We narrowed to 513 results for: gats

....e. restriction cloning, Gibson Assembly, Golden Gate etc.), and try to find the simplest way to do it...Transformation Transform a few microliters of your ligation reaction. Here are the possible outcomes: No ...PCR, putting special attention in the areas of ligation between the different fragments If you don’t ...t get any positive clone, try transforming the ligation mix in different bacterial strains (stbl2, NEB...

...Library, deposited by Carrie Bhang, a research investigator in the In Vivo Pharmacology group at Novartis...the parent cell population using the library, propagated it, separated it into roughly identical replicate...ClonTracer Since starting her new role as an investigator in the In Vivo Pharmacology group at Novartis...Addgene community! Carrie Bhang is a research investigator in In Vivo Pharmacology at Novartis Oncology...

...timeline of the Golden Gate (GG) cloning approach. Individual modules and a sample ligation are depicted. (Bottom...assemble them into a destination vector via Golden Gate cloning, perform an exonuclease cleanup, transform...Cloning Addgene's CRISPR Guide Plasmids 101: Golden Gate Cloning...

...follow through on their promises, Wet-blanket negativist, who can bring down the morale of a group with...Some supervisors might try to intimidate you with negative comments (hostile-aggressives, complainers, know-it...know-it all experts, wet-blanket negativists, and micromanagers), while other supervisors might be nice...complete your work. Whether your supervisor is a negative person or just an inexperienced manager, you can...

...detect mEPSPs, it also enabled imaging of their propagation along dendrites for the first time (Figure 3)...without the Kv motif. Altogether, if you want a negative-going GEVI that can be used in different illumination...include large-scale pharmacological profiling or interrogation of context-dependent signaling in primary cells..., J. C., & Garcia-Marcos, M. (2024). Direct interrogation of context-dependent GPCR activity with a universal...

...interest. Select for cells of interest (positive or negative selection). Next-generation sequencing (NGS) to...high degree. This is particularly helpful when investigating tissue-specific diseases, as you can target ...protein altogether. Platforming AAV Interest in interrogating and mapping the genetic landscape of tissue-...used to map tumor suppressors in mouse liver, interrogate immune cells to improve immunotherapy in glioblastoma...

...days Purification Ultracentrifugation (AAV); centrifugation (lenti) Centrifugation Titration time ~...is typically purified using methods like ultracentrifugation, while lentivirus supernatants are centrifuged...

... iodixanol gradient ultrafugation Overview: Iodixanol gradient ultracentrifugation uses a gradient of ...during AAV production is overlaid. Following ultracentrifugation, the AAV-containing 40% fraction is collected...day. Refer to the AAV Iodixanol Gradient Ultracentrifugation Protocol for more details. Pro tips Watch...

...collection is a set of vectors for use with the Gateway Cloning Platform (Life Technologies, Carlsbad, ...involved in the Ras pathway alone and thousands of investigators studying them, how do we ensure that the research...Cancer Research Plasmids and Resources page, investigators may select a pathway and identify any genes ... click on a gene of interest will direct the investigator to a list of all available Addgene plasmids ...

...-wide screens to effectively and efficiently investigate gene function. CRISPR libraries can be used to...To test the library, they conducted genome-wide negative selection screens with both A375 (melanoma) and...similarly to the Brunello knock-out library while mitigating toxicity induced by high copy number dsDNA cutting...libraries. These unique hits may have been false negatives in the other screen, implying that ORF and CRISPRa...

...to spend their days microinjecting fluorescent conjugates into cells. Fortunately, over the last 20 years...Stains – These labels consist of a fluorophores conjugated to molecular species with high affinity for endogenous...fluorophore attachment, the brightness of the resulting conjugate, and the complexity of the system. A particularly...refinements to these systems—smaller tags, brighter conjugates, higher fluorogenicity, photoactivation etc.—...

...machinery within the cell. Most plasmids encode a negative regulation system involving antisense RNAs or ...).  In cases of compatible plasmids, different negative regulation systems control plasmid replication...plasmids have the same origin of replication, the negative regulation system cannot distinguish between the... the different plasmids. The concentration of negative regulation system elements in the cell is artificially...

...in a range of biological studies, including investigations into environmental pollutants and health, embryo...developmental biology that arose from zebrafish-based investigations. Genetic resources for zebrafish A common ...about the genome are powerful resources for investigating any biological process. High quality genome ...are particularly well-suited for neuroscience investigations. For example, neuronal activity can be tracked...

...months, in fact. In the Lee-Parsons lab, we are investigating how the production of cancer therapeutic drugs...expression method enables us to quickly screen and investigate transcription factor candidates and the regulation...transient methods are usually the first choice for investigating gene function, as these experiments can be done...different ideas. We are a huge fan of the Golden Gate based MoClo system. The vectors mentioned in this...

...pieces of DNA whose sole purpose is to ensure the propagation of themselves and their associated genes. These...specific 700 bp segment of DNA, the plasmid was propagated better than plasmids without this DNA. They eventually...Perhaps the most widely used application of CcdB is GatewayTM cloning, where the ccdB gene serves as a counterselectable...Thomas, and Kenn Gerdes. "Mechanism of post-segregational killing by the hok/sok system of plasmid R1:...

...norepinephrine to promote vigilance. A recent study investigated the injection of AAV8 particles expressing an....   A study from 2014 (Howerton at al. 2014) investigated the connection between neuroinflammation and... expression of miR124a, a regulatory RNA that negatively regulates BDNF levels. They found that downregulating...prefrontal cortex (mPFC). In 2019, the Duman lab investigated this further and showed that the activity of...

...The 3’ end of the non-invasive strand is elongated and ligated to fill the gap, thus completing SDSA.   ...-independent pathway as it involves the direct ligation of sticky or blunt ends and often introduces small...four-stranded branched structures that form when elongation of the invasive strand “captures” and synthesizes...

...for cloning into the final translated products. Negative selection markers built into all plasmids in the...Gagoski used SITS to create the pCell-Free vectors, Gateway-compatible backbones that enable cell-free expression...for product integrity, expression level, and aggregation propensity in four expression systems: E. coli...base RNA sequence. The segments are provided in Gateway compatible vectors that allow you to quickly clone...

...adenosine of the GATC recognition site. Transformation: After the PCR reaction, no ligation is required since...from these overhangs and ligate these blunt ends together in a standard ligation reaction resulting in a...

...success. Gather data on the lab choices How do you find out what the lab is really like? Gather data of... lab website, publication records, LinkedIn, ResearchGate or Twitter. Be ready for the interview with ...Alumni meetings can be the most informative way of gathering data. Former lab members can give open, revealing...