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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 260 results for: uros

Showing: 121 - 140 of 260 results

  1. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    Published
    June 24, 2020, 5:45 p.m.
    ...-dCas9-p300 Core, as well as pLV-dCas9-p300-P2A-PuroR for lentiviral expression. Figure 1: dCas9... deposited pdCas9-DNMT3A-EGFP and pdCas9-DNMT3A-PuroR for targeted cytosine methylation in mammalian cells... cells. Co-expression markers EGFP and PuroR enable sorting and selection of transduced cells. Grant Challen...

  2. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    Published
    March 13, 2014, 4:08 p.m.
    ...marker such as puromycin, you can (also) select for the positive colonies by puromycin selection. You ...transfected cells (for example, plasmid with a puromycin resistance cassette, such as PX459). After you...

  3. Hot Plasmids Podcast Episode 2: New RFPs, AAVs, & More

    Type
    Blog Post
    Published
    Sept. 8, 2017, 7:52 p.m.
    ...generating zebrafish transgenic lines to study neurodegenerative diseases. Listen to Our Segement on the Tol...Plasmid 2: AAV Vectors for tageting GABA-ergic interneurons. Listen to Our Segment on these new AAV Vectors...

  4. Addgene at SfN23!

    Type
    Blog Post
    Published
    Nov. 9, 2023, 2:15 p.m.
    ...Getting ready for Society for Neuroscience Conference 2023? So are we! Come say hi to us at Addgene's...Conference New to SfN? Try A Novice's Perspective on Neuroscience - SfN17!   ...

  5. Newly Updated AAV Data Hub!

    Type
    Blog Post
    Published
    Feb. 7, 2023, 2:15 p.m.
    ... connections between BLA projection neurons and mPFC interneurons can be labelled with an AAV1 Cre virus...

  7. Cre-ating New Methods for Site-specific Recombination in Drosophila

    Type
    Blog Post
    Published
    May 12, 2015, 1:32 p.m.
    ...test recombination efficiency. When expressed in neuronal cells, each line showed broad recombinase-dependent...hamster ovary (CHO) cells, as well as in cortical neurons in layer 2/3 of the mouse brain. Rubin’s work shows...

  9. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    Published
    Sept. 21, 2021, 1:15 p.m.
    ...CD90.1 selection marker, Thy1.1, in addition to puromycin resistance.  The MYC-CRISPR library targets E-...AAV tools to selectively control parvalbumin interneurons from the Dimidschstein Lab More GCaMP8 ready-to-use...

  10. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    Published
    Nov. 18, 2016, 3 p.m.
    ...and chemogenetics communities to activate neurons and neuronal pathways using light and chemicals respectively...

  12. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    Published
    March 1, 2018, 2:11 p.m.
    ...for successful transduction. In another example, neuronal gene expression by an anterograde AAV was shown...input-defined neural pathways for defense behaviors." Neuron 93.1 (2017): 33-47. PubMed PMID: 27989459. PubMed...

  13. Celebrate National Book Month with Addgene

    Type
    Blog Post
    Published
    Oct. 3, 2019, 12:47 p.m.
    ...Tales of Music and the Brain by Oliver Sacks "Neurologist Oliver Sacks examines the relationship between...Musicophilia includes case studies of relevant neurological atypicalities, including a patient with amusia...

  15. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    Published
    May 21, 2019, 12:10 p.m.
    ... treated HeLa cells expressing FlipGFP with staurosporine, which induces apoptosis by activating caspase...array of diseases and pathologies such as neurodegenerative diseases, autoimmune disorders, and cancer...

  16. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    Published
    March 14, 2019, 1:54 p.m.
    .... Exo-AAV9 was successfully shown to transduce neurons in the brain after systemic (i.v.) delivery in ...stromal cells after intravenous delivery." Journal of neuro-oncology 139.2 (2018): 293-305. PubMed PMID: 29767307...

  17. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    Published
    May 26, 2020, 1:15 p.m.
    ...stably integrate a reporter gene that contains a puromycin resistance gene followed by the target site (the...genome edit:  A donor plasmid that contains a puromycin resistance gene linked to an in-frame H2B-GFP....

  18. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    Published
    April 5, 2022, 1:20 p.m.
    ... recombinant antibody resource called NABOR (Neuroscience AntiBody Open Resource). The antibodies distributed...of monoclonal antibodies publicly available. The Neuro Mab Sequencing Initiative uses high-throughput DNA...

  20. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    Published
    July 21, 2016, 2:30 p.m.
    ...international, collaborative project—funded by the European Commission, the US National Science Foundation...increasingly, corporations in the US and Western Europe have taken three strategic approaches to gender...
Showing: 121 - 140 of 260 results