We narrowed to 461 results for: MEA;
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TypeBlog PostPublishedJan. 21, 2016, 4:52 p.m....products as opposed to therapeutics 15:15 - 16:26 : Means for testing for pathogens in food products prior...being an entrepreneur 29:00 - 32:20 : What did It mean for Dr. Koeris to personally take on the risk of...
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Plasmid Preps: Different Purity, Different Quantities, Different Uses
TypeBlog PostPublishedJune 16, 2020, 1:25 p.m.... the absorbance measurements at 230, 260, and 280 nm. The absorbance ratios measured at 260 and 280 nm... -
Fluorescent Proteins 101: History of Fluorescent Proteins
TypeBlog PostPublishedAug. 7, 2017, 1:58 p.m.... useful tools because we can easily detect and measure the light they emit. Proteins that give off light...this new green glowing protein could be used to measure gene transcription, as well as to track protein... -
Science Communication for Everyone
TypeBlog PostPublishedApril 16, 2019, 1:05 p.m....manipulate” could mean “illicit tampering” to general audiences while “manipulate” could mean “worked with”... -
Top Requested AAV of 2017: pmSyn1-EBFP-CRE
TypeBlog PostPublishedJan. 17, 2018, 2:57 p.m....in neurons. Indeed, this AAV provides you with a means to specifically activate expression of any Cre-dependent...Titers: Where do they come from and what do they mean? AAV: A Versatile Tool for Gene Expression in Mammals... -
Management for Scientists: Seeking Feedback
TypeBlog PostPublishedJan. 8, 2015, 2:34 p.m....on the team talks and listens in roughly equal measure, keeping contribution short and sweet. One key...team. We are not talking about gossip here – this means encouraging a culture of maximum information flow... -
From our Table to Yours: An Inside Look at Lunch at Addgene
TypeBlog PostPublishedNov. 27, 2013, 2:54 p.m....Addgene nearly 10 years ago, lunch felt like a family meal. My co-founders actually were my family (Kenneth... forward to gathering around a large table for a meal with my family. I am thankful for many things, and... -
CUT&RUN: An Improved Method for Studying Protein-DNA Interactions
TypeBlog PostPublishedFeb. 13, 2018, 2:51 p.m....significant being the usage of a strong detergent to permeabilize cells rather than to rely on nuclei extraction...desired. It also provides an excellent additional measure of DNA binding sites in combination with existing... -
Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes
TypeBlog PostPublishedJune 19, 2018, 1:38 p.m.... protein shell that excludes water but is gas permeable. These vesicles are primarily used by water-dwelling... of arg1 and arg2 ultrasound signals. This also means that multiplexing is possible if bacteria are sequentially... -
The Blue Flame Award: Celebrating Addgene's Most Requested Depositors
TypeBlog PostPublishedNov. 14, 2016, 3:30 p.m....times A red flame means a plasmid has been requested 50+ times A blue flame means a plasmid has been... -
Five Questions to Ask If You're Deciding Between a Master's or PhD Program
TypeBlog PostPublishedFeb. 16, 2021, 2:15 p.m....in master’s-level jobs? Ultimately a degree is a means to gain employment, so it’s important to make sure... for PhDs, both in academia and industry, often means moving from the lab to a desk, although there’s ... -
Plasmids 101: Antibiotic Resistance Genes
TypeBlog PostPublishedJan. 30, 2014, 3:29 p.m.... 1 out of 10,000 cells on average! Without some means of quickly determining which cells successfully ...Polymyxin B polypeptide Alters outer membrane permeability Bactericidal 10-100 ug/mL Tetracycline tetracyclin... -
Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring
TypeBlog PostPublishedNov. 12, 2019, 2 p.m....temperature readings using a heat gun, and pH measurements. We collected small water and sediment samples...samples and move farther away until the bison meandered off. In the end we were able to safely get back... -
Intro to Our Summer SciComm Series!
TypeBlog PostPublishedJune 9, 2022, 1:15 p.m....other uses. Many laypeople would use “PCR test” to mean “a test that uses a biological sample to look for...for presence of a virus.” A scientist would likely mean “a test for the presence or absence of a predefined... -
New and Upcoming Viral Vectors - December 2019
TypeBlog PostPublishedDec. 19, 2019, 2:40 p.m....spatiotemporal resolution required for precise in vivo measurements of dopamine (113049, 113050) or norepinephrine...Looger’s lab at Janelia for neuroscientists to measure glutamate levels. iGluSnFR.A184S, a variant with... -
Hot Plasmids: FRET-Based Biosensors
TypeBlog PostPublishedMay 6, 2014, 1:07 p.m....toxic,” Merkx explained. New tools were needed to measure them. Merkx says, while in principle adapting a...them,” Merkx said. “For us, it is one of the main measures of success – whether we have really developed ... -
Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS
TypeBlog PostPublishedJuly 21, 2017, 12:48 p.m....process of generating your own fly lines. In the meantime, be sure to browse the publication references ...Brand, N. Perrimon, Targeted gene expression as a means of altering cell fates and generating dominant phenotypes... -
The Power Behind NGS Plasmid Validation: seqWell
TypeBlog PostPublishedApril 19, 2017, 3:25 p.m....multiplexed pool. The cost of these normalization steps, measured in time and consumables, can be daunting for large...normalized to two-fold variation above and below mean read count per sample, and the amount of information... -
Visualizing Genomic Loci with CRISPR-Sirius
TypeBlog PostPublishedApril 18, 2019, 12:53 p.m....interest, the team could visualize three loci and measure inter-locus distances. They tested this system ...intergenic DNA region (IDR3) as a reference loci to measure inter-locus distances of loci pairs from intergenic... -
Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish
TypeBlog PostPublishedNov. 27, 2018, 2 p.m....deletions on biofilm formation and growth will then be measured to link the deleted genes to specific functions...river, take water samples at specific locations, measure fluorescent signals from the sensor, and send a...