...making wide-scale gene activation seem next to impossible. The development of CRISPR/Cas systems, however...rely on fusion proteins while others re-engineer components of Cas systems themselves. SunTag, SAM, and VPR... fusion protein, rather than relying on a two-component system dependent on gRNA design (SAM) or peptide...lines (Sajwan et al., 2019). SPH SPH combines components of SAM and SunTag activators, fusing the epitope...

... Figure 1: SARS-CoV-2 proteins and other components. Image from Maya Kostman for the IGI.     ...Addgene to better understand which human genes are important for SARS-CoV-2 entry into human lung epithelial...). In their search, they found mucins have an important role in viral entry, which are inhibitors of SARS-CoV...research through sharing reagents has never been so important.     References and resources References ...

...conclusions. Being vigilant about these errors is an important part of maintaining the quality of reagents, especially... especially shared ones.  The importance of QC Plasmids are a fundamental part of research. They are ...tags, promoters, and other critical functional components. Fun fact! Some older plasmids in our collection...each of their plasmids, from descriptions of key components of the plasmids to partial, full, or annotated...

...transcribed but do not encode proteins. They play important roles in many cellular and regulatory processes...into the break sites. Knock-in experiments are important for visualizing ncRNA localization and function...complex and not yet fully understood, so it is important to carefully select the target locations of knock-in...nuclease-dead Cas9 protein (dCas9) and a sgRNA to temporarily block the transcription machinery of a target...

...One such control is dividing up the essential components of a virus into different plasmids. For a retroviral... are not encoded in the transfer plasmid, the component that physically integrates into the host genome... Figure 1: Separation of retroviral packaging components onto distinct plasmids for safety.    Viral... would be infected by a lab-grown virus, it's important to understand the risks of the specific virus ...

...assembly. Simply put, it assembles individual DNA components into larger units or circuits with functional...Transcription units (TUs), which are functional units composed of multiple parts transcribed together. They can... test a variety of different orientations and components within the system to maximize production potential...multiplexed gRNA systems as well as additional components such as selectable markers, promoters, enhancers...

... sections as a primary research article, it’s important to understand that presenting your work in poster...certain posters resonate with you (or not). It is important to reflect on these impressions when thinking ...concise as possible, communicating only the most important results to your audience. With a poster, you have...particular styles recognized to work well. It is important that your readers can navigate your poster and...

...to make sure that everyone can be informed and empowered to make their own decisions about genetics, both...their academic environment and attend seminars, symposia, or courses in social, health or policy-related...either giving or running these events will be important both in preparing you for future work in these...communicate your work to a non-technical audience is an important skill for every scientist. After all, scientific...

...better at producing a compound of interest, thus allowing them to optimize the compound production process...Although it is possible to make a timer system composed of two separate FPs (one that matures quickly ...Chronology of islet differentiation revealed by temporal cell labeling." Diabetes 58.8 (2009): 1863-1868...

... strategy uses new combinations of PE protein components (Figure 1), an altered epegRNA delivered with...protein-coupled receptors (GPCRs) are critical components of many physiological pathways and represent ...et al., 2024). Each plasmid expresses all the components of the biosensor in optimal proportions from ...Waldron We recently added two antibodies against important targets for neurodegeneration research: Anti-MAPT...

...individual libraries can vary significantly, it’s important that we have an amplification protocol developed...and the desired coverage for the library (How important is it that every single plasmid in your library...protocol. Created with BioRender.com.   The most important factor to consider is your desired library coverage...used for libraries that are similar in size and composition as examples. Table 1 provides an overview of ...

...detector using a series of mirrors and filters. Importantly, conventional flow cytometry uses one detector... flow cytometry for a given experiment, it is important to consider how many parameters you want to use...conventional and spectral flow cytometry, it is important to consider the expression levels of the markers...two highly overlapping cellular markers, it is important to pair them with two fluorophores that have distinct...

...other systems reviewed here. The only required components are 1) a SMASh-tagged protein and 2) asunaprevir...reviewed here. The AID system relies on three components. First, the protein of interest must be AID-tagged... the system is that it requires an additional component to be engineered or expressed beyond the tag and...SMASh). If you don’t want to deal with additional components beyond the minimum required for a degron system...

... wild populations. Whereas most genomic changes impose a fitness cost and are eliminated by natural selection... wild ones. For example, Drosophila lines with compound autosomes are completely infertile when mated ...with appropriate measures. Barriers should be a component of all gene drive confinement strategies, but ...: I. Cage Studies of Chromosome Replacement by Compound Autosomes in Drosophila melanogaster. Genetics...

...chose to re-examine our global data at a finer temporal granularity. For each paper, we observed that ...the likelihood in our Poisson process into two components: 𝜃x(t) and 𝜓z(t). 𝜃x(t) describes the exponential...generally not be the same if weekends were somehow imposed on scientists. Figure 6: Model fit. Orange...parameters from relatively sparse data from China. An important additional caveat is that our dataset contains...

...Genetically modified organisms like transgenic mice are important tools for understanding the function of cell types...CRISPR edits and improves editing efficiency. Importantly, the point mutations created by Agudelo et al...length with TZAP Maintaining telomere length is important for cellular function and genomic stability, as...multiplex gRNA expression. Expression of toolkit components can be controlled by a variety of promoters for... for versatile and robust engineering. These components are provided in Golden Gate compatible backbones...

...External collaborations are becoming increasingly important as more complex approaches to scientific questions...collaborations and partnerships are also very important in a non-research context, especially when considering...start and maintain external collaborations is important? In my current job at Addgene I work with people...work with others outside your organization is important for success in research-intensive careers. In ...

...FLP-FRT. In addition, a variety of means to spatiotemporally control FLP and Cre expression have been developed...These differences in recombinase efficiency are important to keep in mind when deciding whether to use the... only occur at a specific time point? To allow temporal control, ligand regulated Cre and FLPe recombinase...instance) can be controlled with FLP-FRT. For further temporal control, Tamoxifen inducible Cre versions can ...

...great for studying parenting strategies and make important ecological indicators since species live in water...Aiptasia - algae Breviolum minutum symbionts are important models for solutions to the coral reef crisis....chemical pathways generating interesting drug-like compounds especially cardenolides which have been used to... interesting biologically. They have economic importance as harmful weeds. The parasitic fusion is such...

...making the materials available to many countries, import and export regulations, differing customs processes...In China, direct shipments weren’t able to clear import control. Partnering with a distributor allowed ...Addgene YouTube channel to add a strong visual component to our educational resources. From 2013–2023, ...expanding our website, we’ve learned about the importance of smaller, systemic changes, like using alt-...