We narrowed to 186 results for: SPL;

1. Hot Plasmids: FRET-Based Biosensors

   Type

   Blog Post

   Published

   May 6, 2014, 1:07 p.m.

   ... microscope using commercially available beam splitters. In addition to imaging neurons, Griesbeck adds...

2. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

   Type

   Blog Post

   Published

   Dec. 10, 2019, 1:58 p.m.

   ...amenable to use with other bEBP/UAS pairs, and displays high dynamic range. However, the promoter in question...

3. Learning Technical Skills at a Lab Central Workshop

   Type

   Blog Post

   Published

   Feb. 20, 2018, 2:30 p.m.

   ...presentation describing the technology. Then we split into pairs to actually try out the kit itself. This...

4. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

   Type

   Blog Post

   Published

   July 27, 2017, 1:11 p.m.

   ...production of both types of HMO biosensors and displayed her findings at the Spring 2017 ACS meeting. She...

5. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

   Type

   Blog Post

   Published

   July 21, 2017, 12:48 p.m.

   ...PMID: 28704652. 6. V. Rodríguez, D. Didiano, C. Desplan, Power tools for gene expression and clonal analysis...

6. The Power Behind NGS Plasmid Validation: seqWell

   Type

   Blog Post

   Published

   April 19, 2017, 3:25 p.m.

   ...because the sample identity information is always displayed at the individual read level, and because custom...

7. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

   Type

   Blog Post

   Published

   Nov. 5, 2019, 1:28 p.m.

   ...David Liu Lab similarly described xCas9, which displayed the ability to target multiple PAM sequences (...

8. Hot Plasmids - May 2022

   Type

   Blog Post

   Published

   May 26, 2022, 1:15 p.m.

   ...work, they have found at least two variants that display increased neuronal expression in the brain while...

9. Antibodies 101: ChIP

   Type

   Blog Post

   Published

   March 10, 2022, 3:25 p.m.

   ...choose, you’ll need to reserve a small aliquot, split into two parts to (1) run a DNA gel to confirm fragment...

10. Targeted Mutagenesis with EvolvR

    Type

    Blog Post

    Published

    Feb. 21, 2019, 1:15 p.m.

    ...its native endonuclease activity degrades the displaced strand.   Figure 1: EvolvR consists of ...

11. Luminescent Imaging with Nano-lanterns

    Type

    Blog Post

    Published

    May 25, 2017, 2:30 p.m.

    ...very robust, with an eNL-based calcium sensor displaying a 500% signal change upon calcium binding. Suzuki...

12. Screening for Successful Genome Editing with Digital PCR

    Type

    Blog Post

    Published

    March 30, 2017, 2:30 p.m.

    ...below. Digital PCR Digital PCR (dPCR) involves splitting up a sample into thousands of physically isolated...

13. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type

    Blog Post

    Published

    Feb. 10, 2016, 3:30 p.m.

    ...is also common. Overlap extension PCR is used to splice together two PCR amplicons based on sequence homology...

14. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type

    Blog Post

    Published

    March 19, 2019, 1:31 p.m.

    ...the Uppalapati and Woolley lab developed a phage display technique using small scaffold proteins to identify...

15. Transferable Skills Guide: Public Speaking

    Type

    Blog Post

    Published

    April 20, 2023, 4 p.m.

    ...the nerve to do what I did. Until that panicked, split-second decision, I had always thought I had an obligation...

16. The time and cost required to make a plasmid

    Type

    Blog Post

    Published

    March 23, 2023, 1:15 p.m.

    ...same time period. For ease of calculations*, let’s split the difference and put scientific inflation at… ...

17. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type

    Blog Post

    Published

    Dec. 8, 2022, 2:15 p.m.

    ...cleavage and 2). cell toxicity. Cas7-11 does not display collateral RNA cleavage and is significantly less...

18. Choosing the B(right)est Fluorescent Protein: Photostability

    Type

    Blog Post

    Published

    June 8, 2017, 2:30 p.m.

    ...top of photobleaching, fluorescent proteins may display reversible intensity changes (Shaner et al, 2008...

19. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

    Type

    Blog Post

    Published

    Dec. 16, 2015, 3:30 p.m.

    ...SpCas9 N692A/M694A/Q695A/H698A). This new variant displays comparable on-target activity and similar or decreased...

20. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type

    Blog Post

    Published

    April 6, 2021, 12:30 p.m.

    ...chicken beta actin promoter, and rabbit beta-globin splice acceptor, contains sequence that is over 80% GC...