We narrowed to 481 results for: mme
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TypeBlog PostPublishedMay 9, 2017, 2:12 p.m....thoughts about Cpf1 multiplexing, leave them in the comments below. References 1. Zetsche et al. "Multiplex...
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Communicating Your Science With Help From ComSciCon
TypeBlog PostPublishedJuly 19, 2018, 1:12 p.m.... it's not always recognized and valued for its immense importance, it may well be what determines whether... -
Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers
TypeBlog PostPublishedJuly 28, 2015, 2:30 p.m....percentage of the proteins inside a cell have a commercial antibody against them. There’s also a question... -
Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs
TypeBlog PostPublishedJune 2, 2015, 6:06 p.m....thoughts about this technology, leave them in the comments below. References Boosting CRISPR/Cas9 multiplex... -
Form Your Own Peer Mentoring Group: A How-To Guide for Scientists
TypeBlog PostPublishedFeb. 18, 2014, 4:22 p.m.... you involved in Peer Mentoring relationships? Comment below with ideas from your own mentoring group ... -
Will You Be My Mentor? Finding and Asking for Mentoring Support
TypeBlog PostPublishedFeb. 4, 2014, 3:50 p.m....and make sure to choose one that comes highly recommended from someone you trust. A good coach can make... -
Kiran Musunuru on the Newest TALEN Genome-Editing System
TypeBlog PostPublishedDec. 10, 2013, 3:13 p.m....the DNA-binding domain is modifiable and can be programmed to bind to essentially any DNA sequence desired... -
Antibodies 101: Fab Fragments
TypeBlog PostPublishedSept. 5, 2024, 1:15 p.m....Imagine an antibody. Do you immediately visualize a Y-shaped protein reminiscent of the Addgene mascot... -
Plasmids 101: The protein expression toolbox
TypeBlog PostPublishedFeb. 16, 2023, 7:05 p.m....References References Nabet et al. The dTAG system for immediate and target-specific protein degradation. Nature... -
CRISPR 101: Cas9 vs. The Other Cas(s)
TypeBlog PostPublishedDec. 8, 2022, 2:15 p.m....Lucas, Burstein, David, Chen, S., Janice, et. al. Programmed DNA destruction by miniature CRISPR-Cas14 enzymes... -
Antibodies 101: Secondary Antibodies
TypeBlog PostPublishedDec. 21, 2021, 2:15 p.m....blot can be run in a single day, I really cannot recommend it except in the most dire of circumstances.) ... -
What's New in CRISPR - November 2021
TypeBlog PostPublishedNov. 18, 2021, 2:15 p.m....Nature Biotechnology Find the Cas12f-GE plasmids Programmed genome editing by a miniature CRISPR-Cas12f nuclease... -
It’s Bioelectric! An Exciting Interdisciplinary Field of Research
TypeBlog PostPublishedOct. 29, 2019, 2:52 p.m.... al., 2011). Additionally, researchers have reprogrammed regenerating tissues without using genome engineering... -
Troubleshooting Your Plasmid Cloning Experiment
TypeBlog PostPublishedSept. 24, 2019, 12:55 p.m....major companies selling PCR reagents. Nowadays commercial polymerases are very efficient, but if you are... -
Human Germline Editing Using CRISPR
TypeBlog PostPublishedAug. 10, 2017, 2:19 p.m....probably seen this study (and various articles and commentaries) pop up on Twitter and Facebook, shared by biologists... -
Lighting Up Cell Signaling with Photoswitchable Kinases
TypeBlog PostPublishedJuly 25, 2017, 1:20 p.m....downstream steps. It’s also possible to determine immediate effects of signaling vs the delayed effects because... -
Choosing the B(right)est Fluorescent Protein: Aggregation Tendency
TypeBlog PostPublishedJune 15, 2017, 2:30 p.m....monomer in the cell type tested. To conclude, we recommend that you try a couple of different fluorescent... -
Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number
TypeBlog PostPublishedSept. 4, 2018, 12:53 p.m....protein as the repressor because TALEs can be programmed to bind tightly to arbitrary DNA sequences and... -
Addgene's Tips for Plasmid Quality Control
TypeBlog PostPublishedJan. 14, 2016, 3:30 p.m....Tell us your favorite sequencing tricks in the comments section below. Additional Resources on the Addgene... -
The Future of Research Symposium Boston 2015
TypeBlog PostPublishedOct. 20, 2015, 2:30 p.m....hired to produce results, not scientists” These comments are well correlated with published data. For example... -
Plasmids 101: Golden Gate Cloning
TypeBlog PostPublishedAug. 27, 2015, 2:30 p.m....Gate assembly is also less expensive than many commercial cloning methods. Golden Gate and synthetic biology... -
Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing
TypeBlog PostPublishedMay 5, 2015, 3:54 p.m....searching, reading and organization tips in the comments below. Additionally, read more of the Beginner... -
Career Coaching for Scientists: Why and Where Do I Find One?
TypeBlog PostPublishedJan. 29, 2015, 1:25 p.m....consider. Feel free to add other suggestions in the comments or email me with suggestions. Most coaches have... -
Hot Plasmids February 2024
TypeBlog PostPublishedFeb. 20, 2024, 2:45 p.m....which are also available in trial sizes. Addgene recommends these recombinant antibodies for use in western... -
CRISPR in the Clinic
TypeBlog PostPublishedNov. 2, 2023, 1:15 p.m.... edited healthy donor cells can be delivered immediately, saving precious time. CRISPR can aid in editing... -
Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)
TypeBlog PostPublishedAug. 24, 2021, 1:15 p.m....direct and indirect ELISA. Sandwich ELISA Most commercially available ELISA kits are for a sandwich ELISA... -
Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene
TypeBlog PostPublishedApril 6, 2021, 12:30 p.m.... about our sequencing results in the Depositor Comments section at the bottom of the plasmid page. Viral... -
A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells
TypeBlog PostPublishedMay 7, 2020, 1:15 p.m....potential therapeutic approaches, the authors recommend mRNA delivery, the V106W version of ABE8, and ... -
Early Career Researcher Toolbox: Finding Relevant Papers
TypeBlog PostPublishedJan. 9, 2020, 1:45 p.m....tip Checking the number of retweets, likes, and comments on a tweet about a paper can help you gauge how... -
Plasmids for Endogenous Gene Tagging in Human Cells
TypeBlog PostPublishedApril 6, 2017, 1:02 p.m....relying instead on microhomology-mediated end-joining (MMEJ). To find out more about gene edited hiPS cell lines... -
A Guide to Designing a Scientific Poster: Content, Layout, and More
TypeBlog PostPublishedOct. 22, 2019, 12:20 p.m....spectators can read the text from many feet away and immediately understand the point of the work. The other poster... -
Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors
TypeBlog PostPublishedAug. 20, 2019, 12:31 p.m....interfere with certain tests and is not always recommended. Environmental influences like bright light ... -
Science communication: 9 Strategies to Get Your Foot in the Door
TypeBlog PostPublishedSept. 27, 2017, 1:23 p.m.... Science. Don’t be scared to engage with them. Comment on their activities and participate in discussions... -
Pathways Over Time Plasmids Engage Students in Functional Genomics Research
TypeBlog PostPublishedMay 22, 2018, 1:38 p.m....Met protein complementation. In the procedure diagrammed in Figure 3, students first transform the S. ... -
Plasmids 101: Terminators and PolyA signals
TypeBlog PostPublishedMarch 31, 2016, 2:30 p.m....however, any common terminator will suffice. Many commercial expression vectors use double terminators to ... -
Tips for Titering Your Lentiviral Preps
TypeBlog PostPublishedMarch 15, 2016, 2:30 p.m....straightforward and rapid as there are several commercially available p24 ELISA kits that can quantify p24... -
Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015
TypeBlog PostPublishedDec. 1, 2015, 3:30 p.m....DNA which has a boring structure.” That certainly summed up this conference—nucleic acid science still has... -
Illuminating Epigenetics with A FRET Based Biosensor
TypeBlog PostPublishedNov. 19, 2015, 3:30 p.m....126.19 (2004): 5982-5983. PubMed PMID: 15137760. Recommended further reading and viewing The Epigenetics ... -
Experimenting in an Open Source lab: from CRISPR to Cats
TypeBlog PostPublishedJuly 23, 2015, 2:30 p.m.... woodworking tools, and a metalshop. After two summers in Silicon Valley as a student and then instructor... -
How-to: 5 Steps to a Great Panel Discussion
TypeBlog PostPublishedOct. 22, 2014, 1:57 p.m....stays on schedule. People can only sit so long. I recommend no more than 40 minutes for the panel discussion... -
Hot Plasmids: Winter 2025
TypeBlog PostPublishedMarch 11, 2025, 1:15 p.m....antibodies targeting the human genes and are recommended for use in immunohistochemistry (Figure 5). Anti-MAPT... -
Common Injection Routes in Mice
TypeBlog PostPublishedJan. 22, 2025, 9:42 p.m.... mice are under anesthesia, but they are not recommended for tumorigenic compounds, as circulation is ... -
Production of Virus in Insect Versus Mammalian Cells
TypeBlog PostPublishedNov. 5, 2024, 8:32 p.m.... are standard but can take seven to 10 days. Commercially available kits provide titers in just two days... -
Magnetic Control of Proteins: More than a Dream
TypeBlog PostPublishedSept. 24, 2024, 1:15 p.m....Dodson, C. A., Wallace, M. I., Mackenzie, S. R., Timmel, C. R., & Hore, P. J. (2016). Chemical amplification... -
Antibodies 101: Flow Compensation
TypeBlog PostPublishedMay 16, 2024, 1:15 p.m....starting to use flow cytometry, it’s generally recommended to start compensating with single-color controls... -
Plasmids 101: Degron Tags
TypeBlog PostPublishedMarch 14, 2024, 1:15 p.m....Paulk J, Dastjerdi S, et al. The dTAG system for immediate and target-specific protein degradation. Nat Chem... -
Polymerase Chain Reaction Overview and Applications
TypeBlog PostPublishedAug. 10, 2021, 1:15 p.m....manually in water baths but is now easily done in a programmed thermocycler. For more details on this procedure... -
xCas9: Engineering a CRISPR Variant with PAM Flexibility
TypeBlog PostPublishedMarch 28, 2018, 6:52 p.m....let us know how xCas9 is working for you in the comments section! References Hu, Johnny H., et al. "Evolved... -
3D Printing Meets CRISPR Cas9
TypeBlog PostPublishedOct. 17, 2017, 12:55 p.m....family-run business – 3D Molecular Designs – to commercialize some of the best instructional materials that... -
Truncated gRNAs for Regulating Gene Expression
TypeBlog PostPublishedJan. 10, 2017, 3:37 p.m....making targeted modifications to DNA, Cas9 can be reprogrammed to serve as a regulator of gene expression. ...