We narrowed to 619 results for: LIS

...infect both non-dividing and dividing cells. They accomplish this through nuclear localization signals which...on the Addgene Blog Learn More about Lentivirus Listen to Connie Cepko Discuss Gene Therapy with AAV Figure...

...Finally, as previously mentioned, enlist the help of your network to polish the final draft prior to submission...

... from this collection in your experiments and published your data, please fill out this form and we’ll...complete QC and you can find all available plasmids listed on Ginkgo’s collection page.  Bacterial expression...

...plasmid carrying the required gene.  The table below lists some of the most commonly used selection markers...said element. This link provides a more extensive list of yeast auxotrophic markers and includes the associated...

...group of mentees to get more “bang for the buck”. Listen to our podcast interview with Harvard Medical School... the internet, but start by downloading my handy list of suggestions.  Get started. Have regular meetings...

...was frustrated by the difficulty of accessing published plasmids from other scientists. She, Kenneth Fan...others could request from — Addgene!  Addgene was established as an e-commerce company, which helped it stay...

...tissues. Although antibody production is well established, it’s also a process that’s difficult for individual...their RANbodies, Yamagata et al used previously published nanobody sequences that targeted the following...

...incorrect; take it instead as valuable feedback.  Listen. Think about the questions you’ve received, read...read the comments, seek feedback from others, and listen quietly as people discuss their experiences. If...

...affinity reagents.    Types of Affinity Reagents  (Listed in no particular order.) Fab fragments are created...distinction of being the only affinity reagent on this list derived from nature. Single-domain antibodies are...

...cytometry’s standard approach is the direct method. ELISAs, immunohistochemistry, and immunocytochemistry ...Introduction to Immunofluorescence Antibodies 101: ELISAs Antibodies 101: Introduction to Antibodies Resources...

...BASU, is derived from a BirA* homolog found in B. subtilis. BASU was  compared head-to-head with the E. coli...IREB2, which post-transcriptionally regulate iron metabolism pathway transcripts. By using RaPID, Ramanathan...

...point, decide early on what to do in a namespace collision. It may even be worth it to have extra columns...solving, but consistency is key. Some namespace collisions you may encounter: vector, sequence, insert, ...

...consider—we have loaded those we are aware of in our checklist for tissue and cell research. Using cells from...of this literature in our health and medicine checklist. A common worry for researchers wanting to consider...

...experiments The first thing you will need is to get the list of effective gRNA sequences targeting your genes...pool. (Adapted from Wong et al., 2016). With a list of gRNA targeting sequences, you can then readily...

... a very big way. In one of his latest papers, published in Molecular Systems Biology in November, Voigt...(2014).   We talked with Voigt about this collision that’s taking place between CRISPR technology ...

... I use Cre-lox? The Cre/lox system is a well-established research tool, especially in the field of mouse... transgenics. A few of the most common uses are listed below. Cre-dependent gene expression – placing...

...for the administrative side of the grant to be established. Take time to read and fully understand the RFA...achieving each aim. A funded proposal typically lists the deliverables, explains 1-2 potential pitfalls...

...possess plasmid-borne toxins like colicins and subtilisins to kill other bacteria in the area, freeing up...Ruscheweyh, H.-J., Sunagawa, S., Mclellan, S. L., Willis, A. D., Comstock, L. E., & Eren, A. M. (2024). ...

...but so is where you’re building it! Table 1 has a list of Fragmid destination vectors currently available... you to fill in module fragments from a dropdown list for every module. Both the modules and the components...

...at the Faculty of Sciences of NOVA University in Lisbon, Portugal. She leads the Glycoimmunology group ...approved by the FDA in 1939 for the treatment of botulism and Lambert-Eaton myasthenic syndrome (LEMS) because...

...tool that you are looking for and check our curated list of biosensors too, as another laboratory may have...designed to measure intracellular calcium and published in 1997. Since that time, numerous advances in...

...surprised by how multi-purpose it can be. Let’s review a list of MoClo applications. Genetic circuits Most things... many different MoClo kits have already been established. Keep an eye out for our next blog on choosing...

... an increase in the complexity. In 2018, they published a paper profiling four base overhang ligation ...fragments may be near the upper limit of what can be accomplished with Golden Gate Assembly—even when getting ...

...up clear tasks for each member of the team to accomplish so that many elements of the project were able...Addgene Blog Read other Synthetic Biology Posts Listen to Our Interview with Previous Collin's Lab Member...

...annotation for 90 bacterial elements. By Ppgardne at English Wikipedia, CC BY-SA 3.0, https://commons.wikimedia.org...both polyadenylation and termination. Out of those listed, the SV40 late polyA and rbGlob polyA are thought...

... In that case, an RQ value of one (or, more realistically, between 0.80-1.20) would mean you have one ... Curr Protoc Hum Genet. 2014;80:7.21.1-7.21.. Published 2014 Jan 21. doi:10.1002/0471142905.hg0721s80 ...

... PCR was invented in the early 1980s by Kary B. Mullis who later shared a Nobel Prize in Chemistry for...PCR is that it can amplify DNA using only a short list of reagents and several heating and cooling steps...

...vectors? Viral genome sequencing Thanks to the establishment of in-house sequencing capabilities and Seqwell...not map to the reference sequence. The resulting list of hits is then carefully reviewed. As mentioned...

...Western blots. ELISAs. Immunofluorescence. What do all of these techniques have in common? They all typically...not better, than antibodies for all of the above listed molecular techniques, but they can also be expressed...

... to reflect upon before they attempt to make essentialist connections between biology and ancestral groups...the long and ugly history of reporting false essentialist links between biology and ancestry group as ...

...The tradeoffs between various cloning methods are listed in Table 2. For example,  chaining-based cloning...used in various chaining-based cloning methods are listed in Table 3. The goal of high throughput cloning...

...Originally published Nov 30, 2017 and updated Jul 31, 2020. Cas13 enzymes are quickly becoming major ...for RNA editing. Two years after that, the lab published a paper on an RNA editor that allows C to U edits...

...examines the inherent brokenness of scientific publishing and research funding. I’ve been aware of these... place. Can we address problems in scientific publishing without acknowledging the systemic sexism in ...

...stay up to date and engaged with new advances in published science. They also serve as a way to learn about...blog posts centered around scientific papers and publishing Find tips for leading a great meeting ...

...classic, net-neutral dye scaffold due to its established chemistry, small size, brightness, and cell permeability...In past decades, further refinements to this established dye structure have yielded commercial panels ...

...our educational resources. From 2013–2023, we published 110 videos, which received over two million views...across the globe?” By asking this question, and by listening to the answers from our community, we’ll be able...

...to a job that requires ddPCR skills. Your resume lists ddPCR, so the hiring manager knows that you have...experience in [field of research], and have excellent [list the most important skills for the position]. I am...

...be time-consuming or even very frequent, but establishing a few minutes every month dedicated to thinking...Originals how non-conformists move the world. Penguin Publishing Group. Day, F. (2019). Embrace your weird face...

...transduction was not detected. They also used an ELISA to measure binding of PHP.B to a soluble version... See Supplementary Table 3 in Huang et al. for a list of PHP.B permissive and non-permissive mouse strains...

...polling some Addgene colleagues, we’ve come up with a list of things to consider as you make this transition...careers and desk jobs “alternative careers.” Realistically, based on the numbers of PhDs and available ...

...Andreas Rett, who tried to raise awareness and published his observations in a German medical journal. ...in mice is possible (Guy et al., 2007). They accomplished this using Cre-Lox mouse models in which the...

...Originally published Aug 30, 2018 and updated April 16, 2020. Sensitive and specific nucleic acid detection...SARS-CoV-2 using a lateral flow strip format. They published this in Nature Biotechnology. Like the SHERLOCK...

... what institutions are actually doing (using the list of 340 institutions with postdocs in science, engineering... attempted to check 123 of the 340 institutions listed as having postdocs, representing ~85% of the postdoctoral...

...develop communication strategies, or understand, and publish on the health and economic benefits of novel drugs...Experiment with New Careers while in Grad School Listen to Michael Koeris' Journey from Grad Student to...

...properly? We suggest starting by using the newly published oxidative optimized palette of FPs (moxFPs) [5... using moxFPs over standard FPs for the reasons listed above. In addition, because the moxFPs are highly...

...a particular sequence. Browse Addgene's curated list of Bacterial Expression Systems Table 1: E. coli...better at 30°C or room-temperature, which is accomplished by growing your culture to the desired density...

...most used pairs to measure FRET. The table below lists plasmids that can be used to create your choice ...measurement methods as feasible when first beginning to establish the FRET methodology for a given experiment. You...

...valuable tool to study cellular physiology and metabolism under various conditions. LiLac has improved ...capable of local temperature sensing with sub-millisecond resolution and sub-degree temperature differences...

...especially if the options outlined above aren’t realistic. Check Out Our Post on Sequencing Options for ...like CRISPRitz or CRISPOR can help narrow down the list of sites where off target editing may occur, allowing...

...in eukaryotic cells as an intermediate of heme metabolism (Shemetov et al, 2017). Modern state-of-the-art...protein expression level. State-of-the-art FPs (listed in Table 1) were optimized for efficient binding...