...the Gal4/UAS system. This incredibly useful, yet simple system allows you to systematically study gene ...downstream of a tissue-specific promoter sounds simpler, this modular system saves you from having to create...compartment, you could express them individually or simultaneously. Do note for control purposes that there are...

...TCSs have been identified in bacteria, and with similar optimization methods, many of these systems could...see light. Levskaya A, Chevalier AA, Tabor JJ, Simpson ZB, Lavery LA, Levy M, Davidson EA, Scouras A, ...genetic edge detection program. Tabor JJ, Salis HM, Simpson ZB, Chevalier AA, Levskaya A, Marcotte EM, Voigt...

...facilitates the reproduction of the structure in a similar way to the biological protocol, and assembly designs... young movement of sharing open hardware has a similar goal. The sharing of modules, ideas, and blueprints...Nibha Akireddy, Chew Chai, Manu Prakash, "Squid: Simplifying Quantitative Imaging Platform Development and...

...suppressor screens These types of screens are very similar to forward and reverse screens, except that they... which are worth considering. RNAi screens are similar to CRISPR, but instead of knocking out the genes...temperature to exacerbate a mutant phenotype, very similar to enhancer/suppressor screens. Selections Selections...

...promoter and ceases repression of the target gene. A simplified lac inducible system is found in many bacterial...between cell types or organisms, promoters are similarly variable. Bacterial promoters only work in prokaryotic...related species from which they were derived. Similarly, the various eukaryotic cell types (mammalian,...

...proteins they identified have little sequence similarity to previously identified Cas13a-c orthologs, ...mammalian system. Since Cas13d is functionally similar to previously discovered Cas13 enzymes - what makes...their cleavage pattern: EsCas13d produced very similar cleavage products even when guides were tiled across...

...create a genome-wide library capable of performing similarly to Cas9 screens in a compacted library. Overall...genes within a single cassette provides increased simplicity for combinatorial genetic screens, and this optimized...Penn State Protein Ladder  by: Rachel Leeson This simple, inexpensive plasmid-based ladder system is a cost-effective...

...Identifying the Issue You can’t fix an experiment by simply running it unchanged over and over again, and the...was not trying even harder to start earlier, but simply moving all my prep work to the day before, which...remember what's been added and what hasn't. Developing simple habits like these can consistently reduce errors...

...conjugate that can be activated to emit light with a simple kit, and is readily available on any number of ...ain’t broke, don’t fix it. HRP is easy to find, simple to use, not sensitive to light in its inactive ... this is an Antibody 101 post, so we'll keep it simple. If you follow the two basic guidelines below, ...

...system work? Mendenhall: The system is really quite simple but it offers improvements in efficiency and throughput...right thing to do to put these out there. It’s a simple solution. It would be silly not to share it as ...on many other labs that may be doing something similar. There may be other variants of this that are better...

...together, thus repairing the break. This is a simplified, streamlined version of this pathway and does...regions of hundreds of base pairs or more can simultaneously introduce a pair of chromosome breaks, and ...deletions) if NHEJ joins the distal ends together. Similarly, it may be possible to direct insertion of an ...

...cloning system - a Gateway compatible approach to simultaneously clone two genes-of-interest (GOI) into two ... on a single plasmid (Grefen and Blatt, 2012). Similar to traditional Gateway™ cloning, the GOI first ...the mitochondria, because cPYP binds to BoPD. A similar set of newly identified light- and dark- binder...

...organizations with similar ideas who were also thinking about reproducibility and shared similar desires to take...talented and motivated groups and individuals with similar ideas to actually do something about it. Building...

...rate as compared to a constitutive promoter. To simulate the increase in plasmid copy number during the...expression from the constitutive promoter did not. Similar levels of expression between a plasmid and the ...stabilized promoter, deoxychromoviridans titers were similar regardless of where the genes were expressed. What...

...threonine kinase with a similar 3D structure to MEK1, but Raf1 and CDK5 have less similarity. For these three...versions of MEK1, MEK2, Raf1, and CDK5 all behave similarly to their endogenous counterparts. Additionally...

...absorb the excitation light less efficiently. Similarly, decreasing the power of the excitation light ...results in a loss of fluorescence, which, in its simplest form, can be described by a mono-exponential decay...intensity decay of a fluorophore does not follow a simple mono-exponential decay (Shaner et al, 2008; Bindels...

...by daughter cells during cellular reproduction, similar to the host’s chromosome, or they can spread between...plasmids likely have undiscovered functions, many are simply genetic parasites. Cryptic plasmids tend to be ... R., Sailland, A., Auriol, P., Vogel, T. M., & Simonet, P. (2001). Laboratory-scale evidence for lightning-mediated...

... components. Using the handy website, users can simply select the components they want for each module...license.   Once you have the components you want, simply assemble them into a destination vector via Golden...summary on the Fragmid website.   Step 3 is simply a review of the assembled plasmid. It shows you...

...CEPIA (additional versions are available to simultaneously measure calcium changes in the ER, cytosol ...single fluorescent protein (FP), requiring only a simple measurement. However, single wavelength measurements...fluorescence to a red fluorescent state only by simultaneous (1) user-controlled illumination with violet...

...same target to see if the results are the same/similar, emphasizing the antibody’s specificity. Strength...from the background noise. If the sample images similarly to the negative control, that informs us that ...doi.org/10.1038/518027a Freedman LP, Cockburn IM, Simcoe TS (2015) The Economics of Reproducibility in Preclinical...