We narrowed to 247 results for: construct

...mice pre-CRISPR was very arduous. First, you’d construct a plasmid with your desired changes, long homology...template, you'd use ivTRT to create the ssDNA construct as described in Figure 2.  You can also use Addgene...

...The aim of these meetings is to give honest, constructive feedback, set tangible research and career goals...

...and tracking cells that express both the dCas9 construct and the fluorescently-labeled gRNA multiplex. ...

...hypothesis that the lower expression levels of SunTag constructs would avoid negative effects on cell physiology...

...community even further. If you have a great new construct you’d like to share with the scientific community...

...intellectual property rights and handling your constructs once they reach you. As with all of our resources...

...subcellular localization studies or make a reporter construct. Fluorescent proteins (FPs) are also the foundation...

...through multicolor labeling and FRET. Specialty constructs for biosensing. Over 300 plasmid backbones ...

...library within cells and aimed for 300 cells per construct, but in some biological systems one “might need...

...the research directions of the lab, gives you constructive feedback on your research, and teaches you how...cover letters, fellowships, etc. Second, get constructive feedback from experts. If someone crosses out...

...compelled to be part of that.” He’s deposited AAV constructs for gene delivery. In 2018, he interviewed Addgene...

...with biotin as an affinity tag. This ‘Caspex’ construct is guided by a pre-defined sgRNA sequence to a...

...efficiency was not affected by CellTagging. By constructing lineage trees and comparing them with the gene...

...protein that they are fused to. The researchers constructed 22 different mTFP1 fusions to both the C- and...

...cycles or low fidelity Taq DNA polymerase, the construction of specific sequences for base pair overhangs...

...to the community. We now offer AAV9 for 2 more constructs from Christopher Harvey’s lab. These plasmids...

...PMCID: PMC2134509. Cohen, Stanley N. et al. “Construction of Biologically Functional Bacterial Plasmids...

...pluripotent stem cell (iPS) technology and CRISPR-Cas9 constructs from Addgene to gene-edit mutations into XP genes...

...to drive the selection/reporter parts of each construct. This new tool kit allows the rapid generation...

...vector-insert joining for recombinant plasmid construction (restriction cloning), adaptor ligation for ...for next generation sequencing (NGS) library construction, and circularization of dsDNA (6). Less commonly...