...Biology. New York: W. H. Freeman, 2000. Bookshelf ID: NBK21668. Matsuno-Yagi, Akemi, and Yasuo Mukohata... about FLEx vectors Download our Plasmids 101 eBook Read our plasmids blog posts Resources on the Addgene...molecular biology reference Find Cre-lox plasmids at Addgene Browse plasmids at Addgene ...

...high-performing antibodies for Synaptotagmin-1 (Uniprot ID P21579) for use in western blot, immunoprecipitation... (tagged or untagged) protein When expression plasmids are available, or the necessary expertise to clone...involves the transient transfection of expression plasmids in a given cell line. For flow cytometry, it is...

... (A) Cumulative CRISPR plasmids shipped by year. (B) Cumulative CRISPR plasmids deposited with Addgene...,000 CRISPR plasmids in our repository, and we have shared almost 300,000 CRISPR plasmids and 12,000 CRISPR...these CRISPR plasmids (and libraries)? We wanted to know, so we classified CRISPR plasmids containing a...CRISPR plasmids requested each year by year of deposit (Figure 3), we saw that once CRISPR plasmids become... CRISPR plasmids seem to never truly go out of style. Instead, an almost even mix of plasmids (by age)...Total CRISPR plasmids deposited by country; square size is proportional to number of plasmids deposited ...Total CRISPR plasmids requested by country; square size is proportional to the number of plasmids requested...

...rectangles) are cloned into various plasmids using oligonucleotides. These plasmids contain Type IIS restriction...Golden Gate/Gibson Assembly Multiplexing Plasmids: These plasmids allow you to assemble 2-4 gRNAs through...Golden Gate/Gibson Assembly Multiplexing Plasmids: These plasmids can be used to express up to 8 gRNAs after...Yang Lab Single Transcript Multiplexing Plasmids: These plasmids are similar to the Csy4 polycistronic ...Chen Lab Golden Gate Assembly Multiplex Plasmids: These plasmids allow expression of 2-5 gRNAs in zebrafish...tools to help you multiplex - we’ll use mammalian plasmids to introduce you to some of your potential options...and cloning methods, but please scroll down for plasmids suitable for other model systems, including E....

...received over 13,000 plasmids requests for COVID-19 related plasmids. These plasmids have reached labs in...small collection of plasmids for coronavirus research, including a handful of plasmids that have been in...some highlights of the plasmids shared over the last year and how these plasmids have been used to accelerate...circulating and plasmids containing these mutations have been deposited at Addgene. Plasmids deposited from... labs also deposited plasmids for producing COVID-19 diagnostic enzymes. Plasmids in this toolkit include...These plasmids contain both untagged and tagged versions of SARS-CoV-2 ORFs and include plasmids that ... has now grown into a collection of over 2,400 plasmids, with many available to scientists in industry...

...sequencing all incoming plasmids. If you are interested in requesting one of these plasmids, you can request ...data for each of their plasmids, from descriptions of key components of the plasmids to partial, full, or...the identified differences, the plasmids are sent out to Plasmidsaurus for long-read nanopore sequencing...amplification and manipulation to produce and edit plasmids. These are prone to error — both human and biological...especially shared ones.  The importance of QC Plasmids are a fundamental part of research. They are easy...cloning process — including the propagation of plasmids in bacteria — has many points at which small errors...what was originally intended. Sharing unverified plasmids can lead to far reaching consequences, from wasting...

...associated plasmids with Addgene. Since then, Addgene has filled over 6,500 requests for these plasmids. While...those containing plasmids that the authors deposited with Addgene and those without plasmids deposited with...2,596 of which had plasmids deposited with Addgene. Articles with Addgene plasmids get more citations ...with plasmids deposited at Addgene – typically about four times as many as papers without plasmids deposited...Papers without Addgene Plasmids in Journal (E) Percent of Papers with Addgene Plasmids in Journal (F) Weight...get cited more?   Articles with and without plasmids at Addgene To find out whether scientific articles...journals from 2005 to 2015 in areas most relevant to plasmids: Genetics & Heredity, Cell Biology, Biotechnology...

...inducible inactivation. Find the Robinson plasmids or the Royle plasmids and get to work! Robinson et al...mGRASP plasmids. Additionally, the lab recently described the use of another set of mGRASP plasmids in their... know what plasmids you would like to seen on our Hot Plasmid list. Other popular plasmids & blog posts...sgRNA libraries expand upon the CRISPR family of plasmids by offering a lentivirus-based mechanism for sgRNA...new CRISPR screening tools, see our CRISPR/Cas Plasmids: Pooled Libraries webpage or read our blog post...resources at www.addgene.org/CRISPR/ to browse CRISPR plasmids, watch informational videos, download protocols... with extremely-dim brightness, bfloGFPc1. The plasmids used for determining the structures are now available...

...contains all the plasmids from the same lab while the publication page contains all the plasmids deposited from...as ccdB survival (for Gateway plasmids).  We also like to store plasmids that need extra stability, such...construct by the depositing lab. There may be other plasmids with similar names in the collection, but you ...The flame that appears beside some of Addgene’s plasmids is an indication of popularity. A yellow flame...blue, more than 100 times! When you search for plasmids on our website, one of the available filters popularity... popularity (e.g. only showing those plasmids which have been ordered more than 100 times).     I guess... pages is relatively new, so some of our older plasmids may not have an entry in this field. If you’ve...

...system plasmids are available through Addgene and are already very popular. Use these plasmids to increase...deposited a pair of plasmids for the cotranslational incorporation of unnatural amino acids (UAAs) into proteins...reactions, fluorescent amino acids, posttranslationally modified amino acids, photoaffinity probes, and ... can visualize: Cell Structure Plasmids: Cell Structure Plasmids: ER mCherry-Sec61 beta BFP-Sec61... our community of depositors. With over 40,000 plasmids avaliable in the repository, we can't give all...mediated genome modification and gene expression. The plasmids associated with this article can be obtained through...Addgene: Rapamycin-inducible Cas9 sets (Addgene plasmids 62883 &62884; 62885 & 62886) Rapamycin-inducible...

...All plasmids deposited at Addgene enhance scientific sharing worldwide but a select few are particularly...particularly special. Over 1000 of our 60,000+ deposited plasmids have been requested more than 100 times each, ...to sharing epitomized by the creators of these plasmids with the first annual Blue Flame Awards. What'...scientists around the world choose to share their plasmids through our repository since we know biological...Addgene has transformed the way in which we share plasmids and other molecular tools with the scientific ...throughout the research community. These popular plasmids are categorized into a 3-level system on our website...depositing PI’s who have the most highly requested plasmids. Currently, there are roughbly 400 depositors ...

...transfecting four different plasmids into the producer cells (two packaging plasmids, an envelope plasmid, ...transfer plasmids replication competent? What is SIN? A6: Most (if not all) Addgene transfer plasmids are ...Addgene's most popular lentiviral plasmids Overview of Lentiviral Packaging Plasmids Lentiviral Protocols and ...Addgene has an extensive collection of lentiviral plasmids created for a variety of applications including...I package lentivirus with retroviral packaging plasmids (and vice versa)? A1: Lentiviruses are part of...actually work? That depends. The majority of transfer plasmids use the weak viral LTR promoter to drive expression...available here). 2nd vs. 3rd Generation Lentiviral Plasmids Q3: How can you tell 2nd- and 3rd-generation transfer...

...cloning vector. Lentiviral Plasmids -  If you're familiar with the pLKO plasmids you'll note that those are...Actually, these two plasmids from Didier Trono's lab are our two most popular plasmids of the past 10 years...influx of plasmids for reprogramming differentiated cells into pluripotent stem cells. Plasmids from Shinya... have 19 different ubiquitin plasmids in the repository. 100,000 Plasmids Shipped! - By 2010, Addgene ...with all the new plasmids. So far, we have distributed over 10,000 CRISPR/Cas9 plasmids to more than 3,...time, Addgene has shipped over 350,000 individual plasmids to 5,000 different research institutions. This...2009 was ubiquitin. The most requested ubiquitin plasmids were pRK5-HA-Ubiquitin-WT and HA-Ubiquitin, and...

...organizations have made their plasmids available beyond academia. For example: Plasmids from the Michael J. Fox...and plasmids from several labs at Florida State University became openly available. Find plasmids available...one critical part was missing when the COVID-19 plasmids first became available: distribution was limited...universities to make COVID-19 research-related plasmids available more broadly: to scientists in academia...unprecedented amount of support about allowing use of plasmids for research by anyone. As infection rates were...scientific community speed research is to offer plasmids created as part of research projects, another ...up with Ginkgo Bioworks to quickly bring these plasmids to the scientific community - both academic and...

...; Wu, Asokan, et al., 2006). AAV2 capsids are available as plasmids in the repository and can be selected...; Wu, Asokan, et al., 2006). AAV8 capsids are available as plasmids in the repository, as part of our ... true! In a previous post, we covered systemic capsids, which allow AAVs to broadly spread throughout ...BioRender.com. AAV vectors have both distinctive capsids and genomic structures. The viral capsid is the...your choice of promoter/enhancer. Table 1: AAV capsids and receptors. Capsid Primary receptor Coreceptor...Coreceptor AAV1 α2,3-/α2,6 N-linked sialic acids1 AAV receptor2   AAV2 Heparan sulfate proteoglycan...nomenclature (AAV1, AAV2, etc.) to distinguish the capsids from one another. Common serotypes We’ll cover...

... to make plasmids that already exist, and we significantly reduce costs of producing plasmids with high-throughput...several plasmids in parallel. That way, you can run one PCR, one gel, etc. for multiple plasmids at once...about. When we were closing in on the one million plasmids shared milestone in 2018, we wrote a post on the...a plasmid. In 2023 (and at closer to 2 million plasmids shared than not!), we think it’s about time we...Institute has created hundreds of thousands of plasmids, using both mass production genome wide screens...explore to maximize the use of both when making plasmids. Use repositories Repositories such as Addgene...consumables, and time. We also handle the MTA, QC the plasmids, provide helpful resources, and, on the rare occasion...

...restriction-modification systems onto plasmids allowed cell populations to retain more of these plasmids as compared to those..., the unstabilized plasmids were retained in less than 10% of cells while plasmids containing the plasmid...efficient cloning Read all Plasmids 101 blog posts Read about knockout/knock-in plasmids Resources on Addgene.org... antitoxin to avoid cell death. In the case of plasmids, toxin-antitoxin systems act as “selfish” pieces...associated genes. These systems better ensure that plasmids are passed down through generations (Figure 1)...addiction systems” require dividing cells to retain plasmids - if a cell does not inherit plasmid due to improper...of DNA, the plasmid was propagated better than plasmids without this DNA. They eventually designated this...

...lentiviral plasmids Addgene's most popular retroviral plasmids Addgene's collection of AAV plasmids Addgene...while the other plasmids with packaging or replication components are called helper plasmids. Below, we’ll...For safety and production reasons, multiple plasmids with many components are used to package lab grown...of these vectors are also split across several plasmids (often three), for safety purposes. The primary...evolution. There are 1st, 2nd, and 3rd generation plasmids for their production. As such, there are bonus.... Rep is required for viral replication and also aids in infrequent genome integration at the AAVS1 locus...vectors Addgene has many viral vectors and helper plasmids for all the viruses discussed above (gamma-retrovirus...

... cloned into plasmids to investigate for each of these planarian species. These plasmids all have a PR-T4...Resources at Addgene.org Find plasmids from the Sánchez lab Find plasmids for other developmental models...planarians with their recent plasmid deposit. These plasmids enable two main types of experiments: Whole mount...of specific gene expression. The 49 deposited plasmids enable students to study gene expression and functions...publication [1] from the Sánchez lab describes the plasmids and protocols required for both WISH and RNAi ...materials and protocol resources. Find Sánchez lab Plasmids Visualizing gene expression via Whole Mount In... the desired plasmid backbone. The Sánchez lab plasmids allow students and new labs to bypass these steps...

...have deposited their plasmids into the Addgene repository. More than 4,500 plasmids have already been deposited...in research, I developed several new plasmids, including: plasmids to study the dimerization of HLA B27... ready to answer your questions about Addgene, plasmids, and depositing (all while our US counterparts...Since 2004, Addgene has shipped more than 75,000 plasmids to more than 1,500 different institutions in Europe...Europe. In the last four years alone, the number of plasmids distributed to Europe has doubled going from 9,500...to more than 20,000 in 2013. And the number of plasmids requested by scientists based in Europe over the...Resonance Energy Transfer (BRET) technique, and plasmids to study the expression of an unknown protein ...