Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 573 results for: BASE

Showing: 351 - 400 of 573 results

  1. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ...popular counterselection vector is the pDS132 sacB-based allelic exchange vector. This vector contains sacB...expression in Escherichia coli using a rhamnose-based tightly regulated and tunable promoter system." ...

  3. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    Published
    Jan. 4, 2022, 2:15 p.m.
    ...either “personal” or “substantive”. A personality-based conflict occurs when two people cannot agree or ...describes 5 basic approaches to conflict resolution based on various levels of assertiveness and cooperation...

  4. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    Published
    Feb. 18, 2016, 3:42 p.m.
    ...Since the number of base pairs for each varies, it is difficult to calculate this based on DNA concentration...

  5. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    Published
    Nov. 7, 2014, 2:56 p.m.
    ...their primary use in the lab. These strains are all based on E. coli K-12 and are considered BSL-1. Table ...potential to recombine. This strain is endA+, so column-based DNA preps require an additional wash step. F- glnV44...

  6. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    Published
    Jan. 11, 2017, 8:47 p.m.
    ...variety of new tools came out including the single base editors, Casilio, CombiGEM, and a variety of pooled...

  7. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    Published
    Dec. 29, 2020, 2:15 p.m.
    ... kit. 3. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas by Shravanti Suresh Guest blogger... these tools for plasmid mapping, DNA/protein databases, DNA sequence manipulation, and primer design....

  8. Top 10 Open Science Developments of 2013

    Type
    Blog Post
    Published
    Dec. 19, 2013, 3:46 p.m.
    ...reviews on an agent used to promote bone growth based on data voluntarily made available by Medtronic ...organization called Free the Data launched a public database to gather the results of Myriad's genetic tests...

  13. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    Published
    May 23, 2023, 1:15 p.m.
    ... protein tag to the target and using an epitope-based strategy such as Anti-6xHis. You could also consider...L. (2021). Creating clear and informative image-based figures for scientific publications. PLOS Biology...

  14. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    Published
    May 31, 2016, 3:30 p.m.
    ...frequency of ‘TTAA’ in the genome is 1 in every 256 base-pairs of DNA sequence, making it very useful for...This allows researchers to avoid making unnecessary base pair changes as would be the case with ssODNs. One...

  18. Science as Art

    Type
    Blog Post
    Published
    March 2, 2015, 3:13 p.m.
    ...Daniel Stoupin is a PhD scientist and photographer based at the University of Queensland, whose outstanding...

  21. SunTag and Fluorescent Imaging

    Type
    Blog Post
    Published
    March 28, 2017, 2:30 p.m.
    ...fluorescence, SunTag can also be used to improve CRISPR-based activation of target genes, but we’ll save that ...

  28. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    Published
    Aug. 11, 2015, 2:30 p.m.
    ...chromatin organization. This fluorescent CRISPR/Cas9-based method is a great step forward in live cell imaging...

  30. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    Published
    July 11, 2017, 2:30 p.m.
    ...Listed - a CRISPR Screen Tool Green Listed is a web-based software tool freely accessible though http://greenlisted.cmm.ki.se...: PMC4486245. 4. Ma, Hongming, et al. "A CRISPR-based screen identifies genes essential for West-Nile-...

  31. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ...non-enveloped viruses, so they are resistant to alcohol-based disinfectants. A 10% bleach solution should be used...interested in using chemogenetic and optogenetic AAV-based tools to study neural control. Surprisingly, little...

  33. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    Published
    July 6, 2021, 1:28 p.m.
    ...Transposable elements are split into two classes based on their mechanism of transposition: Class I TEs...Guide RNA-Assisted Targeting) system. INTEGRATE is based on a naturally occurring Tn7 transposon found in...

  39. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    Published
    April 2, 2020, 6:17 p.m.
    ..., etc). The cloning method is ultimately chosen based on the plasmid you want to clone into. Regardless...

  40. Year of the Camelid: Antibody Style

    Type
    Blog Post
    Published
    May 9, 2024, 1:15 p.m.
    ...lab and into the clinic as well. The first sdAb based therapeutic was approved in 2018, with many others...

  41. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    Published
    May 18, 2023, 1:15 p.m.
    ... cellular phagocytosis for effective macrophage-based cancer immunotherapy.Sci. Adv.8,eabl9171(2022).DOI...

  42. Hot Plasmids - October 2022

    Type
    Blog Post
    Published
    Oct. 6, 2022, 1:15 p.m.
    ... genetically-encoded voltage indicators (GEVIs) based on the ASAP sensors. The resultant JEDI-2P sensors...

  43. Summer SciComm: Science Storytelling

    Type
    Blog Post
    Published
    Sept. 16, 2022, 1:15 p.m.
    ...audience can understand the conflict. A science-based conflict gives you an easy framework with which ...

  44. RUBY-Red Siliques

    Type
    Blog Post
    Published
    March 1, 2022, 2:15 p.m.
    ...Transformation CRISPR 101: CRISPR-Mediated Plant Base Editors...
Showing: 351 - 400 of 573 results