...nucleases, recombinases) which irreversibly excise or invert a specific chunk of DNA. However, a limitation ...genome-engineering. One particularly attractive application involves using dCas9-activator fusion proteins to induce...a “neuron-like” phenotype when targeting genes involved in neurogenesis, which had proven challenging ...identifying previously unknown factors and pathways involved in pre-mRNA splicing in a compound screen. ...visualize the fusion in vitro or in vivo. The protocol involves isolating total RNA, resolving by PAGE and then...

...yet powerful way to tag cellular proteins and investigate protein localization, interaction, and expression... of the most important applications for PA-FPs involve enhancing optical live imaging of proteins, organelles...and cells. Similar to FPs, PA-FPs allow for non-invasive labelling, but in contrast, PA-FPs offer a reduced...

...Stability of fluorescence: For applications that involve ranges of pH, and significant lengths of time, ...incorporation of fluorophores, as seen in studies involving dopamine D2/D3 receptors (Allikalt et al., 2020...processes like mitochondrial dynamics. This process, involving mitochondrial homeostasis maintained through mechanisms...

...been identified in humans and cataloged in the ClinVar database. Previously developed genome editing methods...prime editing efficiency can vary widely — so its inventors kept optimizing it. Using a pooled CRISPRi screen...Programmable deletion, replacement, integration and inversion of large DNA sequences with twin prime editing...

... Singapore. Chapter 4: Life as a new primary investigator. Coming up with new project ideas, applying ... Chew! Wei Leong Chew is a Junior Principal Investigator at the Genome Institute of Singapore. His lab...

...lab manager, research technician, or Principal Investigator. How-to guides for troubleshooting your experiments...believe that science gets better when everyone gets involved. So join the conversation! Do you have a question...

...scientific progress made through years of public investment isn't lost. Universities and research institutions...benefit of everyone. We Want to Hear from You We invite researchers to share how these funding changes ...

...exciting way of listening to microbes by using non-invasive methods for cellular imaging with ultrasound. ...Raymond W., et al. "Acoustic reporter genes for noninvasive imaging of microorganisms in mammalian hosts....

...ring that glows with a faint green light. To investigate this green glimmer, Shimomura collected many,... in learning more about fluorescent proteins, I invite you to download our Fluorescent Protein eBook and...

...Rights to all research results, modifications, and inventions created by the Recipient Right to file patent...patent applications on modifications or inventions Right to publish without editorial comment or review...

...there’s the opportunity cost of your time; an investment of months of your life that may end with little...assay or both, and you have no idea what genes are involved. Or you don’t know how well your assay will scale...

...sabatini-wood-cancer-pathway-orfs/ Finding signaling pathways involved in drug resistance Wood wanted to find out which...environments, for example. “We’ve been contacted by investigators all over the world who are interested to use...

...information back. No team has all the answers – reinventing the wheel is inefficient. An effective team regularly...worry that it seems like "tattling" if others are involved. They may feel they are wasting their time. The...

...University. There are tons of ways you can get involved in science communication. In this second half ...science communication student group and get more involved with your local community. What do Vini and Amy...

...Annotation of genes in immune cells typically involves the creation of germline knockout mice, which ...To demonstrate the power of these systems to investigate in vivo mechanisms, we targeted two phosphatases...

...current postdocs will be academic principal investigators. She also pointed out promising new practices...Your PI”.   She focused her talk on the issues involved with the fact that the PhD and Postdoc advisors...

...plasmids to study the dimerization of HLA B27, involved in the onset of spondylarthritis, using Bioluminescence...than discussing a new scientific discovery or invention, and sharing those ideas with others. This love...

...melanoma model, they also went in search of genes involved in resistance to the cancer drug vemurafenib. ...lab has used their library to screen for genes involved in bacterial toxin resistance, identifying 4 previously...

... famous role as the “happy hormone,” it’s also involved in reinforcement learning, decision making, and...dopamine and other biosensors in our AAV vector inventory. References Darvesh, Altaf S., et al. "In vivo...

...arg1 vesicles. Following collapse, vesicles are invisible to ultrasound, which allows for differentiation...Shapiro, M.G. (2018). Acoustic reporter genes for noninvasive imaging of microorganisms in mammalian hosts....