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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 291 results for: fus

Showing: 121 - 140 of 291 results

  1. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ...are artificial restriction enzymes generated by fusing a zinc finger DNA-binding domain to a DNA-cleavage...to introduce point mutations in the genome. They fused a catalytically impaired Cas9 protein to a DNA-editing...in 2019. It’s based on a prime editor which is a fusion between Cas9 and reverse transcriptase. Using an...

  2. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ...as a fusion protein. An ScFv is made up of the variable regions of the heavy and light chains fused together...produced from hybridomas. Hybridomas are formed by fusing B cells that have been extracted from animals injected...

  3. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    Published
    Feb. 27, 2024, 2:15 p.m.
    .... Empty backbones often contain epitope tags or fusion proteins that are appended to new inserts through... the gene cloned into your plasmid, any tags or fusion proteins contained in the plasmid, any mutations...affinity for calcium". Please describe any tags or fusions (e.g. His, FLAG, EGFP, etc) on your insert. Use...

  4. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ...of traditional tracing compounds is that their diffusion across the synaptic cleft is usually severely ...small (20 nm) in size which makes them capable of diffusing in the extracellular space; they also have low...there is always a risk that viral particles will diffuse long distances within the extracellular space along...

  5. A Scientist's Guide to Ebola

    Type
    Blog Post
    Published
    Oct. 16, 2014, 3:01 p.m.
    ...surface – making it critical for viral attachment and fusion. Once inside a host cell, new evidence in PLoS ...neutralizing antibodies and inhibitors of attachment and fusion. Recommended Reading For those looking to delve...

  6. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    Published
    March 21, 2019, 12:47 p.m.
    ...Plant base editing with a Cas9-adenosine deaminase fusion Caixia Gao’s lab has developed a new plant base...from an evolved tRNA adenosine deaminase that is fused to a nicking Cas9. This base editor can be used ...

  7. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    Published
    June 17, 2014, 7:57 p.m.
    ... is fused to a translational repressor. In principle, however, designer PUF proteins can be fused to other...

  8. What's New in CRISPR - December 2019

    Type
    Blog Post
    Published
    Dec. 17, 2019, 2:37 p.m.
    ... and A-to-G base editors into one protein. They fused an activation-induced cytidine deaminase (AID) and...adenosine-to-inosine (A-to-I) RNA editor called REPAIR by fusing catalytically inactivated RNA-targeting Cas13 (...

  9. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    Published
    Nov. 19, 2013, 2:37 p.m.
    ... e67902. Many imaging studies rely on direct fusion of fluorescent proteins (FPs) to a yeast gene of...epitope tag for creation of your own unique protein fusions. This collection is ideal for tandem affinity purification...

  11. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    Published
    June 30, 2015, 2 p.m.
    ...The first functional SB system was synthesized by fusing and modifying various extinct transposases. Improvements...is made up of a fragment of the RPL13a promoter fused to a region of the RPL41 gene, to drive the selection...

  12. A Tour of Addgene's Most Popular Pooled Libraries

    Type
    Blog Post
    Published
    Nov. 19, 2025, 2:15 p.m.
    ...these applications, however, nuclease-dead Cas9 is fused to a transcriptional activator or repressor to create...libraries In surface display, proteins of interest are fused to anchoring proteins to be expressed on the surface...

  13. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ... common protein-only transduction strategy is to fuse a cell penetrating peptide (CPP) to a protein of...discovered in the HIV protein Tat. Although CPP fusions can translocate a cell membrane, the presence of...

  15. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    Published
    June 18, 2019, 1:15 p.m.
    ...useful for studies in non-human primates, where fusion fluorescent proteins can affect DREADD trafficking... (PV2527) 105679  AAV5 ppAAV-CKIIa-stGtACR1-FusionRed New tetracycline transactivators Plasmid...

  16. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    Published
    April 18, 2019, 12:53 p.m.
    ...in human cells (Ma et al., 2015). To do so, they fused a different fluorescent protein to various Cas9 ...Cas9 variants, SpCas9, NmCas9, and St1Cas9. These fusions localized to specific loci based on the guide RNA...

  17. DAP arrays for multiplex gene editing

    Type
    Blog Post
    Published
    July 7, 2022, 1:15 p.m.
    ...lab first thought to enable MBE by using Cas12a fused base editors (dCas12a-BE). However, the poor editing...efficiencies they found led them back towards Cas9 fused base editors (nCas9-BE), as Cas9 is mechanistically...

  18. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    Published
    Sept. 8, 2015, 2:30 p.m.
    ...with other pathways. Since these effectors are diffusible, they’re also not suitable for spatially limited...light-sensitive two-component system (TCS). Levskaya et al. fused a photosensory domain from cyanobacteria to a common...

  19. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    Published
    March 25, 2014, 3:15 p.m.
    ...not appropriate for mammalian selection – do not confuse this with G418 (aka Geneticin). Neomycin should...mammalian expression – instead use G418. This can be confusing since the neo/kan gene confers G418 resistance...

  20. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    Published
    July 22, 2025, 1:15 p.m.
    ...luminescent reaction when they come together. By fusing each luciferase subunit to other proteins, researchers...A luciferase and a fluorescent protein are each fused to one of the proteins being tested for interaction...
Showing: 121 - 140 of 291 results