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We narrowed to 249 results for: construct

Showing: 121 - 140 of 249 results
  1. Hot Plasmids: Fall 2025

    Type
    Blog Post
    Published
    Nov. 11, 2025, 2:14 p.m.
    ... this paper, a large menu of tool and control constructs is available to choose from! Find plasmids to...Experimental procedure for xenograft experiments. B) Constructs for tet-inducible CRISPRa-AAV engineering. Adapted...
  2. Exploring Applications of the Bioluminescent HiBiT Tag

    Type
    Blog Post
    Published
    Oct. 2, 2025, 1:15 p.m.
    ...Promega Plasmid Collection that express similar constructs available at Addgene. Read the original paper...et al., 2025). They designed HiBiT-tagged TP53 constructs, one with an R175H mutation to match a frequent...
  3. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ...mice pre-CRISPR was very arduous. First, you’d construct a plasmid with your desired changes, long homology...template, you'd use ivTRT to create the ssDNA construct as described in Figure 2.  You can also use Addgene...
  4. SunTag and Fluorescent Imaging

    Type
    Blog Post
    Published
    March 28, 2017, 2:30 p.m.
    ...hypothesis that the lower expression levels of SunTag constructs would avoid negative effects on cell physiology...
  5. What's New in CRISPR - March 2020

    Type
    Blog Post
    Published
    March 10, 2020, 1:15 p.m.
    ...and tracking cells that express both the dCas9 construct and the fluorescently-labeled gRNA multiplex. ...
  6. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    Published
    Aug. 4, 2015, 2:30 p.m.
    ...subcellular localization studies or make a reporter construct. Fluorescent proteins (FPs) are also the foundation...
  7. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    Published
    Oct. 9, 2018, 12:21 p.m.
    ...the research directions of the lab, gives you constructive feedback on your research, and teaches you how...cover letters, fellowships, etc. Second, get constructive feedback from experts. If someone crosses out...
  8. Meet the New Addgene Board Members!

    Type
    Blog Post
    Published
    April 28, 2020, 1:14 p.m.
    ...compelled to be part of that.” He’s deposited AAV constructs for gene delivery. In 2018, he interviewed Addgene...
Showing: 121 - 140 of 249 results