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  1. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ...the late 1980s, scientists even created a codon adaptation index (CAI) that is based on the codon usage ...10.1038/nrg3051 Sharp PM, Li W-H (1987) The codon adaptation index-a measure of directional synonymous codon...

  3. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...have been developed for a singular purpose (ex. adapter trimming, quality score analysis, k-mer counting...Java, R, etc.) in our pipeline. Crucially, this adaptable property gave us the freedom to use multiple open-source...

  4. Transferable Skills Guide: Creativity

    Type
    Blog Post
    Published
    Jan. 18, 2022, 2:15 p.m.
    ...your problem. In a study outlined in Originals by Adam Grant, a creativity expert at Stanford conducted... with the group (because they will fight more adamantly for their solution), selecting a devil’s advocate...

  5. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...ubiquitination substrate Ikaros (IKZF1ZF2) (B). Image adapted from Kroupova et al., 2024 under CC BY license....design and studies of protein ubiquitination and degradation.   Find the CRBNmidi plasmid here!   Kroupova...

  6. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...trafficked from the “source” to “destination”. Images adapted from Lee and Cheah et al. 2023 bioRxiv (A) and ...:GFP-iPAK4 at 3 or 6 h. Scale bar 5 µm. Images adapted from Lin et al., 2023 bioRxiv, under CC-BY-NC 4.0...

  7. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    Published
    Aug. 5, 2014, 6:51 p.m.
    ...such that prior formats are no longer supported/readable. Is the author or their host institution expected...Research Institute at Mount Sinai Hospital, Toronto, Canada where he studies protein-serine/threonine kinases...

  8. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    Published
    Feb. 22, 2017, 3:30 p.m.
    ...twist since it relies on the sequential nicking, degradation, and PCR-generation of a mutant version of the...Natarajan, Chandrasekhar, et al. "Epistasis among adaptive mutations in deer mouse hemoglobin." Science 340.6138...

  9. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    Published
    May 13, 2025, 1:15 p.m.
    ...non-integrating transgenes. Created with BioRender.com; adapted from Banasik and McCray, 2010. These episomes...Chatters, S. J., de Ridder, D., Gilmour, K. C., Adams, S., Thornhill, S. I., Parsley, K. L., Staal, F....

  10. Hot Plasmids: Summer 2024

    Type
    Blog Post
    Published
    Aug. 27, 2024, 1:15 p.m.
    ...structure of the H1.8-GFP-bound nucleosome. Images adapted from Arimura et al. 2024 under CC-BY-NC-ND 4.0 ...3D map with 3.1 Å global resolution (E). Figure adapted from Abe & Lim, 2024 under CC-BY-NC-ND 4.0 license...

  11. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...Rising τ, and Decay τ of the three sensors. Image adapted from Aggarwal et al. 2025 under a CC-BY-NC-ND 4.0...displaying characteristic PV basket cell morphology. Adapted from Furlanis, Dai, Leyva Garcia et al. 2024 bioRxiv...

  13. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    Published
    July 12, 2016, 2:30 p.m.
    ...you are working with RNAs, which are prone to degradation by the omnipresent RNase. You may want to declare...doi.org/10.1038/nbt.3026 Gurumurthy CB, Takahashi G, Wada K, Miura H, Sato M, Ohtsuka M (2016) GONAD: A Novel...

  14. Finding Your Perfect Job After University

    Type
    Blog Post
    Published
    Jan. 12, 2016, 3:30 p.m.
    ... culture, and also needed to be resourceful and adapt. By the end of my three months, I fully understood...companies which have more bureaucracy and cannot be as adaptive. I was also surprised to learn that I enjoy ...

  15. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ... editing methods, other Cas proteins have been adapted for use in C. elegans. In 2017, the Korswagen lab...doi.org/10.17912/DDVH-BG64 Tzur YB, Friedland AE, Nadarajan S, Church GM, Calarco JA, Colaiácovo MP (2013)...

  18. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ... the balance of biogenesis and mitochondrial degradation in vitro and in vivo. Merged green and red ...long life-cycle of a protein from expression to degradation. Chemical approaches may require expensive peptides...

  19. New Coomassie Protocol Video

    Type
    Blog Post
    Published
    Oct. 31, 2023, 1:15 p.m.
    ...Antibodies."  Hot tip! This protocol can easily be adapted for other types of proteins.    If this video ...

  20. CRISPR 101 eBook...en español

    Type
    Blog Post
    Published
    Jan. 18, 2024, 2:15 p.m.
    ...está disponible en español! Esta traducción fue regalada a Addgene por Daniel Mejías Sánchez, ¡y no podríamos...
Showing: 121 - 140 of 292 results