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We narrowed to 479 results for: RET

Showing: 121 - 140 of 479 results

  1. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ... (a British idiom for “taking by surprise”) by Margaret Robinson’s lab, and more recently used by Stephen...Opto-RTK constructs – Opto-mFGFR1, Opto-hEGFR, and Opto-hRET – as well as various LOV-domain-mVenus constructs...directionally assemble in a single reaction, while retaining only a defined 4bp fusion site in between. The...vectors with the desired sequence of interest are retained after SLIC cloning. The general cloning strategy...

  2. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    Published
    March 8, 2021, 2:41 p.m.
    ...copper cathode/iblot gel matrix, and filter paper. Pretty much like an actual sandwich! S'mores image from....2015.04.012 Burnette WN (1981) “Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide....76.7.3116 Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels...

  3. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    Published
    May 31, 2016, 3:30 p.m.
    ...high-throughput splinkerette-PCR method for the isolation and sequencing of retroviral insertion sites...during the differentiation process. Moreover, a splinkerette-PCR assay may be used to map the specific genetic...

  4. Finding Your Perfect Job After University

    Type
    Blog Post
    Published
    Jan. 12, 2016, 3:30 p.m.
    ...experience. Working in industry: Microbiology On returning from Ghana at the beginning of 2010, I explored...looking for other career options after 6 months. Returning to university to study a Masters of Science I ...programming. Working for a scientific nonprofit On returning to the UK, I checked out the job postings from...

  5. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    Published
    March 7, 2017, 3:30 p.m.
    .... Pro-Tips: 1. It’s all about the MOI. In retrospect I used a way too high Cas9 MOI considering that...I didn’t do this, but to do it you would need a pretty good gel image. If you are analyzing short DNA ...cancer cell line in about 1 month, which I’d say is pretty amazing. Despite the shifting landscape of research...

  6. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    Published
    Nov. 2, 2021, 1:15 p.m.
    ... was not present (Kuhlman, SK. 2008). While theoretically this strategy should work, these vectors suffered...data and can instill greater confidence when interpreting the outcome.    References and resources References...Pillay S, Puschnik AS, Nagamine CM, Cheng F, Qiu J, Carette JE, Vandenberghe LH (2018) An Alternate Route for...

  7. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ...gku241 Cong, L., Ran, F. A., Cox, D., Lin, S., Barretto, R., Habib, N., Hsu, P. D., Wu, X., Jiang, W.,...., Terns, M. P., White, M. F., Yakunin, A. F., Garrett, R. A., Van Der Oost, J., . . . Koonin, E. V. (...Pinilla-Redondo, R., Mayo-Muñoz, D., Russel, J., Garrett, R. A., Randau, L., Sørensen, S. J., & Shah, S....

  8. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ...Off-target activities of the RNAi Correctly interpreting your data therefore necessitates careful design..., this can make the results more difficult to interpret, and a knockout/ or knockdown approach should ...validation is critical to ensuring accurate interpretation of your results. Therefore, taking advantage...

  14. Top Requested AAV of 2017: pmSyn1-EBFP-CRE

    Type
    Blog Post
    Published
    Jan. 17, 2018, 2:57 p.m.
    ...EBFP-Cre Available in serotypes AAV1, AAV5, and AAV retrograde (the most popular form), this viral vector is...with Flp-FRT. Additionally, you could use the retrograde form to specifically express Cre in neurons with...

  15. Bringing Sustainable Practices to the Lab: Innovations

    Type
    Blog Post
    Published
    March 7, 2019, 2:39 p.m.
    ...toward a more sustainable one. The math on this is pretty simple: wash your tips once and you cut your tips...distribute it to industrial and residential markets. Rethinking our approach to lab waste is something we all...

  17. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    Published
    Aug. 4, 2015, 2:30 p.m.
    ...foundation of multiple important technologies, including FRET and optogenetics. Even though GFP has been so thoroughly...characterized, it turns out this protein has a few more secrets - during a collaboration, members of Maureen Hanson...

  18. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    Published
    June 19, 2015, 3:08 p.m.
    ...subtilis and the yeast Pichia pastoris. The improved secretory capacities of these microbes make them useful ...peptide (SP) that routes the protein through the secretory pathway to improve folding and disulfide bond ...

  19. Celebrating Accomplishments in the Lab

    Type
    Blog Post
    Published
    May 8, 2014, 3:07 p.m.
    ...New Hampshire in the summer for the annual lab retreat. How do you manage work-life balance? Tom Ellis... “three generations walk[ing] together in the Arboretum and other parks.” Lab life is not all work and...

  20. Addgene is Expanding Our Viral Vector Service!

    Type
    Blog Post
    Published
    Oct. 15, 2024, 1:15 p.m.
    ...for packaging: AAV1, AAV2, AAV5, AAV8, or AAV Retrograde (AAVrg) capsids.  Please be aware that this ordering...plasmids packaged in AAV1, AAV5, AAV8, and AAV Retrograde to be 1x1013 and titers for AAV2 to be 7x1012...
Showing: 121 - 140 of 479 results