We narrowed to 245 results for: construct

1. SunTag and Fluorescent Imaging

   Type

   Blog Post

   Published

   March 28, 2017, 2:30 p.m.

   ...hypothesis that the lower expression levels of SunTag constructs would avoid negative effects on cell physiology...

2. What's New in CRISPR - March 2020

   Type

   Blog Post

   Published

   March 10, 2020, 1:15 p.m.

   ...and tracking cells that express both the dCas9 construct and the fluorescently-labeled gRNA multiplex. ...

3. Addgene at the ASM Microbe 2016 Conference

   Type

   Blog Post

   Published

   July 7, 2016, 2:30 p.m.

   ...community even further. If you have a great new construct you’d like to share with the scientific community...

4. A Better Way to Get Customer Support: The Help Center

   Type

   Blog Post

   Published

   Dec. 10, 2015, 3:30 p.m.

   ...intellectual property rights and handling your constructs once they reach you. As with all of our resources...

5. Seeing Red: Simple GFP Photoconversion

   Type

   Blog Post

   Published

   Aug. 4, 2015, 2:30 p.m.

   ...subcellular localization studies or make a reporter construct. Fluorescent proteins (FPs) are also the foundation...

6. The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

   Type

   Blog Post

   Published

   July 29, 2014, 6:16 p.m.

   ...through multicolor labeling and FRET. Specialty constructs for biosensing. Over 300 plasmid backbones ...

7. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

   Type

   Blog Post

   Published

   Feb. 20, 2014, 4:57 p.m.

   ...library within cells and aimed for 300 cells per construct, but in some biological systems one “might need...

8. 9 tips for a successful postdoctoral experience

   Type

   Blog Post

   Published

   Oct. 9, 2018, 12:21 p.m.

   ...the research directions of the lab, gives you constructive feedback on your research, and teaches you how...cover letters, fellowships, etc. Second, get constructive feedback from experts. If someone crosses out...

9. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

   Type

   Blog Post

   Published

   June 27, 2018, 1:16 p.m.

   ...with biotin as an affinity tag. This ‘Caspex’ construct is guided by a pre-defined sgRNA sequence to a...

10. Single-cell tracking of lineage and identity with CellTag

    Type

    Blog Post

    Published

    May 9, 2019, 1:15 p.m.

    ...efficiency was not affected by CellTagging. By constructing lineage trees and comparing them with the gene...

11. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type

    Blog Post

    Published

    April 25, 2019, 3:01 p.m.

    ...protein that they are fused to. The researchers constructed 22 different mTFP1 fusions to both the C- and...

12. Meet the New Addgene Board Members!

    Type

    Blog Post

    Published

    April 28, 2020, 1:14 p.m.

    ...compelled to be part of that.” He’s deposited AAV constructs for gene delivery. In 2018, he interviewed Addgene...

13. New and Upcoming Viral Vectors - December 2019

    Type

    Blog Post

    Published

    Dec. 19, 2019, 2:40 p.m.

    ...to the community. We now offer AAV9 for 2 more constructs from Christopher Harvey’s lab. These plasmids...

14. FastCloning: A Newer, Simpler Cloning Technique

    Type

    Blog Post

    Published

    Nov. 1, 2016, 2:30 p.m.

    ...cycles or low fidelity Taq DNA polymerase, the construction of specific sequences for base pair overhangs...

15. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type

    Blog Post

    Published

    Oct. 29, 2015, 2:30 p.m.

    ...PMCID: PMC2134509. Cohen, Stanley N. et al. “Construction of Biologically Functional Bacterial Plasmids...

16. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type

    Blog Post

    Published

    Sept. 15, 2015, 2:30 p.m.

    ...pluripotent stem cell (iPS) technology and CRISPR-Cas9 constructs from Addgene to gene-edit mutations into XP genes...

17. Sleeping Beauty Awakens for Genome Engineering

    Type

    Blog Post

    Published

    June 30, 2015, 2 p.m.

    ...to drive the selection/reporter parts of each construct. This new tool kit allows the rapid generation...

18. When Fidelity Matters: A frank discussion about ligase fidelity

    Type

    Blog Post

    Published

    March 6, 2018, 1:49 p.m.

    ...vector-insert joining for recombinant plasmid construction (restriction cloning), adaptor ligation for ...for next generation sequencing (NGS) library construction, and circularization of dsDNA (6). Less commonly...

19. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type

    Blog Post

    Published

    Aug. 15, 2017, 1:24 p.m.

    ... mice expressing fluorescent protein reporter constructs in the immune lineage. By specifically labeling... efficiently in two-photon microscopy and new constructs have facilitated the creation of mouse lines ...

20. "Build Your Plasmid": The Game - Play to Teach

    Type

    Blog Post

    Published

    July 17, 2018, 12:23 p.m.

    ...justifying each single element used to build the construct and explain why they selected a certain target...