We narrowed to 180 results for: dap

1. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

   Type

   Blog Post

   Published

   Dec. 14, 2021, 2:15 p.m.

   ...methylation, leaving the target CpG unmethylated. Adapted from Sapozhnikov and Szyf 2021.   Let me leave...

2. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

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   Blog Post

   Published

   Aug. 24, 2021, 1:15 p.m.

   ...indirect ELISA (more details below) can also be adapted to a competitive format. Figure 2: Overview...

3. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

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   Blog Post

   Published

   April 6, 2021, 12:30 p.m.

   ...library. This PCR step also adds the necessary adapter sequences for Illumina sequencing as well as unique...

4. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

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   Blog Post

   Published

   Aug. 20, 2019, 12:31 p.m.

   ...locomotion. Mice may need to be given some time to adapt to a testing apparatus. General stress levels of...

5. Open Resources and Plasmid Tools For Studying C. elegans

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   Blog Post

   Published

   July 18, 2019, 12:55 p.m.

   ...Degradation System (AID) The Dernberg lab in 2015 adapted the AID system discovered in plants to conditionally...

6. Mouse Modeling, Part 1: Genetically Engineered Mice

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   Blog Post

   Published

   July 11, 2019, 1:26 p.m.

   ...mouse models provide researchers with useful and adaptable tools to perform basic and preclinical research...

7. Adenoviral Vector Production and Troubleshooting

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   Blog Post

   Published

   June 14, 2018, 11:44 a.m.

   ...which are essential for assembly of the virion. Adapted from Lee et al., Adenovirus-mediated gene delivery...

8. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

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   Blog Post

   Published

   May 15, 2018, 12:43 p.m.

   ...protein-protein interactions. We’re excited to see how you adapt similar FLINC biosensors to work in your lab. Find...

9. Twenty Years of Addgene Sharing: CRISPR

   Type

   Blog Post

   Published

   April 18, 2024, 1:15 p.m.

   ...our requestors. However, we can again see quick adaptation and steady use of all types of libraries, once...

10. Plasmids 101: Degron Tags

    Type

    Blog Post

    Published

    March 14, 2024, 1:15 p.m.

    ...to ubiquitination and proteasomal degradation. Adapted from Nabet et al., Chemical Biology, 2018.  ...

11. Viral Vectors 101: Voltage Indicators

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    Blog Post

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    Jan. 30, 2024, 2:15 p.m.

    ...of the previously described systems. Several adaptations of this method have been to tag, cage, uncage...

12. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type

    Blog Post

    Published

    Dec. 10, 2020, 1 p.m.

    ...through error-free homologous recombination (HR). Adapted from an image provided by Manuel Gonçalves; made...

13. Truncated gRNAs for Regulating Gene Expression

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    Blog Post

    Published

    Jan. 10, 2017, 3:37 p.m.

    ...interacts with a ≤15nt gRNA that contains an RNA adapter capable of recruiting a transcriptional activator...

14. Plasmids 101: Knockout/Knock-In Plasmids

    Type

    Blog Post

    Published

    Dec. 1, 2016, 3:30 p.m.

    ...them by depositing with Addgene! Figures were adapted from: Hall B, A Limaye, & AB Kulkarni. (2009) "...

15. Plasmids 101: 5 factors to help you choose the right cloning method

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    Blog Post

    Published

    Aug. 21, 2018, 12:31 p.m.

    ...Gateway cloning protocol is fairly flexible and adaptable to cloning different types of DNA, such as cDNA...

16. Plasmids 101: Protein Expression

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    Blog Post

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    June 7, 2018, 1:17 p.m.

    ... grown in well-defined media and can be easily adapted to fermentation allowing for large-scale, stable...

17. Plasmids 101: Optimizing Plasmid Yields

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    Blog Post

    Published

    May 26, 2016, 2:30 p.m.

    ...strains are not good for cloning Bacterial strains adapted for protein purification use the cell’s resources...

18. Plasmids 101: Multicistronic Vectors

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    Blog Post

    Published

    Sept. 9, 2014, 8:20 p.m.

    ...disadvantages of the IRES element, scientists have adapted "self-cleaving" 2A peptides into their muliticistronic...

19. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type

    Blog Post

    Published

    Dec. 12, 2013, 3:35 p.m.

    ...medicine. The parts we have right now are well adapted to work in prokaryotic bacteria, not in fungal ...

20. An Introduction to Adenovirus

    Type

    Blog Post

    Published

    Oct. 3, 2023, 1:15 p.m.

    ...cellular events, all recruit strong innate and adaptive immune responses that inactivate viral genomes...