We narrowed to 687 results for: eng

... while engineering viruses Acr genes can be used as positive selectable markers in engineering viruses... was used to replace genes in the difficult-to-engineer Sulfolobus islandicus rod-shaped virus 2 (Mayo-Muñoz...that have the gene deletion could replicate when challenged with the S. islandicus CRISPR-Cas system. 5. ... proteins are small enough that they could be engineered to “armor” therapeutic phages to protect them...He F, Jørgensen J, Madsen P, Bhoobalan-Chitty Y, Peng X (2018) Anti-CRISPR-Based and CRISPR-Based Genome...

... cargo (DNA or RNA) to target cells has been challenging. A variety of vector delivery technologies have...adenovirus for productive infection. AAV vectors are engineered to provide additional safety benefits: they lack...treatment appears to be both safe and effective. Challenges for AAV gene therapy Although AAV-based gene ...promising, scientists are working to overcome key challenges to their efficacy:  Pre-existing immunity: Many...with gene therapy in childhood blindness." New England Journal of Medicine 372.20 (2015): 1920-1926. PubMed...PubMed PMID: 12756417. 5. Chan, Ken Y., et al. "Engineered AAVs for efficient noninvasive gene delivery ...

...) signal. While many studies focus on promoter strength as a determinant of gene expression levels, the...multiple adenine (A) nucleotides to the tail of a messenger RNA transcript. The purpose and mechanism of polyadenylation... terminators, such as T7 and rrnB, as well as engineered high-efficiency terminators such as T0. Rho-independent...annotation for 90 bacterial elements. By Ppgardne at English Wikipedia, CC BY-SA 3.0, https://commons.wikimedia.org...initiating the desired cleavage event, although some engineered terminators come close (>95%). For most purposes...detailed analysis of yeast terminators of varying strength. A discussion of the effects of various terminators...

...advs.202202957 Yuan, T., Yan, N., Fei, T., Zheng, J., Meng, J., Li, N., Liu, J., Zhang, H., Xie, L., Ying...researchers in the Keith Joung lab, who were engineering an ABE to reduce its tendency to make off-target...they were using consisted of Cas9 fused to an engineered adenosine deaminase capable of chemically converting...sites. To explore this further, the team started engineering a CBE to be worse at its original job. They ...causing double-strand breaks (DSBs). The Fei-Long Meng Lab did in-depth profiling to compare editing outcomes..., Wang, B., Luo, Y., Cai, Y., Liu, X., Xu, Z., … Meng, F.-L. (2024). C-to-G editing generates double-strand...

...Fujita and Fujii, 2013). They call this method engineered DNA-binding molecule-mediated chromatin immunoprecipitation...immunoprecipitation (enChIP) because any engineered DNA-binding molecule, such as Zinc-finger or TAL proteins...the "in cell" form of this method, in which an engineered DNA-binding molecule such as the CRISPR complex... and identification of associated proteins by engineered DNA-binding molecule-mediated chromatin immunoprecipitation...Identification of telomere-associated molecules by engineered DNA-binding molecule-mediated chromatin immunoprecipitation...genomic regions." Journal of bioscience and bioengineering 108.5 (2009): 446-449. PubMed PMID: 19804873...

...biological engineering. 2010;4(1):1. Pubmed PMID: 20205762. PubMed Central PMCID: PMC2822740. 2. Engler C, Kandzia...Knight TF. Engineering BioBrick vectors from BioBrick parts. Journal of biological engineering. 2008;2:5...neighboring fragments require compatible cohesive ends Engler et al., 2008 Gibson Cloning Gibson assembly method...MXS-chaining method have used the technique to engineer various constructs with applications in flow cytometry...

...to the challenge. Find the PHP Plasmids Here! Their Solution: Use the CREATE method to engineer two new...3) they have limited toxicity. However, a key challenge of using AAVs for neuroscience research is the...AAV.PHP.S targets the PNS since it was actually engineered to target the CNS. How well do these AAVs transduce...takeaways: Two new AAV capsids (figure 1) were engineered with the CREATE method (figure 2) to allow for...References 1. Chan, KY, Jang MJ, Yoo BB, et al. Engineered AAVs for efficient noninvasive gene delivery ...

...Aequorea victoria. It has a fluorescent emission wavelength in the green portion of the visible spectrum ...study of dynamic, physiological processes. Re-engineering GFP to increase its range of color and application...its sequence was elucidated, scientists began engineering new versions of GFP through mutagenesis in order...iterations of fluorophores are constantly being engineered. Table 1 lists a few common fluorescent proteins...component (cyan derivatives) Y66H Blue-shifts the wavelength (blue derivatives) Y145F Increases quantum ...at high concentrations T203Y Red-shifts the wavelength (yellow derivatives) A206K Interferes with ...

...alongside some creative (and significant!) protein engineering. They combined Prime editing with an integrase...right integrase for this new approach proved a challenge. The old standby of CRE/flp didn’t work – it turns...potential applications. But they weren’t done engineering quite yet – surely, they thought, the integration...optimized the specific attachment sites via sequence engineering, enabling even more efficient integration activity...system that could integrate genes up to 36 kb in length, with an integration rate of 10-55% - the PASTE...: Adding Precision and Flexibility to CRISPR Engineering CRISPR 101: RNA Editing With Cas13  CRISPR Cheat...

...Nguyen NT, Zheng Z, Gonzales APW, Li Z, Peterson RT, Yeh J-RJ, Aryee MJ, Joung JK (2015) Engineered CRISPR-Cas9...-associated virus (AAV).  At 984 amino acids in length, Cas9 from Campylobacter jejuni (CjCas9) is even...first non-Cas9 CRISPR protein adapted for genome engineering was Cas12a (formerly Cpf1), a nuclease that generates...Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants. Science 368:290–296 . https... genome engineering...

...phage-assisted continuous evolution and protein engineering to generate seven new prime editors, PE6a–g, ...(2023). Phage-assisted evolution and protein engineering yield compact, efficient prime editors. Cell,...delivery and reduce off-target effects of gene therapy, Feng Zhang’s lab has developed a system called DIRECTED...local proteome, but some applications have been challenging or inaccessible. The Ting lab recently made two... increase the utility of this technique.  By engineering a light-sensitive LOV domain into the TurboID...organisms. Lee, S.Y., Cheah, J.S., et al. (2023). Engineered allostery in light-regulated LOV-Turbo enables...

... pairs, long-read sequencing can read sequence lengths in the thousands or even millions of base pairs...BioRender.com. Nanopore can provide longer read lengths of 10 kb to 100 kb (or 4 Mb in ultra-long read ... up the pace Our QC team constantly faces the challenge of keeping up with the large amount of QC analysis...auto-align process to match sequence results to full-length predicted sequences submitted by depositors. A ...100% nucleotide match to base identity and plasmid length. Any mismatch or indel flags the sequence for manual... sequence for a 100% match to base identity and length, but disregards the specified discrepancies. Any...

...the Wyss Institute for Biologically Inspired Engineering. CRISPR/Cas9 is an enormously plastic tool and...that can reassemble when exposed to specific UV wavelengths or chemicals (Polstein and Gersbach, 2015; Zetsche...activator by simply providing a new gRNA rather than engineering an entirely new protein. This makes it much cheaper...the Wyss Institute for Biologically Inspired Engineering.     Alejandro (Alex) Chavez is a member of the...Church GM (2015) Unraveling CRISPR-Cas9 genome engineering parameters via a library-on-library approach....Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Nature 517:583–588 . https...

...-targeting cutting as full-length gRNAs Lastly, the effects of guide length on off-target cuts was explored...17-18 bp) have less off-target cutting than full-length guides (19-20bp)?  Small-scale studies (Tsai et...explored using full-length (19-20 bp) or short (17-18 bp) guides. When comparing the toxicity of guides...short guides had reduced toxicity compared to full-length guides. Reduced toxicity of short guides could ...28474669. Pubmed Central PMCID: PMC5424143. 2. Tsai, Shengdar Q., et al. "GUIDE-seq enables genome-wide profiling...

...one of those stories. Viral vectors are viruses engineered to be safely used to deliver a genetic payload...depending on their serotype, are particularly challenging. Their efficacy in any given system can be affected...significant time and removes a variable from the lengthy process of validating and using an AAV in a specific...activated, respectively, by substrates, light, or engineered ligands (Figure 2). Biosensors, which include...accessible. The supporting data and community engagement required to do this drove Addgene to develop ...partnerships, and new platforms, from our early engagement with UPenn to our launch of the redesigned Data...

...unfamiliar, scFvs are often the building blocks for engineering proteins. So let’s get acquainted with scFvs....plasmid in bacteria (Ahmed et al., 2012). One challenge of bacterial expression is the proper formation... antibodies to be used as building blocks for engineered proteins. This flexibility is largely due to ...When these molecules, called Bispecific T-cell engagers (BiTE®s), bind CD3 on T cells and a tumor-specific...minimal systemic side effects. scFvs are part of engineered chimeric antigen receptors (CARs). CARs are a...Bruccoleri RE, Haber E, Crea R (1988) Protein engineering of antibody binding sites: recovery of specific...

...mediated transduction and expression of the full-length transgene in vitro and in vivo (Allocca et al.,..., the fragments reassemble and produce the full-length gene. Below are overviews and drawbacks of four...and B of the transgene to reconstitute the full-length transgene (Chamberlain et al., 2016). It’s generally...halves of the transgene will reconstitute the full-length transgene.  Drawbacks:  Trans-splicing occurs...sequence, the 3’ half of the transgene. The full length gene is reconstituted by either the overlapping...10.1038/mt.2013.184 Lai Y, Yue Y, Liu M, Ghosh A, Engelhardt JF, Chamberlain JS, Duan D (2005) Efficient in...

...the best ways to make you and your presentations engaging and memorable. If you read my story above about... results, striking images, creative ideas, and engaging stories as you encounter or experience them. Drop...everything I allow 1 slide for every 1 minute of talk length, and then I cut a few more slides. Do not run over...to be conversational - the audience should feel engaged and not like they’re sitting through a pre-recorded...experience you can experiment with other ways to engage the audience. Take an informal poll by asking for...from the speaker, but actually makes for a more engaging session. The best speakers use no notes and can...

...all cases, the result can be quantified by the strength of the signal in comparison to a control sample...in the synthesis of DNA strands of all possible lengths because extension is terminated upon the incorporation...become obsolete. For instance, the popular genome-engineering tool, CRISPR, may one day make techniques used...new prenatal test for sickle-cell anemia." New England Journal of Medicine 307.1 (1982): 30-32. PubMed...Andrew Cho, and Ashok B. Kulkarni. "Overview: engineering transgenic constructs and mice." Current Protocols...254.5028 (1991): 59-67. PubMed PMID: 1925562. 10. Feng, Yanxiao, et al. "Nanopore-based fourth-generation...

...Precision genome editing faces the challenges above and more. One chief challenge is upregulating HDR in mature...complex oligonucleotide treatment comes with many challenges, researchers have begun to explore genome editing...necrosis compared to unedited animals. In grip strength, specific force, and other muscle tests, muscle... the time to clinical approval. Even with the challenges precision editing faces, it’s encouraging to ...Central PMCID: PMC3922311. 6. Tabebordbar M, Zhu K, Cheng JK, Chew WL, Widrick JJ, Yan WX, Maesner C, Wu EY...