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We narrowed to 855 results for: NTS

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Showing: 721 - 730 of 855 results

  2. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ...transfections and are handy for quick in vitro experiments. shRNA plasmids typically use RNA polymerase ...needs to be taken into account when designing experiments.   {% module_block module "widget_0c4dbd65-f69b...

  3. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    ...mechanism which exclusively yields non-crossover events. This means all newly synthesized sequences are...PLoS ONE 9(8); (2014). PubMed. Making designer mutants in model organisms. Peng, Y. et al. Development...

  4. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    Published
    March 8, 2021, 2:41 p.m.
    ...gauge protein abundance they provide qualitative measurements. If you require precise quantification of a ...that isolates proteins from different cellular compartments such as the mitochondria, cytoplasm and nucleus...

  5. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    Published
    June 24, 2020, 5:45 p.m.
    ...about your experience with these constructs in the comments! Find Plasmids for Epigenetic Modification   ...high-throughput screening for functional regulatory elements in the human genome. Nature Biotechnology 35:561...

  6. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ...digest appears normal and produces the expected fragments for that plasmid. This phenomenon indicates the...strategies for resolving multimers? Let us know in the comments below or contact us at [email protected]. Resources...

  7. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    Published
    May 31, 2016, 3:30 p.m.
    ...1]. Like other transposons, piggyBac™ has two components, a transposon and a transposase. The piggyBac...piggyBac™ transposase or hyperactive transposase mutants [7], it is generally not as efficient as Cre recombinase...

  8. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    Published
    March 21, 2017, 2:30 p.m.
    ...fantastic options for delivering shRNA, CRISPR/Cas9 components, and fluorescent sensors. Retroviral vector ...you will find this information useful for your experiments. Don’t hesitate to check our lentiviral vector...

  9. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    Published
    June 4, 2024, 1:15 p.m.
    ...CRISPR 101 eBook  Resources on the Addgene blog Components of CRISPR  xCas9: Engineering a CRISPR Variant...2018). Real‐time observation of flexible domain movements in CRISPR–Cas9. The EMBO Journal, 37(10), e96941...

  10. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    Published
    Feb. 27, 2024, 2:15 p.m.
    ...more genes, protein coding sequences, or other elements inserted into it. You will have the option to ...plasmid is designed to allow you to produce large amounts of your insert in E. coli, then this is a Bacterial...
Showing: 721 - 730 of 855 results