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We narrowed to 513 results for: EMB

Showing: 211 - 240 of 513 results

  1. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    Published
    Sept. 3, 2020, 12:15 p.m.
    ... Biology 22:169–174 . https://doi.org/10.1016/j.chembiol.2014.12.011 Polstein LR, Gersbach CA (2015) A...Chem Biol 11:198–200 . https://doi.org/10.1038/nchembio.1753 Richter F, Fonfara I, Bouazza B, Schumacher...Chem Biol 13:443–448 . https://doi.org/10.1021/acschembio.7b00603 Additional Resources “Optogenetics Meets...

  2. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ...1), which may be easier to conceptualize. Just remember to switch them back before reporting the data!...use it! Tempting as it may be to skip this step, remember we here at Addgene frown upon statistical laxness...11237753. Primer design resources PrimerBlastDINAmelMfoldBLASTEnsembl More resources on the Addgene blog...

  3. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    Published
    Sept. 28, 2020, noon
    ...are easily inserted using BbsI and Golden Gate Assembly. Either plasmid can be also linearized with XbaI...elements, like a repair template, using Gibson Assembly or restriction enzyme cloning. Streptomyces bacteria...Microbiome Bacteria with a Toolkit for Modular Assembly of Broad-Host-Range Plasmids. ACS Synth Biol 7...

  4. Lucky Thirteen: Guest Contributors of 2023

    Type
    Blog Post
    Published
    Dec. 5, 2023, 2:15 p.m.
    ...It's December, and that means it's officially the start of Addgene's winter holiday celebrations! We'...with Addgene writers High Complexity Golden Gate Assembly with NEB's DADExperts: Matthew Norton and NEBGCE4All...

  5. SciComm with the Experts at Science in the News Part 1

    Type
    Blog Post
    Published
    March 16, 2017, 1:06 p.m.
    ... former Co-director of Science in the News, and member of the SITN podcast team.     Amy Gilson recently...former Co-director of Science in the News, and is a member of the SITN podcast team. You can follow Amy on...

  6. Depositor Week 2020 is Happening Now. Join us!

    Type
    Blog Post
    Published
    Nov. 16, 2020, 2:15 p.m.
    ...Mark your calendars for November 16-20, 2020 for #DepositorWeek on Twitter!  That’s today through Friday...presented in this blog post includes data until November 9, 2020. Between then and the posting of the blog...

  7. Celebrating One Million Plasmids Shared!

    Type
    Blog Post
    Published
    Sept. 25, 2018, 2:39 p.m.
    ... that everything will ship out in time - from assembling the boxes, to inoculating tubes, and to packing...pic.twitter.com/eJbI0lLiGQ — Dr. Joanne Kamens (@JKamens) September 13, 2018 This massive scale reagent sharing led...

  10. MassBioEd Career Exploration Day at Addgene

    Type
    Blog Post
    Published
    Nov. 13, 2015, 3:30 p.m.
    ...intellectual property from our Tech Transfer team members Tony Cova and Chiara DeNatale and programming with...great European photo slideshow from our UK team member Benoit Giquel. Bluegene’s globetrotting impressed...

  12. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    Published
    March 11, 2014, 5:55 p.m.
    ...are coming along at a fast and furious pace. In December, as we've blogged about before, there was the ...via Cas9/RNA-Mediated Gene Targeting in One-Cell Embryos. Niu Y, et al. Cell. 2014 January 30. (Pubmed) ...

  13. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    Published
    March 21, 2019, 12:47 p.m.
    ...genome editing plasmids String assembly gRNA cloning (STAgR) String assembly gRNA cloning (STAgR), developed...

  15. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    Published
    March 22, 2016, 2:30 p.m.
    ...to specific drugs in specific contexts, so he assembled a plasmid kit designed to do exactly that. As ... described in the journal Science Signaling in December 2014, he and his colleagues at Duke University...

  16. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    Published
    Oct. 31, 2019, 1:06 p.m.
    ...algae cell is just the cell wall and cytoplasmic membrane. Vampirovibrio chlorellavorus will not grown in...appropriately named as spook, spookier, phantom, disembodied, shadow, and shade. Tongue-eating louse Cymothoa...

  19. SciComm with the Experts at Science in the News Part 2

    Type
    Blog Post
    Published
    April 27, 2017, 2:30 p.m.
    ... former Co-director of Science in the News, and member of the SITN podcast team. You can follow Vini on...former Co-director of Science in the News, and is a member of the SITN podcast team. You can follow Amy on...

  21. Inside Addgene's 10 Year Anniversary Gala

    Type
    Blog Post
    Published
    July 3, 2014, 3:48 p.m.
    ...all of the Addgene employees, the Addgene board members, vendors, Addgene supporters, and of course, Executive...integral part in its success. The Addgene board members were also recognized for their essential advice...

  22. Three Tips to Organize Your Lab Notebooks in the New Year

    Type
    Blog Post
    Published
    Jan. 2, 2014, 3:44 p.m.
    ...filled with family, food, and travel, and you can’t remember where you put your flow cytometry protocol. Or...Tip 3: Share protocols and spreadsheets with lab members Now that you’ve organized your protocols, wouldn...

  23. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    Published
    June 2, 2022, 2:15 p.m.
    ...participate in a brief fifteen minute interview. I remember leaving the interview hopeful to continue on in...the beginning, I spent a lot of time shadowing members of my team to learn what I was to do for my time...

  26. Sensing Neuronal Dopamine

    Type
    Blog Post
    Published
    July 30, 2019, 12:59 p.m.
    ...performed with a probe surrounded by a semipermeable membrane, which is inserted into the region of the brain...passive diffusion of DA across the semipermeable membrane from the extracellular fluid into the perfused...

  27. An Addgene Summer Internship

    Type
    Blog Post
    Published
    Aug. 8, 2024, 7:22 p.m.
    ...for the first time, being introduced to all the members around the office one by one, I knew that I belonged...right concentration of a reagent during our HiFi assembly but decided to go ahead anyway. We were running...

  28. Year of the Camelid: Antibody Style

    Type
    Blog Post
    Published
    May 9, 2024, 1:15 p.m.
    ...The UN General Assembly has declared 2024 the International Year of Camelids. The declaration is intended...Llamas Required - Synthetic Nanobodies Against Membrane Proteins More resources from addgene.org  Plasmids...

  29. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    Published
    Sept. 15, 2016, 2:30 p.m.
    ...came to visit us and watch fellow Run4Rare team members complete the Boston Marathon, he wanted to run ..., the R206H mutation makes ACVR1 responsive to members of the ubiquitous activin ligand family, including...

  30. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    Published
    Nov. 25, 2013, 10:12 p.m.
    ...destroy the invader. Wikipedia, accessed 25 November 2013. Author: James Atmos (2). Many CRISPR/...(12): 5429–33. Photo: Wikipedia, accessed 25 November 2013. Author: James Atmos. http://en.wikipedia.org...
Showing: 211 - 240 of 513 results