We narrowed to 842 results for: NTS
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TypeBlog PostPublishedJan. 10, 2023, 2:15 p.m....digest appears normal and produces the expected fragments for that plasmid. This phenomenon indicates the...strategies for resolving multimers? Let us know in the comments below or contact us at [email protected]. Resources...
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CRISPR 101: Epigenetics and Editing the Epigenome
TypeBlog PostPublishedJune 24, 2020, 5:45 p.m....about your experience with these constructs in the comments! Find Plasmids for Epigenetic Modification ...high-throughput screening for functional regulatory elements in the human genome. Nature Biotechnology 35:561... -
Viral Vectors 101: AAV Serotypes and Tissue Tropism
TypeBlog PostPublishedJan. 16, 2025, 3:42 p.m.... cell types. Figure 1: Diagram of AAV components. Created with BioRender.com. AAV vectors .... Directed evolution of a family of AAV capsid variants enabling potent muscle-directed gene delivery ... -
Antibodies 101: The Basics of Western Blotting
TypeBlog PostPublishedMarch 8, 2021, 2:41 p.m....gauge protein abundance they provide qualitative measurements. If you require precise quantification of a ...that isolates proteins from different cellular compartments such as the mitochondria, cytoplasm and nucleus... -
PiggyBac-ing Through the Genome Editing Field
TypeBlog PostPublishedMay 31, 2016, 3:30 p.m....1]. Like other transposons, piggyBac™ has two components, a transposon and a transposase. The piggyBac...piggyBac™ transposase or hyperactive transposase mutants [7], it is generally not as efficient as Cre recombinase... -
Quick Guide to All Things Lentivirus
TypeBlog PostPublishedMarch 21, 2017, 2:30 p.m....fantastic options for delivering shRNA, CRISPR/Cas9 components, and fluorescent sensors. Retroviral vector ...you will find this information useful for your experiments. Don’t hesitate to check our lentiviral vector... -
A Needle in a Base-Stack: Cas9 Structural Biology
TypeBlog PostPublishedJune 4, 2024, 1:15 p.m....CRISPR 101 eBook Resources on the Addgene blog Components of CRISPR xCas9: Engineering a CRISPR Variant...2018). Real‐time observation of flexible domain movements in CRISPR–Cas9. The EMBO Journal, 37(10), e96941... -
How to Deposit Your Plasmids with Addgene
TypeBlog PostPublishedFeb. 27, 2024, 2:15 p.m....more genes, protein coding sequences, or other elements inserted into it. You will have the option to ...plasmid is designed to allow you to produce large amounts of your insert in E. coli, then this is a Bacterial... -
Viral Vectors 101 eBook 2nd Edition
TypeBlog PostPublishedJuly 11, 2024, 1:15 p.m....find an introduction to viral vectors and their elements; resources on different types of vectors, capsids... -
CRISPR Guide Update
TypeBlog PostPublishedOct. 10, 2024, 1:15 p.m....describe newly engineered Cas9s with more flexible requirements and enhanced editing, as well as alternatives...