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We narrowed to 842 results for: NTS

Showing: 701 - 710 of 842 results

  1. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    Published
    March 20, 2014, 3:04 p.m.
    ...and me. To that end, here are my personal 10 commandments for making it all work – most of the time. #1...transportation, call a cab and remember their own school assignments, the better. In my opinion, a level of independence...

  2. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    Published
    July 13, 2023, 1:15 p.m.
    ...Usage of a pooled library lentiviral prep for experiments. Pooled library lentiviral preps are generally...for sequencing, representative NGS results (read counts or another visualization) that confirm diversity...

  3. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    Published
    Sept. 16, 2020, 1 p.m.
    ...it leaves little room for additional regulatory elements. Feng Zhang's group previously packaged Cas9 and...Resources on the Addgene Blog Learn more about Cas9 Variants and the Expanded CRISPR Toolbox Read about CRISPR...

  4. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    Published
    July 25, 2024, 1:15 p.m.
    ...molecules that may bind non-specifically to assay components and increase background staining. Direct ELISAs...with binding, and some common antibody buffer components can interfere with conjugates. Make sure to do...

  5. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    ...mechanism which exclusively yields non-crossover events. This means all newly synthesized sequences are...PLoS ONE 9(8); (2014). PubMed. Making designer mutants in model organisms. Peng, Y. et al. Development...

  6. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ...exposure. However, the limited availability of reagents and equipment and the long turnaround times, have...steps and the Cas protein used. Here, all the components needed for the reaction are mixed in a single...

  8. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ...transfections and are handy for quick in vitro experiments. shRNA plasmids typically use RNA polymerase ...needs to be taken into account when designing experiments.   {% module_block module "widget_0c4dbd65-f69b...

  10. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    Published
    Nov. 16, 2021, 2:15 p.m.
    ...isotype controls are also commonly used in IF experiments. Precisely which controls you perform will depend...spectral properties match your available imaging instruments and the spectral properties do not overlap with...
Showing: 701 - 710 of 842 results