We narrowed to 582 results for: BASE;

...Reporter signals may be electrochemical or light-based, with luminescent and fluorescent reporters being... domain induces changes in a fluorescent signal based on ligand presence or changing cellular conditions...Method Notes Quantification of a process Plate-based assays (plate reader) Detect the average amount...

...repository for DNA-based materials, including our popular kits and libraries. This DNA-based materials repository...

...initiating a base editor-based clinical trial for SCD in 2022 and Editas Medicine starting a Cas12-based clinical... today. Below we will review some of the CRISPR-based editing therapies that are currently approved or...

...called GMAP (Gibson assembly-based modular assembly platform). GMAP is based on the Gibson Assembly method... Cas9-based AID tagging Seven years ago, Masato Kanemaki's lab successfully used the plant-based auxin-inducible...University, we are now providing a powerful CRISPR-based screening source for the Drosophila community as... it is composed of two sub-pools. The first is a base “90k library” that contains ~90,000 gRNAs that target... biosensor A pH-sensitive fluorescent biosensor-based assay system was recently deposited with Addgene...University of Zagreb, constructed a CRISPR-Cas9-based tool for targeting CpG methylation by fusing catalytically...proteins in a target cell. In addition to nucleotide-based linking strategies, post translational protein fusion...

...robust protocol it does have limitations. The enzyme-based CUT&RUN method overcomes many of these limitations...interacting. The CUT&RUN method Enter CUT&RUN, an enzyme-based protocol for identifying DNA binding patterns that...sets, of which there are many readily available. Based on the success that our lab and others have had ...

...from some labs have developed fluorescent-protein based DA sensors (Figure 2). The dLight1 dopamine sensor... dLight1 sensor is a single fluorescent protein-based sensor. This sensor was engineered from a GFP variant...sensor Yulong Li’s lab has created the GRABDA sensor based on a similar strategy: a GFP variant bound to a ...

... or other products in the suite. Since it’s web-based, you could access it from anywhere. Its ease of ...Biorender is like clip art for scientists. The web-based collection of over 20,000 icons is designed by professional...stop struggling to make figures in PPT 🔬 Also, the base version is free, forever 💜 https://t.co/KJpyxgxeQt...

... level with CellTag CellTagging is a lentiviral based tagging method that labels cells with heritable ...three-fold increase of fully reprogrammed cells based on single-cell gene expression patterns. The lab...Chuner, et al. "CellTag Indexing: genetic barcode-based sample multiplexing for single-cell genomics." bioRxiv...

...desktop-based software for plasmid design and mapping. ApE (A plasmid Editor): A free, donation-based plasmid...knowledge resource for cell lines IBEX Knowledge Base: A data repository for validation data on IBEX reagents...antibodies: A database of validated antibodies for mouse, human, and marmoset brain IHC.  FPbase: A data repository... Flame plasmid pET21a-BirA.   DNA / protein database search and analysis tools Addgene's analyze sequence...sites Open reading frame finder  Addgene vector database: Find sequences for plasmids assembled from publications...nucleotide or protein sequences to sequences in a database ExPASy translation tool: Translates a DNA sequence...sequence using all six open reading frames UniProt: A database of protein sequence and functional information...

...School, he developed the first lentiviral microRNA-based system for polymerase II-regulated RNA interference...virology,” he says. He was working in an HIV lab and based on that experience, soon directed himself to finding...months, the lab began a project to develop an AAV-based COVID-19 vaccine.  Vandenberghe’s work with AAVs...

...polymerase, the construction of specific sequences for base pair overhangs, insert and vector purification, ...specific to the insert with an additional 15-17 bases that are complementary to the overlapping vector...introduced into my inserts. However, with any PCR-based cloning technique there is a chance of introducing...

...tells us about a new tool for efficient multiplex base editing and multiplex prime editing (requiring minimal...dCas9 (with no modifications!) as a CRISPR/dCas9-based tool for DNA demethylation. AAV Q&A with Tim Miles...

...therapeutic potential. Note: constructs discussed are based on Adenovirus type 5. Three generations of adenoviral...delivery: Potential applications for gene and cell-based therapies in the new era of personalized medicine...formation assay on A549 cells . Alternatively, antibody-based assays are commercially available for faster detection...

...short ncRNAs, such as microRNAs that are around 20 base pairs long. Using a single guide RNA (sgRNA) to ...Figure 1: A schematic illustration of CRISPR/Cas9-based approaches to study ncRNAs. (A) Transcription start...your experiments, there are plenty of other CRISPR-based options that can target ncRNAs.  CRISPRi and CRISPRa...10.1126/science.1225829  Hazan J, Bester AC. CRISPR-Based Approaches for the High-Throughput Characterization...

...American, Africa, or South Asian) used in the screens based on the cells’ germline genetic variations. They ...generating genetic dependency data with an ancestry-based bias.   The correction process does not stop there...genome-wide screens, introducing bias into all work based on these and any other CRISPR screens using the ...variation contributes to false negatives in CRISPR-based experiments with varying burden across ancestries...Cancer Dependency Map (DepMap), a cancer dependency database used to make this information accessible to the...

...packaging systems have been developed over the years based on HIV-1; however, the 1st-generation vectors are...defines 2nd- or 3rd-generation transfer vectors based on whether or not they have a chimeric 5'LTR. Second-generation...? What percent of the vector components are HIV-based? A7: No single plasmid contains all the components...insertional mutagenesis. These risks are largely based upon the vector system used and the transgene insert...

...plexWell™ Library Preparation Technology for NGS-based sequencing and confirmation of Addgene’s large and...plexWell™ workflow also offers a highly efficient NGS-based alternative to Sanger sequencing because the sample... To date, this has resulted in high quality NGS-based assemblies for over 3000 plasmids in the Addgene...

...While there are more and more molecular biology-based activities making their way into high school classrooms...BioBits ExplorerTM and BioBits BrightTM, biology kits based on freeze-dried, cell-free (FD-CF) components. Making...and is interested in developing synthetic biology based tools for science outreach and advocacy. You can...

...community.     Why recombinant antibodies? Animal-based methods for antibody production have several drawbacks..., 2015a, b). Recombinant antibodies are plasmid-based and offer numerous advantages, including more secure...Antibodies 101 blog post Learn more about plasmid-based recombinant monoclonal antibodies Resources on ...

...and jargon Hemingway Editor  This is a free web-based tool for detecting the readability of your writing...or dissertations. Phrases are categorized either based on the section of a manuscript they might be found...to work with collaborators. Zotero is a desktop based program that’s compatible with Mac, Windows, and...directly import papers from web pages and academic databases.  Downloading Mendeley Cite lets you cite papers...