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Addgene
Showing: 351 - 400 of 697 results

  1. Inside Addgene's 10 Year Anniversary Gala

    Type
    Blog Post
    Published
    July 3, 2014, 3:48 p.m.
    ...the last 10 years as well as the many people that make up the Addgene family. It was great to see the faces...Just as her grandfather used the printing press to make copies of the newspaper, Addgene uses E. coli bacteria...

  2. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    Published
    June 2, 2022, 2:15 p.m.
    ... needed to go to an appointment at 3 PM, I could make up the hours I had missed at another time. This ...case for me at Addgene. There is a clear effort to make sure people feel connected.    As I became accustomed...

  3. Scientific Sharing in the Time of COVID-19: Databases and Resources

    Type
    Blog Post
    Published
    May 12, 2020, 1:15 p.m.
    ...servers and in journals, and researchers have been making new materials like plasmids available to scientists...as the best way that the scientific community can make progress towards understanding this disease. ...

  4. 2023 and 2024: Reflections and Looking Ahead

    Type
    Blog Post
    Published
    Jan. 23, 2024, 2:15 p.m.
    ...look, and a reorganization of existing content to make it more accessible. Other accessibility updates ...typically adopted by for-profit companies, while making use of our nonprofit status to achieve mission-...

  5. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    Published
    Sept. 29, 2015, 2:30 p.m.
    ...pSiM24, can also be used with biolistic methods, making it useful for engineering plants that are not susceptible...available pCAMBIA and pKYLX vectors, respectively, making it ideal for large gene transfer. The M24 promoter...

  6. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    Published
    Aug. 11, 2015, 2:30 p.m.
    ...Cas9’s ease of use and ability to label live cells make this system truly advantageous. This new technique...altered in disease states. dCas9 gets a colorful makeover Even without its nuclease activity, catalytically...

  7. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    Published
    July 9, 2015, 2:30 p.m.
    ...: if there is no place to upload a cover letter, make your cover letter the first page of your resume ... don't ask, you will never get the help that can make a difference. Having your resume forwarded personally...

  10. London Calling - Addgene’s New Europe Office in London

    Type
    Blog Post
    Published
    June 10, 2014, 3:20 p.m.
    ...interacting with people and talking about science makes her the perfect Addgenie and she can’t wait to see...even visit your lab! We look forward to helping you make some serious discoveries.   10 Years of Plasmid...

  11. Antibodies 101: Affinity Tags

    Type
    Blog Post
    Published
    Sept. 19, 2023, 1 p.m.
    ... a little thoughtful planning, affinity tags can make protein purification a cinch. Types of affinity ...that is unlikely to perturb protein function. This makes it amenable to being left on your protein of interest...

  14. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    Published
    Sept. 15, 2016, 2:30 p.m.
    ...in healthy patients. However, the R206H mutation makes ACVR1 responsive to members of the ubiquitous activin...awareness of FOP. Please consider running with us or making a donation via the link below. Please continue ...

  16. Management for Scientists: Managing vs. Leading

    Type
    Blog Post
    Published
    March 31, 2015, 4:08 p.m.
    ...in doer mode even late in our careers.  Managers make sure the work gets done Managers do this by removing...communication. A manager must both hire and fire to make sure the team is the best it can be. Managers must...

  18. 10 Ways to Share Your Science!

    Type
    Blog Post
    Published
    May 13, 2014, 8:34 p.m.
    ... to share your science 1)   If you don’t already make it a habit to talk – really talk – about the research...science, they have good advice for anyone looking to make their science matter. If you aren’t ready for a ...

  19. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    Published
    March 13, 2014, 4:08 p.m.
    ...and numerous experimental design decisions to make, it makes sense that scientists, many of whom are venturing...

  20. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    Published
    Jan. 30, 2014, 3:29 p.m.
    ...scientists rarely stop to think about how much easier it makes our lives. Plasmid transformation into E. coli is... strains have natural antibiotic resistances, so make sure your plasmid and E. coli strain are compatible...

  21. Choosing a Good Mentor for Scientists

    Type
    Blog Post
    Published
    Jan. 16, 2014, 2:56 p.m.
    ...serve as a good mentor. To read more about what makes a good mentor, see the previous post in this series...posts in our Science Mentors series: Part 1: "What Makes a Good Mentor?" and 6 More FAQs About Science Mentoring...

  23. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    Published
    Sept. 26, 2017, 12:12 p.m.
    ...stop for refreshment along the way? What ecosystem makes your heart sing with delight? From developmental...other scientists, or science journalists, or policy makers. The term editor covers a wide variety of careers...

  24. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    Published
    Oct. 27, 2016, 2:30 p.m.
    ...extra extension time after the last cycle of PCR to make sure that the "A" gets added to all PCR products... the standard ligation reaction is fairly quick, make sure you stay organized and prepare everything you...

  25. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    Published
    July 21, 2022, 1:15 p.m.
    ...whirlwind of skill acquisition. Classes covered filmmaking, podcasting, philosophy of science, creative ...misinformation in the wake of a global pandemic can make for a sore reminder of how far we have yet to go...

  26. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    Published
    June 9, 2022, 1:15 p.m.
    ...science communication research.   This summer, we’re making time to talk about my favorite thing - science ...but the foundation of all good communication is making sure everyone’s on the same page. So for our first...

  27. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type
    Blog Post
    Published
    Sept. 17, 2020, 1:15 p.m.
    ...was awed by the teamwork and initiative it took to make all this happen with 80% of the team working remotely...Protection Program loan and received enough funds to make most of our payroll for the spring and early summer...

  28. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    Published
    April 25, 2019, 3:01 p.m.
    ...Biotechnology that now can be deposited at Addgene. What makes these fluorescent proteins unique and what can they...excitation at 493 nm and max emission at 509 nm), making it useful in multicolor labeling particularly with...

  29. Plasmid Cloning by PCR

    Type
    Blog Post
    Published
    March 29, 2016, 2:30 p.m.
    ...In its simplest form, PCR based cloning is about making a copy of a piece of DNA and at the same time adding...restriction site (GAATTC) to the 5’ end of this primer, making our Forward Primer 5'-GAATTCATGTGGCATATCTCGAAGTAC...

  30. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type
    Blog Post
    Published
    Sept. 15, 2015, 2:30 p.m.
    ...from the Rare Genomics Institute and Addgene will make a substantial difference in pushing the work forward...that with this award from Addgene, we’ll be able to make big progress in elucidating association between ...

  31. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    Published
    Aug. 21, 2014, 3:15 p.m.
    ...?” and "What type of science careers would would make a good fit for me?" Whatever would I do next and...for sales and marketing jobs Like most choices we make, my job choices were mostly by chance. Luckily, ...

  32. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    Published
    Oct. 19, 2023, 1:15 p.m.
    ...terminal repeats (ITRs). ITRs are the elements that make an AAV plasmid an AAV plasmid.  Within the AAV ...and disruptive mutations and truncations that can make vector propagation and sequencing a headache. How...

  33. Cultivating Community Science at BosLab

    Type
    Blog Post
    Published
    Nov. 2, 2017, 1:35 p.m.
    ...working in our laboratory! Don’t worry though, we make sure that everyone gets proper equipment and safety...engineer yeast to do similar things. The focus is to make yeast engineering accessible to everyone but these...

  34. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    Published
    April 18, 2019, 12:53 p.m.
    ...tools out there to visualize genomic loci, what makes CRISPR-Sirius unique? Unlike fluorescent in situ...predecessor CRISPRainbow, CRISPR-Sirius is brighter making it suitable for observing low-copy genomic loci...

  36. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    Published
    Sept. 6, 2017, 1:01 p.m.
    ...Our Plasmids 101 eBook! One of the features that makes NEBuilder HiFi so accurate (apart from the high-...restriction enzyme digests to DNA assembly. What makes it so forgiving, is the incorporation of a 3’-5’...

  37. Addgene Depositors Get More Citations

    Type
    Blog Post
    Published
    April 20, 2017, 2:30 p.m.
    ...published in the same journals as those that did make deposits to Addgene.  Insomuch as different journals... argument that Addgene is achieving its goal of making plasmid-based science more accessible…and accruing...

  38. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    Published
    June 6, 2023, 1:15 p.m.
    ...then inserted into the host's genome and is used to make more virus. The most studied (and used in the lab...also transduce dividing and non-dividing cells, making it conducive to targeting all cell types. Altogether...

  39. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ... Thus, the adjustable nature of viral libraries makes them the ideal vector for the job. Therapeutic ...immunogenicity, and cell-specific targeting features make them an ideal delivery system for human patients...

  40. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    Published
    April 5, 2022, 1:20 p.m.
    ...     The Trimmer Lab has continued to work to make the sequences of monoclonal antibodies publicly ...sequences from the Trimmer lab hybridoma collection and make them publicly available. The lab has also expanded...

  42. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    Published
    Sept. 26, 2024, 1:15 p.m.
    ...Sequence- and ligation-independent cloning: A SLIC makeover In 2007, LIC received an important update, courtesy...for multi-fragment DNA assembly, and its low cost makes it ideal for researchers doing large amounts of ...

  43. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    Published
    June 6, 2024, 1:50 p.m.
    ...control for a specific marker in your experiment. To make an FMO sample, you take a subset of your experimental... is prepared very similarly to a FMO sample: you make a sample from a combined subset of your experimental...

  44. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    Published
    May 26, 2020, 1:15 p.m.
    ...If you’re using CRISPR to make a genome edit, how do you know if your CRISPR experiment was successful...different constructs for the reporter to work and to make the desired genome edit:  A donor plasmid that ...

  45. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    Published
    Jan. 28, 2020, 2:35 p.m.
    ...of their optimally suited temperature ranges and make them grow better at suboptimal ones, which could...within a regular mesophilic host system. This would make the host able to grow at low temperatures suitable...

  46. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ...and molecular biologists use to tinker with DNA. Making changes to a DNA sequence can help scientists identify... are a few drawbacks with these approaches that makes it challenging for users to achieve targeted diversification...

  48. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    Published
    July 22, 2014, 5:46 p.m.
    ...testing and a lack of well-characterized genetic tools make it challenging to engineer a specific function in...construct our color changing flower we are carefully making our way through the IP landscape, using plant parts...

  49. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ...systemically administered in an organism. These features make AAV ideal for human therapies and in vivo laboratory...delivery of genetic cargo to a host cell. In order to make room for a gene of interest, AAVs used in research...

  50. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    Published
    Feb. 16, 2023, 7:05 p.m.
    ...suppression of expression is faster and easier than making a knockout and is a great way to temporarily reduce...integrated (once or many times) into the genome. Make sure to choose an appropriate promoter to express...
Showing: 351 - 400 of 697 results