...repeating for each target. Alternatively, you can perform simultaneous detection with a mixture of primary...steps, in order to ensure your antibodies are performing their best and that the sample is not being degraded... P., & Yong, W. H. (2019). An Introduction to Performing Immunofluorescence Staining. In W. H. Yong (Ed...

...and grow along the way. That’s what makes it so wonderful. The most challenging part of jumping into this...me, the Veterans Health Administration was the perfect position because my science policy ‘why’ was to...this question may change over time and that’s perfectly normal!  Action Items:  Start a career journal...

...phenotypes. These approaches may become even more powerful following a report that one can use linear repair...non-linked loci; pha-1(e2123) mutant worms are perfectly viable at 15 ºC, yet exhibit complete embryonic...recombination repair pathway. Reagents required to perform pha-1 co-conversion are available through Addgene...

...homologous recombination has not occurred. This powerful negative selection system ensures high editing...Transcriptional repression (CRISPRi) in bacteria As RNA interference does not function in bacteria, most efforts ...inMycobacterium tuberculosisusing an efficient CRISPR interference system. Nucleic Acids Res 44:e143–e143 . https...

...“addgene” into the GIF search bar to find that perfect response GIF.     We have GIFs for every...via GIPHY   If you’re also looking for that perfect GIF for your TikTok or Instagram story, we also...

...long the scientist waited after injection before performing the experiment. Every entry has an accompanying... so that you can see for yourself how the AAV performed in that experimental setup. For example, this ...

...up a performance Ben and Niroshi organized for Tyler's (the host's) thesis defense. This wonderful piece...

...on the floor outside the lecture hall. It was wonderful to see the ad-hoc ‘sciencing’ when Dr. Prakash... By connecting art and science, a fascinating interface is created that produces loads of inspiration ...

...validity of your biological model and the technical performance of your assay. But, in many discovery-oriented...each with 4 gRNAs per kinase, so screens can be performed with either 4 or 8 gRNAs per gene. Further, the...

...time needed to go from planning an experiment to performing one.  At Addgene, over 120 labs have deposited...we currently have data for are: cut, activate, interfere, visualize, nick, purify, tag, scaffold, CRISPR-display...

...reagents) along with information critical for the performance of a reagent, e.g., antigen retrieval conditions...Knowledge-Base is an invaluable resource for anyone performing multiplexed imaging, not just IBEX. The IBEX ...

...experiments with mouse cortical neurons, far out-performing previous attempts to engineer potassium-selective...engineering to shift the spectra and improve the performance of HcKCRs. Find HcKCR plasmids here!   Govorunova...

...You’ve designed the perfect experiment – controls, conditions, and everything in between – now all you...downstream applications. Tag cleavage must be performed after primary purification and requires a second...

...therapeutic antibody that binds Activin A and interferes with inappropriate activin-ACVR1 interactions...bring HOPE to AJ.   PS: The superhero race is the perfect event for supporting AJ, who is a big superhero...

...that much tissue takes a week of multiple people performing dissections. With CUT&RUN, we have had success...up until this point. The nuclease reaction is performed on ice, and only for a short period of time, thus...

...Some use cases include: Cataloging a reagent’s performance as in this antibody ReFigure Analyzing replications...confirmatory image to show that a previously used reagent performs well, or you might publish a replication experiment...

...isolate single-cell colonies, grow them up, then perform individual genotyping (using Sanger sequencing,.... You could then confirm this purification by performing a genotyping assay (such as Sanger sequencing...

...prokaryotes originally utilized CRISPR/Cas as a powerful and adaptive defense strategy against life-threatening...The diversity of the CRISPR/Cas systems provides powerful lines of defense against invading phages. Rising...

... which studies immunity and the host-pathogen interface, previously developed a high-throughput neutralization...many different strains. It was also the first to perform neutralization studies after just the first vaccination...

...University. Stochastic multicolor labeling is a powerful solution for discriminating between neurons for... very stable in SeeDB2. However, SeeDB2 is not powerful enough for large brain samples. For large brain...