...Having seen CRISPR’s success in basic research, researchers are eager to apply it in a clinical setting...Find CRISPR Plasmids for Your Research Find Validated gRNAs for Your Research ...preclinical and clinical work. Find Plasmids for Disease Research on Our Special Collections Pages Duchenne muscular... around 30 years of age. Despite the amount of research conducted on DMD, there is still no good treatment...it difficult to deliver via gene therapy, so researchers have set their sights on another approach. Since...oligonucleotide treatment comes with many challenges, researchers have begun to explore genome editing approaches...CRISPR-mediated exon skipping approach in mice, DMD researchers are very excited. This approach may also be applicable...

...skills required Research skills The ability to think critically and carry out in-depth research is applicable...career, do your research Once you have identified possible careers, the next step is to research what each ...Epidemiologist Scientific Recruitment Meteorologist Research and Development Healthcare Consulting Lab Manager...Manager Patent Lawyer Science Market Research Statistician   Attention to detail Patience, accuracy...include: Science Reporter Scientific Illustrator Research and Development Scientific Advisor Lab Manager...Before you begin to apply for a job, it is useful to search online job websites to see what requirements or...Once you’ve assessed your skill set and done your research, it’s time to start sending out applications. ...

... CRISPR technologies available to the academic research community. At Addgene, we’ve helped distribute...provide ready-to-use CRISPR lentiviral preps to researchers across the globe. Why provide CRISPR as virus...proceed to use it for experiments.” - Alex Chavez, researcher at the Wyss Institute for Biologically Inspired...can make lentivirus production frustrating - a researcher can complete an entire lentiviral production ...receiving a pooled gRNA library in plasmid format, a researcher must amplify the library, use next-generation...reagents from Addgene can greatly expedite a researcher's ability to make discoveries." If you ever run...plasmids to create your own lentivirus stocks. Our research scientists have provided their standard lentivirus...

...focused on translational research is encouraging. Patients are key to the research, but they are also the...observations regarding what makes this group of researchers special: 1. Stem cell scientists care deeply ...Listen to Our Podcast Interview with Stem Cell Researcher Darrell Kotton 2. Stem cell scientists care about... on spinal cord injuries; Jessica Young shared research on stem cells and Alzheimer's Disease; and Kevin...the reason this research is happening. Stem cells scientists have certainly not lost site of that. 3. Stem...acquisition is not a rate-limiting step in stem cell research. People told me they couldn't have done their ... to helping the stem cell community with their research, I think I can speak for Addgene when I say that...

...Information DatabaseResearcher: Amos BairochViral Vectors 101: Systemic CapsidsResearchers: Cynthia Arokiaraj...work with Addgenie writers to help share their research, tools, and knowledge. Guest authors The Codon... Benedict Diederich Course-Based Undergraduate Research Experiences (CUREs)Author: Jason PellettieriNew...NEBGCE4All: Making Genetic Code Expansion AccessibleResearcher: Ryan Mehl; Andy KarplusIBEX Knowledgebase...Knowledgebase: A Data Resource for Multiplex Tissue ImagingResearchers: Andrea Radtke, Ziv YanevCellosaurus: A Cell...ShayMultifunctional Neuronal Fibers: Modulation from Gut to BrainResearchers: Atharva Sahasrabudhe; Polina Aniveeka   A...blog this year! Your contributions have helped researchers around the globe find and share knowledge, resources...

...statement: Accelerate research and discovery by improving access to useful research materials and information...post was contributed by Kyle Hooper at Promega. Researchers have been sharing plasmids ever since there were...examples of technologies that can accelerate your research. The creation of NanoLuc® Luciferase from a rather... or above 600 nm wavelengths should be used. Researchers often use bioluminescence to overcome these issues...brightness of NLuc has caught the attention of researchers wanting to perform whole-animal imaging, however...leads to spurious activation of the FRET sensor. Researchers have sought to alleviate many of these challenges...also activate the optogenetic accuators. The researchers took a FRET reporter and adapted it into a BRET...

...Plasmids from:  March (Q1) | June (Q2) | September 2016 (Q3) | December 2016 (Q3) MARCH 2016Enhancer activity... much literature searching, DNA synthesis, and plasmid assembly. Luckily, researchers from Douglas Densmore... states. Alexander Stark and colleagues at the Research Institute of Molecular Pathology have developed... library     CRISPR pooled libraries enable researchers to screen the entire genome for genes that regulate...newly available PRESTO-TANGO kit which allows researchers to interrogate the druggable human GPCR-ome using...utility of this platform for drug discovery. Researchers can use plasmids from the PRESTO-TANGO kit directly...-disciplinary Integration of Design Automation Research lab Moduclar Cloning (CIDAR MoClo) Parts Kit. ...

...various light-controlled CRISPR tools available to researchers - some readily found at Addgene. Shining light...position to activate gene transcription. The researchers tested a variety of combinations to optimize ...pattern during blue light exposure (470nm), the researchers showed that expression of the human ASCL1 gene...cells to induce expression of human IL1RN, the researchers saw an 11-fold increase in mRNA levels after ...cycled on-off-on. Using a slit photomask, the researchers also demonstrated the ability to spatially control...-mediated CRISPR labeling to study chromatin architecture (Hoffman et al., 2020). Fluorescent dCas9 proteins...can potentially alter the genome and chromatin architecture, it is useful to regulate the time in which ...

...Addgene to accelerate their research. Read on to learn how these researchers plan to use plasmids from ...Genomics Institute to provide plasmid grants to researchers working on rare diseases through the BeHEARD ...from Addgene to advance their research on diseases of glycosylation, arginine mutagenesis, and neural development...She leads the Glycoimmunology group based at the Research Unit on Applied Molecular Biosciences (UCIBIO)... (APCDG) and an international platform for CDG research, named the CDG&Allies – Professionals and Patient... Colorado, USA. The primary focus of his lab’s research is the mechanisms by which proteins assemble into... a professor at the University of Buffalo. His research focuses on understanding the structural determinants...

...whole-genome sequencing becomes more relevant (Garcillán-Barcia et al., 2023). In this post, we’ve grouped...https://doi.org/10.1016/j.cell.2024.01.039 Garcillán-Barcia, M. P., Redondo-Salvo, S., & de la Cruz, F...experimental tools. They certainly are very handy in research, but we’re really borrowing these tools from Mother...think of bacteria when we think of plasmids, both Archaea and Eukaryota can also harbor these circular DNA...plasmids can be dangerous, they also provide researchers with new means to deliver DNA and other molecules...tend to be small and poorly studied, but some researchers have suggested they may have relevance to human...natural plasmids and their functions has enabled researchers to develop and deposit the many plasmid tools...

...Antibiotic Resistance Genes By Marcy Patrick Plasmids 101: Yeast Vectors By Marcy Patrick CRISPR-Cas9 FAQs...for your research during this holiday season and we look forward to seeing what awesome research you and... offering to share their stories – about their research, their job hunts, and their tips for experiments...you have any questions or if you have a topic, researcher, or technology you would like us to feature in...looking for guest bloggers who want to share their research with the community, so email us at [email protected] Morgan Plasmids 101: Mammalian Vectors By Marcy Patrick Will You Be My Mentor? Finding and Asking...

...increasingly popular in fundamental and applied research since their first use in the early 90’s to genetically...inefficient at infecting non-dividing cells and so researchers set out to create a better, safer system. Unlike...than the first one and can be used routinely in a research laboratory. The risk of generating RCL is low ...technique, there’s always a small chance that a researcher could infect him or herself with the oncogenic... generation lentiviral vectors can be used for research and for clinical purposes, however improving the...safety of these vectors is still an active area of research due to the possibility that mutation or recombination...lentiviruses with human tropism can still infect the researcher and insert into her/his genome - this is a biosafety...

... large-scale profiling of many GPCRs, Mikel Garcia-Marcos’ lab recently developed a collection of BRET-based.... E., Zhang, H., Layne, M. D., Wu, J. C., & Garcia-Marcos, M. (2024). Direct interrogation of context-...biosensors kit Antibodies for neurodegeneration research   Optimized prime editing in dicots By Emily...New recombinant antibodies for neurodegeneration research   By Ashley Waldron We recently added two antibodies...against important targets for neurodegeneration research: Anti-MAPT [AT8] and Anti-APP [6E10-A5]. Both ...hope these will become valuable tools for your research! If they do (or if they don't!), please consider...consider sharing your experience with other researchers by contributing to the Addgene Antibody Data Hub.  ...

...inhibition of action potential generation. Archaerhodopsin (Arch) is activated at a similar spectrum as HR... the first time in the history of neuroscience research we have a technology that allows us to show causality...discarded but clearly we are in an era of neuroscience research where optogenetics will be a standard tool used...the National Institutes of Drug Abuse Intramural Research Program (NIH-NIDA IRP), with the stated goal of...they can be used is to consult the many primary research papers that have been published at this point....through hydrogen ion efflux; evidence suggests that Arch may more effectively silence neurons than HR. Delivering...construct using stereotaxic surgery is a neuroscience research technique often used beyond just optogenetics....

...10 blog posts from the year. 10. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage...plasmids every quarter. The “hot plasmids” from last March were quite popular and represent the diversity of...academic job interview including defining your research vision, organizing your vision into a chalk talk...many model organisms people are using for their research. In this blog post, we’ve covered five model organisms...yeast, zebrafish, and worm. 4. Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics... technique in molecular biology and scientific research. Get an overview of these methods in this blog...fantastic year and happy reading. Best wishes on your research in 2020! Additional resources on the Addgene ...

...contributed by guest bloggers Marcelle Tuttle and Alex Chavez, researchers at the Wyss Institute for Biologically... thanks to our guest bloggers Marcelle Tuttle and Alex Chavez! Marcelle Tuttle is a medical student at...domain (Fig 1). Two ways to use CRISPRa for your research CRISPRa for specific gene targets CRISPR activation...scale of activation allows for exploratory-based research as well. For example, CRISPRa can be used to find... at Tufts University and former researcher at the Wyss Institute for Biologically Inspired Engineering...Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H,...Zetsche B, Volz SE, Zhang F (2015) A split-Cas9 architecture for inducible genome editing and transcription...

...  Easily search for plasmids (and more!) The main goal for the homepage was to improve search and browse...redesign, the new search bar is now front and center, with a clear call to action - Search! Along with this...It became clear to us that we needed to improve search and navigation, while also updating brand messaging...Community, sharing, and fun Along with improving search and navigation, we also wanted to emphasize the...the Addgene blog Learn more about Addgene's new search tool Read more about other features of Addgene'...website Resources on Addgene.org Try out the new search on Addgene's homepage ...

...in full-length publications. As early career researchers, we wondered how we could make the insights gained...around reading through entire papers, perhaps you search google images for specific experimental results...These data are valuable but remain hidden from research papers as they are not included in traditional...study with multiple patients was published, Zika researcher Christa Osuna collated Zika case studies, making... the NIH and Wellcome Trust now accept interim research products (pre-registration, preprints, data, software...micropublications) in grant proposals. ReFigures are one such research product which can convince reviewers of your expertise...recommend sharing ReFigures on your Linkedin and ResearchGate profile. To see how to create ReFigures in a...

...your favorite search term, i.e. “biology + masters.” For lab-based roles, look for “research associate,”...have masters by using the search term “master’s degree.” Use the LinkedIn search filters to limit results...postings  Look at master’s-level job postings on a job search website like Indeed. Do the job descriptions sound...manuscript and grant preparation, and lead your own research project.  3. Do you prefer being a generalist?...variety of projects and in different fields of research is one of the best parts of my career with a master...master’s. While it's possible to change fields of research with a PhD, a master’s is less specialized so ...

...mechanism of incorporation is not well understood, researchers have been using AAV vectors for gene editing ...iPSCs from ~0.2% to ~1.5%. In more recent work, researchers have combined AAVs with CRISPR systems. Ohmori...an AAV’s ~4.5 kB packaging capacity. Luckily, researchers have devised methods to deliver CRISPR components...delivery method frees up space in the AAV allowing a researcher to fill it with the repair template. Bok and ...hematopoietic stem cells respectively. Future researchers could apply this technique to efficiently deliver... continue to use and develop AAVs for your own research and encourage to deposit any new AAV tools you...Cancer Center, Georgetown School of Medicine. His research currently focuses on determining the mechanisms...