We narrowed to 488 results for: mme

...ladder system is a cost-effective alternative to commercial ladders which also provides a useful yet low-...

...have another short "quiet period" instead of immediately running to the next thing.  It may seem counterintuitive...

..., Bestagno M, Burrone O, Maynard J, Ferrara F, Trimmer JS, Görnemann J, Glanville J, Wolf P, Frenzel A...

... appropriate isotype controls linked on their commercial antibody pages. Flow cytometry can be a complicated...

...yield dsDNA. Unlike LTRs, ITRs are perfectly symmetrical to allow for this pairing to occur. Like LTRs...

...sometimes cofactors) to be provided and are generally dimmer than fluorescent proteins. On the other hand, they...

...could be useful in your research? We definitely recommend checking out GCE4All’s website and resources, ...

...emerging viral applications and give you some recommendations for putting viruses to work. Viral applications...

...before a study is conducted. Editors and reviewers recommend changes, and if everyone agrees that the research...

...percentage of the proteins inside a cell have a commercial antibody against them. There’s also a question...

...thoughts about this technology, leave them in the comments below.  References Boosting CRISPR/Cas9 multiplex...

... you involved in Peer Mentoring relationships? Comment below with ideas from your own mentoring group ...

...and make sure to choose one that comes highly recommended from someone you trust. A good coach can make...

...the DNA-binding domain is modifiable and can be programmed to bind to essentially any DNA sequence desired...

...protein as the repressor because TALEs can be programmed to bind tightly to arbitrary DNA sequences and...

...probably seen this study (and various articles and commentaries) pop up on Twitter and Facebook, shared by biologists...

...downstream steps. It’s also possible to determine immediate effects of signaling vs the delayed effects because...

...monomer in the cell type tested. To conclude, we recommend that you try a couple of different fluorescent...

... al., 2011).  Additionally, researchers have reprogrammed regenerating tissues without using genome engineering...

...major companies selling PCR reagents. Nowadays commercial polymerases are very efficient, but if you are...

...Lucas, Burstein, David, Chen, S., Janice, et. al. Programmed DNA destruction by miniature CRISPR-Cas14 enzymes...

...blot can be run in a single day, I really cannot recommend it except in the most dire of circumstances.) ...

...Nature Biotechnology Find the Cas12f-GE plasmids Programmed genome editing by a miniature CRISPR-Cas12f nuclease...

...Imagine an antibody. Do you immediately visualize a Y-shaped protein reminiscent of the Addgene mascot...

...References References Nabet et al. The dTAG system for immediate and target-specific protein degradation. Nature...

...Tell us your favorite sequencing tricks in the comments section below.  Additional Resources on the Addgene...

...hired to produce results, not scientists”  These comments are well correlated with published data. For example...

...Gate assembly is also less expensive than many commercial cloning methods. Golden Gate and synthetic biology...

...searching, reading and organization tips in the comments below. Additionally, read more of the Beginner...

...consider. Feel free to add other suggestions in the comments or email me with suggestions.  Most coaches have...

...potential therapeutic approaches, the authors recommend mRNA delivery, the V106W version of ABE8, and ...

...Met protein complementation. In the procedure diagrammed in Figure 3, students first transform the S. ...

...relying instead on microhomology-mediated end-joining (MMEJ). To find out more about gene edited hiPS cell lines...

...spectators can read the text from many feet away and immediately understand the point of the work. The other poster...

...interfere with certain tests and is not always recommended.  Environmental influences like bright light ...

...direct and indirect ELISA.   Sandwich ELISA Most commercially available ELISA kits are for a sandwich ELISA...

...which are also available in trial sizes. Addgene recommends these recombinant antibodies for use in western...

... edited healthy donor cells can be delivered immediately, saving precious time. CRISPR can aid in editing...

... about our sequencing results in the Depositor Comments section at the bottom of the plasmid page. Viral...

...tip Checking the number of retweets, likes, and comments on a tweet about a paper can help you gauge how...

... Science. Don’t be scared to engage with them. Comment on their activities and participate in discussions...

...however, any common terminator will suffice. Many commercial expression vectors use double terminators to ...

...straightforward and rapid as there are several commercially available p24 ELISA kits that can quantify p24...

...DNA which has a boring structure.” That certainly summed up this conference—nucleic acid science still has...

...126.19 (2004): 5982-5983. PubMed PMID: 15137760. Recommended further reading and viewing The Epigenetics ...

... woodworking tools, and a metalshop. After two summers in Silicon Valley as a student and then instructor...

...stays on schedule. People can only sit so long. I recommend no more than 40 minutes for the panel discussion...

...let us know how xCas9 is working for you in the comments section! References Hu, Johnny H., et al. "Evolved...

... in a new and creative way? Let us know in the comments section below! References 1. Terskikh, Alexey...

...making targeted modifications to DNA, Cas9 can be reprogrammed to serve as a regulator of gene expression. ...