We narrowed to 253 results for: IRES

1. Celebrate Open Data Day with Addgene’s AAV Data Hub!

   Type

   Blog Post

   Published

   March 5, 2020, 2:15 p.m.

   ...on our blog. Image: The final and required step requires submission of an experimental image result that...

2. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

   Type

   Blog Post

   Published

   June 17, 2021, 1:17 p.m.

   ...The original AraC system, which uses arabinose, requires many wash steps to remove arabinose making it ...

3. Using Addgene's New Viral Service for Your Research

   Type

   Blog Post

   Published

   Nov. 17, 2016, 3:30 p.m.

   ...Virus production can be a frustrating process that requires a large investment of time and resources for labs...

4. Plasmids 101: Origin of Replication

   Type

   Blog Post

   Published

   Nov. 11, 2020, 2:23 p.m.

   ...varies. See below for additional considerations. *Requires pir gene for replication (reference). **F1 is...

5. Summer SciComm: Science Storytelling

   Type

   Blog Post

   Published

   Sept. 16, 2022, 1:15 p.m.

   ...opposing forces or (2) the result of competing desires or (3) the presence of obstacles that need to be...

6. RUBY-Red Siliques

   Type

   Blog Post

   Published

   March 1, 2022, 2:15 p.m.

   ...plants, but screening these can be challenging and requires specialized equipment. Reporters for studying ...

7. Plasmids 101: TOPO Cloning

   Type

   Blog Post

   Published

   Oct. 27, 2016, 2:30 p.m.

   ...does not use restriction enzymes or ligase, and requires no post-PCR procedures. Sounds easy right? The...

8. Trading Spaces - Visiting Scientist Edition

   Type

   Blog Post

   Published

   Aug. 22, 2023, 1 p.m.

   ...from equipment sharing or a collaboration – it requires re-location of the scientist to the new institute...

9. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

   Type

   Blog Post

   Published

   June 30, 2020, 1:15 p.m.

   ...pointed out that networking at virtual conferences requires you to take more initiative. “You have to actively...

10. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

    Type

    Blog Post

    Published

    Dec. 10, 2019, 1:58 p.m.

    ...transcription. Transcription from σ54 promoters requires binding of bacterial enhancer binding proteins...

11. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type

    Blog Post

    Published

    Oct. 26, 2017, 1:59 p.m.

    ...a powerful research tool, but their proper use requires careful amplification and maintenance of good ...

12. The Power Behind NGS Plasmid Validation: seqWell

    Type

    Blog Post

    Published

    April 19, 2017, 3:25 p.m.

    ...limited by costly library prep, as each sample requires an individual library. The plexWell™ workflow ...

13. Science Career Options

    Type

    Blog Post

    Published

    Feb. 23, 2017, 1:30 p.m.

    ... Experience in the field or in a position that requires travel can show that you are self organised, reliable...

14. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type

    Blog Post

    Published

    Sept. 19, 2017, 1:20 p.m.

    ...longer exposure times. Additionally, KillerRed requires mitochondrial targeting to increase cell ablation...

15. Visualizing Genomic Loci with CRISPR-Sirius

    Type

    Blog Post

    Published

    April 18, 2019, 12:53 p.m.

    ...different chromosomes in living cells, the system requires high-copy chromosome-specific loci in the genome...

16. Improving Transduction Efficiency with Exosome AAVs

    Type

    Blog Post

    Published

    March 14, 2019, 1:54 p.m.

    ...delivery of therapeutic proteins to the brain which requires crossing the blood-brain-barrier. In summary, ...

17. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

    Type

    Blog Post

    Published

    Sept. 3, 2019, 1:15 p.m.

    ...nuclease. The Streptococcus pyogenes Cas9 (SpCas9) requires an NGG PAM to recognize the target. Osamu Nureki...

18. 5 Great Apps for Lab Life

    Type

    Blog Post

    Published

    Sept. 22, 2016, 2:30 p.m.

    .... The tools are a lifesaver when your research requires cell culture and include a counter, lab timer,...

19. From Lab Bench to Leadership: Transitioning into Management as a PhD

    Type

    Blog Post

    Published

    Jan. 21, 2025, 2:15 p.m.

    ... goals, and fostering team success. This shift requires deliberate preparation and a willingness to embrace...

20. Viral Vectors 101: The AAV Retrograde Serotype

    Type

    Blog Post

    Published

    April 27, 2023, 1:15 p.m.

    ...any genetic tool delivered by the AAV.   AAVrg requires high titers for efficient retrograde transport...