We narrowed to 855 results for: NTS

...are too few resources to teach students how to perform these experiments in the beginning. In most classrooms...lab publication [1] so that the students performing these experiments have a good baseline for comparison...techniques such as basic observation and dissections. Students don’t usually perform more modern techniques used...in genetics or molecular biology because the experiments are either not accessible or too challenging ...easier to perform advanced developmental biology experiments in planarians with their recent plasmid deposit...deposit. These plasmids enable two main types of experiments: Whole mount in Situ Hybridization (WISH) for... expression. The 49 deposited plasmids enable students to study gene expression and functions of key ...

... sequence in between two fragments of EGFP in pCAG-EGxxFP. The EGFP fragments contain 482bp of overlapping...between two non-fluorescent split GFP fragments. When the two fragments, expressed in the presynaptic region...genome. They did this by selecting the important elements from existing transposon and vector systems and...an all-synthetic vector that included only the elements needed for function. The lab validated this vector..., particular for those involving recombination elements such as FRT or Lox sites, and provides a modular...system for easier exchange and re-use of existing elements. DNA sequences for a desired component are cloned... Gate cloning step to assemble the individual components into a destination vector in a predefined order...

... reliable reagents for future research. I’ve talked to many researchers who have complaints about the ...provide you with additional validated reagents, and, with these reagents in hand you’ve got a good foundation...tries 5 separate experiments at once to get confirmatory data for a hypothesis and presents only the results... Scientific reproducibility As Jeffrey Flier points out in his talk (1:24 - 29:31), there are a variety...Freedman et al 2015). Finally, as Pamela Hines of AAAS points out on the panel, irreproducibility undermines ... 1: Inability to repeat previously performed experiments Researchers should be able to follow one another...another’s protocols and use one another’s reagents with the confidence that they aren’t simply doing an ...

...60.2%) 20 graduate students (25.6%) 12 masters students (15.4%) 46 (59%) of these students were in the last...-program self-assessment, participants completed follow-up self assessments and program evaluations every...self-assesment. Achievements directly attributable to participation in the program included improvements in writing... got a physical award, all the students experienced great improvements, not necessarily quantifiable, ...mentorship during undergraduate and graduate studies. Students also need more access to reputable, organized ... networks connecting international experts and students. In response to such needs, the International ... BWH and providing career mentoring to several students from Spain and all over the world, she thought...

...particular, two recent events have the potential to influence both graduate students and postdocs broadly... Board to allow graduate students who work as teaching or research assistants to join unions. The topic...example, Yale graduate students filed for union elections in only 10 departments across the university....flexibility to address the unique needs of students from different departments across the university and alterations...advocacy skills “bootcamp” for a focused group of participants. More information can be found at http://futureofresearch.org...F1000Research, 2014; Dolan, F100Research, 2016). These events have also started progress towards solutions. The...which several projects were initiated. Recent developments promise to further change the landscape facing...

...works in the lab knows how expensive tiny amounts of reagents are, how complicated (and again, expensive...FD-CF pellets are easy for students to use in the classroom because the students simply need to add water...Most importantly, experiments for FD-CF reactions can be fun and engaging for students. In our BioBits ...example, we’ve designed FD-CF experiments that result in outputs that students can easily sense, whether ...consider using some elements of BioBits to transition your undergraduate students from the classroom to...had this kind of experience earlier! After all, students build circuits in physics class to learn about...funding and the resources to provide them to their students. My desire to create a set of low-cost, easy-to-use...

...minimal upfront requirements and allows you to expediently join multiple synthetic fragments, create multiple...refers to a method of physically joining multiple fragments of DNA to create a synthetically designed DNA ...multiple mutations in one or multiple fragments, and generate constructs for producing single-guide RNAs... yielding scarless DNA assembly, and allowing fragments to be taken directly from restriction enzyme digests...the absolute end of a fragment. This means that fragments (containing regions of homology) cut from plasmids...planning. This cloning methodology requires that the fragments to be joined contain regions of homology at, or...homology can be contained within the individual fragments themselves, but doesn’t necessarily have to be...

...well treatments are working to fight the cancer.  Beyond early modifications to standard treatments and...are running out of hope with more traditional treatments, such as surgery, radiation, and chemotherapy...sufferers can see great promise in up and coming treatments like immunotherapy. Mesothelioma Mesothelioma...and late discovery mean that most mesothelioma patients have an extremely poor prognosis. Together, the...disease with only about 3,000 new mesothelioma patients diagnosed every year in the US. Therefore, mesothelioma...many forms of cancer, the primary conventional treatments for mesothelioma include surgery to remove cancerous...cases the cancer is diagnosed so late that these treatments do not work very well. The best option in such...

...directly as therapeutic agents or serve as vehicles for delivering other treatments, their efficacy can be...library of mutants and how they are screened. When conducting antibody evolution experiments, you’ll typically...bioengineering is antibody engineering, to improve reagents and therapeutics. What is antibody engineering...tools are, the better the reproducibility of experiments and the validity of conclusions. Antibody engineering...allows for more efficient and targeted delivery of treatments, ultimately increasing treatment effectiveness...chimeric antibodies, and smaller engineered antibody fragments. One technique we haven’t discussed in depth is...including chimerization, conjugation, and antibody fragments. Fab: fragment antigen binding; Fc: fragment crystallizable...

...failure to clearly articulate one's talents and ideas. Some key points from my ebook, "Applying for a Faculty...success is publications and grants. 2. Write succinct and easy to understand documents A common misconception...example documents. This part has generated a lot of positive feedback. Many of these documents are too...sections, along with new thoughts on research statements. Snapp, who is always looking for ways to improve...improve his eBook, adds: "If you have any questions, comments or suggestions, please do not hesitate to email... members will NOT be in your area. Therefore, documents need to minimize jargon. Equally importantly, ...seminar See item 2. Attendees will include grad students (that are solicited for feedback), possibly the...