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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 242 results for: HTT

Showing: 71 - 80 of 242 results

  1. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type
    Blog Post
    Published
    July 7, 2020, 1:15 p.m.
    ... DNA without DNA cleavage. Nature 551:464–471 . https://doi.org/10.1038/nature24644 Glaser A, McColl B...Editing. Molecular Therapy - Nucleic Acids 5:e334 . https://doi.org/10.1038/mtna.2016.48 Katti A, Foronda ...activity. Nucleic Acids Research 48:2841–2852 . https://doi.org/10.1093/nar/gkaa124 Martin AS, Salamango...APOBEC-Cas9 editosome complexes. Scientific Reports 9: . https://doi.org/10.1038/s41598-018-36739-9 St. Martin ...living cells. Nucleic Acids Research 46:e84–e84 . https://doi.org/10.1093/nar/gky332 Standage-Beier K, Tekel...human cells. Nucleic Acids Research 47:e120–e120 . https://doi.org/10.1093/nar/gkz713 Additional resources...

  2. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ...nucleobase detection and identification using FnCas9. https://doi.org/10.1101/2020.04.07.028167 Broughton JP... detection of SARS-CoV-2. Nature Biotechnology. https://doi.org/10.1038/s41587-020-0513-4 Kellner MJ, ...CRISPR nucleases. Nature Protocols 14:2986–3012 . https://doi.org/10.1038/s41596-019-0210-2 Lucia, C., Federico...on CRISPR-Cas12. Cold Spring Harbor Laboratory. https://doi.org/10.1101/2020.02.29.971127 Rauch, J. N....Genetic Material. Cold Spring Harbor Laboratory. https://doi.org/10.1101/2020.04.20.052159 Zhang, F., Abudayyeh...detection of COVID-19 using CRISPR diagnostics. https://www.broadinstitute.org/files/publications/special...

  3. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    Published
    May 23, 2023, 1:15 p.m.
    ...multiplex immunolabeling of brain. eLife, 8, e43322. https://doi.org/10.7554/eLife.43322 Brewer, M., McDonough...Immunofluorescence on Fresh Frozen Tissue-V2 v2. protocols.io. https://doi.org/10.17504/protocols.io.bs68nhhw Im, K.,...Biobanking (Vol. 1897, pp. 299–311). Springer New York. https://doi.org/10.1007/978-1-4939-8935-5_26 Jambor, H...scientific publications. PLOS Biology, 19(3), e3001161. https://doi.org/10.1371/journal.pbio.3001161 McLaughlin...Immunohistochemistry. Materials and Methods, 9. https://doi.org/10.13070/mm.en.9.2846 Radtke, A. J., Kandov...National Academy of Sciences, 117(52), 33455–33465. https://doi.org/10.1073/pnas.2018488117 Additional Resources...

  4. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...–1547.e15. doi: https://doi.org/10.1016/j.neuron.2025.05.002. bioRxiv preprint: https://doi.org/10.1101...deactivation rates. bioRxiv, 2025.03.20.643984. https://doi.org/10.1101/2025.03.20.643984.  Build your...retrotransposon activity to new DNA sites. Nature. doi: https://doi.org/10.1038/s41586-025-08877-4.  Two powerhouse...interactome. Molecular Cell, 84(24), 4929-4944.e8. https://doi.org/10.1016/j.molcel.2024.11.007. Branon, ... TurboID. Nature Biotechnology, 36(9), 880–887. https://doi.org/10.1038/nbt.4201. Hot viral preps: Enhancer-AAVs...

  5. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    Published
    March 8, 2021, 2:41 p.m.
    ...protein analyses using ELISA. Peptides 72:4–15 . https://doi.org/10.1016/j.peptides.2015.04.012 Burnette...protein A. Analytical Biochemistry 112:195–203 . https://doi.org/10.1016/0003-2697(81)90281-5 Pillai-Kastoori...the user, for the user. J Biol Chem 295:926–939 . https://doi.org/10.1074/jbc.ra119.010472 Renart J, Reiser...the National Academy of Sciences 76:3116–3120 . https://doi.org/10.1073/pnas.76.7.3116 Towbin H, Staehelin...the National Academy of Sciences 76:4350–4354 . https://doi.org/10.1073/pnas.76.9.4350 Yang P-C, Mahmood...and trouble shooting. North Am J Med Sci 4:429 . https://doi.org/10.4103/1947-2714.100998 Additional resources...

  6. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type
    Blog Post
    Published
    Sept. 14, 2021, 1:15 p.m.
    ...plasmid in Escherichia coli Stbl3. 3 Biotech 3:61–70. https://doi.org/10.1007/s13205-012-0070-8   Chakiath ...reduced-genomeEscherichia coli. BioTechniques 43:466–470. https://doi.org/10.2144/000112585   Ding T, Huang C, Liang...editing in Escherichia coli. Microb Cell Fact 19. https://doi.org/10.1186/s12934-020-01321-4   Kolodner ... the National Academy of Sciences 77:4847–4851. https://doi.org/10.1073/pnas.77.8.4847 Trepotec Z, Geiger...translation efficiency or half-life. RNA 25:507–518. https://doi.org/10.1261/rna.069286.118 Additional resources...

  7. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    Published
    May 28, 2024, 1:45 p.m.
    ... I excluded here for better visibility. Source: https://www.bdbiosciences.com/en-us/resources/bd-spectrum-viewer...relation to the laser type. Table adapted from: https://emea.bd.com/panel-design/en/flow-cytometry-fluochrome-brightness-spillover...depending on the laser light's wavelength. Source: https://www.bdbiosciences.com/en-us/resources/bd-spectrum-viewer... at higher wavelengths of about 750 nm. Source: https://www.bdbiosciences.com/en-us/resources/bd-spectrum-viewer...cannot use them together in the same panel. Source: https://www.bdbiosciences.com/en-us/resources/bd-spectrum-viewer...fluorophores used in our panel (Table 2). Source: https://www.bdbiosciences.com/en-us/resources/bd-spectrum-viewer...

  8. Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

    Type
    Blog Post
    Published
    Nov. 20, 2018, 8:17 p.m.
    ...identifier format for lenti-Cas9-VQR is the URL “http://n2t.net/addgene:87155” where the Addgene registered... as “RRID:Addgene_87155” and the following URL (https://scicrunch.org/resolver/RRID:Addgene_87155) will...gift from Daniel Bauer (Addgene plasmid # 87155 ; http://n2t.net/addgene:87155 ; RRID:Addgene_87155).” ...gift from Daniel Bauer (Addgene plasmid # 87155 ; http://n2t.net/addgene:87155 ; RRID:Addgene_87155)” Cite... gift from Daniel Bauer (Addgene plasmid # 87155 http://n2t.net/addgene:87155)” Cite plasmid using RRID...

  9. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    Published
    Aug. 4, 2020, 1:15 p.m.
    ...observational study. The Lancet Microbe 1:e14–e23 . https://doi.org/10.1016/s2666-5247(20)30004-5 McCray PB...Respiratory Syndrome Coronavirus. JVI 81:813–821 . https://doi.org/10.1128/jvi.02012-06 Ou X, Liu Y, Lei ...cross-reactivity with SARS-CoV. Nat Commun 11: . https://doi.org/10.1038/s41467-020-15562-9 Subbarao K,...model for SARS? Trends in Microbiology 14:299–303 . https://doi.org/10.1016/j.tim.2006.05.007 (2020) Open ...Open for outbreaks. Nat Biotechnol 38:377–377. https://doi.org/10.1038/s41587-020-0499-y Additional resources...

  10. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    Published
    Oct. 19, 2023, 1:15 p.m.
    ...Transgene Expression. Hum Gene Ther, 31(3–4), 151–162. https://doi.org/10.1089/hum.2019.274. PMID: 31914802. ... System. Hum Gene Ther Methods, 28(5), 277–289. https://doi.org/10.1089/hgtb.2016.133. PMID: 28967288....capsid protein modification. Front Med, 10, 1106085. https://doi.org/10.3389/fmed.2023.1106085. PMID: 36817775...Adeno-Associated Virus. Hum Gene Ther Methods, 30(6), 206–213. https://doi.org/10.1089/hgtb.2019.276. PMID: 31752513....increases transgene expression. Sci Rep, 7(1), 5432. https://doi.org/10.1038/s41598-017-04054-4. PMID: 28710345...
Showing: 71 - 80 of 242 results