We narrowed to 136 results for: tox
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TypeBlog PostPublishedJune 17, 2025, 1:15 p.m....specificity, so in some cases they can lead to toxicity and off target effects. More recently, shRNA plasmids...
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Viral Vectors 101: Integrase-Deficient Lentiviral Vectors
TypeBlog PostPublishedMay 13, 2025, 1:15 p.m....Cas9 is no longer necessary. However, Cas9 can be toxic if continuously expressed (Fu et al., 2013). Using... -
Is this the right place for me? 8 tactics for choosing a lab
TypeBlog PostPublishedOct. 2, 2018, 12:56 p.m.... choose wisely. From the Tenure She Wrote blog, Toxic Academic Mentors. What is workplace bullying? ... -
Important Considerations When Using AAVs
TypeBlog PostPublishedJune 13, 2017, 2:30 p.m....tracing methods used viruses that were potentially toxic and would result in cell death. With several new... -
Quick Guide to All Things Lentivirus
TypeBlog PostPublishedMarch 21, 2017, 2:30 p.m....constitutively or inducibly. If your gene of interest is toxic or needs to be expressed a certain point in the ... -
An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency
TypeBlog PostPublishedDec. 17, 2020, 1:30 p.m....repair templates. They found that Cas9 RNPs could be toxic at high concentrations, and editing efficiency improved... -
RNA Interference in Plant Biology: New Tools for an Old Favorite
TypeBlog PostPublishedOct. 27, 2020, 1:15 p.m....organisms with functional APT will convert 2-FA to cytotoxic nucleotides, resulting in death. In the presence... -
Mycoplasma Contamination: Where Does It Come From and How to Prevent It
TypeBlog PostPublishedOct. 8, 2020, 1:15 p.m....removal from the infected culture. MRAs are non-toxic, effective in the removal of most of the mycoplasma... -
Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering
TypeBlog PostPublishedDec. 15, 2016, 3:57 p.m.... of Red proteins is critical for minimizing the toxic effects of Gam expression and to limit spontaneous... -
Sequencing Options for CRISPR Genotyping
TypeBlog PostPublishedOct. 4, 2016, 2:30 p.m....multiplexing Requires delivery of dsODN (potentially toxic) Digenome Seq 0.1% Cell-free (in vitro) Works ... -
Custom CRISPR Screens & the Green Listed Software
TypeBlog PostPublishedJuly 11, 2017, 2:30 p.m....phenotype (e.g. survival of a cancer cell as a cytotoxic drug is added), and decide which genes you’d like... -
Special Delivery: Fluorophore Targeting for FRET Studies
TypeBlog PostPublishedJuly 19, 2016, 2:30 p.m....was originally developed to treat heavy metal intoxication in chemical warfare [Vilensky & Redman, 2003... -
Antibody Validation for Flow Cytometry
TypeBlog PostPublishedSept. 10, 2024, 1:15 p.m....target with very low expression, due to induced toxicity caused by the protein of interest. HLDA workshop... -
CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More
TypeBlog PostPublishedSept. 28, 2020, noon...system has generally low efficiencies or can be proteotoxic when used in Mycobacterial species, Sarah Fortune... -
Troubleshooting and Optimizing a Western Blot
TypeBlog PostPublishedSept. 17, 2024, 1:15 p.m....Western Blotting. Journal of Pharmacological and Toxicological Methods, 64, 233–237. https://doi.org/10.1016... -
Viral Vectors 101: Systemic Capsids
TypeBlog PostPublishedOct. 12, 2023, 1:15 p.m....susceptible to off-target transgene expression-induced toxicity in NHPs (Hordeaux et al, 2020; Hudry et al, 2023...