biggest challenges facing researchers today is the complexity of... of the latest resources? In response to this challenge, Addgene has launched an initiative to curate ...

...entirety of a cell. That's a big long-term technical challenge. We have a strategy that organizes how we start...rebuilding existing genomes.) BIOFAB is laying down a challenge to its users and to the design community: If you...

...samples in a given state or point in time. You want to halt degradative processes while maintaining the structure...fixation-artifacts-and-how-to-minimize-them/  Goldenthal KL, Hedman K, Chen JW, et al (1985) Postfixation...

... Biology (Kimball et al., 2020). Benefits and Challenges CUREs inspire students to take ownership of their...experience on their resumes). CUREs are not without challenges. Real research is messy, and it can be difficult...

...generating the standard curve for qPCR can be challenging to get right. Here at Addgene, we use a linearized...droplet digital PCR for vaccine evaluation in challenged monkeys and TB diagnosis." Emerging microbes ...

...best plasmid for your research can be a daunting challenge. With over 80,000 deposited plasmids from around...applying a filter for “CRISPR” plasmids keeps more than half of these. Instead we can navigate first to the CRISPR...