...microbiome by using engineered genetic tools and reporter microbes. Let’s take a look at a few of these ...is a ribosome binding site (RBS) followed by a reporter gene. The hairpin blocks translation by sequestering... reveals the RBS and allows translation of the reporter gene resulting in GFP signal. With the addition....org Check out our microbiology resource page Deposit microbiology plasmids with Addgene Find synthetic...

...getting support from depositors. We regularly hear how much they see the value in depositing and they are increasingly... own labs get excited about making their first deposit to Addgene. We are also lauded by another set...large community of scientists who support us by depositing, requesting and spreading the word. Our blog...

...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles...articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to ...you'd ever like to write about a recent plasmid deposit please sign up here.        AAV delivery of split...indicator plasmids at Addgene! Beck et al., Sci Report 2019. PubMed PMID: 31685893. A MoClo toolkit for...

...the source or were deposited when the now-published article was a preprint. Depositing plasmids that were... great way to do that. We are happy to receive deposits of plasmids that have been described in a preprint... authors can include the Addgene ID for their deposited plasmids in the final journal publication or an...

...experiments in planarians with their recent plasmid deposit. These plasmids enable two main types of experiments...expression of specific gene expression. The 49 deposited plasmids enable students to study gene expression... genes (all of which are available through the deposit) are described in the Sánchez lab publication [...different planarian genes, we encourage you to deposit them back with Addgene and expand the number of...

...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles...articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to ...you'd ever like to write about a recent plasmid deposit please sign up here.     Listen to the Hot Plasmids...future work with this goal, the Barrick lab has deposited the pDCAF3-IS5 plasmid containing a synthetic ...

...scientist. New to the bench? Fragmid depositor John Doench reports even the greenest members of his lab...modular cloning approach, it’s easy to switch out reporters, Cas proteins, guide cassettes, promoters, N’ ...your own Fragmid component (which you can also deposit with Addgene!) We’re excited to help the Doench...

... plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles.  Here's ...PAGE editing with viral sgRNA targeting mCherry reporter (mChe), with co-incubation with assist peptide.... CAP-Mac also robustly expressed fluorescent reporters in green monkeys and cultured human neurons, with...Arokiaraj (Gradinaru lab) Another useful viral tool deposited by the Gradinaru lab, AAV1-X1, was developed by...

...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to ...you'd ever like to write about a recent plasmid deposit please sign up here.  Here's what you'll find in...they could not proliferate. Eastman et al. Cell Reports 2020. https://doi.org/10.1016/j.celrep.2020.107804...

...GCaMP6 family of sensors are commonly used calcium reporters with different kinetic parameters. There are three...also available. Detection methods Many calcium reporters use a single fluorescent protein (FP), requiring...unwanted variables. Twitch is a FRET-based calcium reporter derived from troponin C. It is engineered with...for in vivo studies) There are several calcium reporters specially designed for the aims and requirements...

... plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles.  Here's what...2024 under a CC-BY 4.0 license.  The team deposited an array of constructs for tagging the kinesins...Hao and Lee et al., 2024. The authors have deposited AAV plasmids for Cre-dependent and -independent...6E10-A5] in Immunohistochemistry (Human). Addgene Report, doi: https://doi.org/10.57733/addgene.6ncijt.  ...

...Fluorescent Protein (GFP/EGFP) for a transcriptional reporter or as a general cytoplasmic label of cells, there's...FP dimerization. Equally problematic, several reportedly monomeric FPs are not actually monomeric, at ...Incorporation into a FRET biosensor A FRET biosensor is a reporter that undergoes a conformational change to bring...help you make better fluorescent fusion protein reporters for your studies and greatly lower your chances...

...expression plasmid. Experimental use Since the initial report describing CRISPR-mediated purification of a specific...including Protein A, 1xFLAG, 2xAM, and HA have been reported, as well as affinity purification with an anti-Cas9...chromatin immunoprecipitation (enChIP)." Scientific reports 3 (2013): 3171. PubMed PMID: 24201379. PubMed Central...Find CRISPR plasmids by function Find recently deposited plasmids ...

...are removed and replaced by a single (non-viral) reporter gene (e.g. firefly luciferase or eGFP) flanked...necessary to direct minigenome replication and reporter gene transcription by the EBOV polymerase (reviewed...the minigenome as a template to transcribe the reporter gene (e.g. firefly luciferase). The polymerase...start signal and initiates transcription of the reporter gene. The polymerase will elongate the mRNA until...

...technology more user-friendly and precise. We will deposit the plasmids of these systems to Addgene in the...Scientific Community Addgene: In 2012 your lab first deposited plasmids with Addgene. In 2014, you joined Addgene's..., as well as the Editorial Board of Scientific Reports and PLoS One. Additionally, you are involved in...shopping for a wide range of plasmids. The merit of depositing plasmids to Addgene has been spectacular. We'...

...maintaining fluorescence. The protein structure, first reported in 1996, is an eleven β-sheet-containing “barrel...constructing fluorescent fusions. Transcription Reporter: Placing GFP under the control of a promoter of...a given cell type. This type of transcription reporting was among the earliest uses of GFP. Fluorescence...micrometastases in nude mice in cancer studies, act as a reporter for DNA double strand break repair, and to label...

...-founded by Harvard Investigators and Addgene depositors David Liu, PhD, Keith Joung, PhD, and Feng Zhang...Center will continue to expand as Rett scientists deposit their MECP2-related plasmid tools with Addgene ...Researchers can also find links to cell line repositories including patient-derived iPSCs, MECP2 animal...Neurological Disorder by Programmable RNA Editing. Cell Reports 32:107878 . https://doi.org/10.1016/j.celrep.2020.107878...

... extract as much information as possible from depositing laboratories and associated publications, but...would ‘spot-check’ critical regions of plasmids deposited with us via Sanger sequencing, while more recently...Generation Sequencing (NGS) technology. As our repository continues to expand, we realized the need to ...scale our lab work as we grew. As the number of deposited plasmids increased, the accompanying sequencing...

...pooled library.  Pooled libraries Scientists have deposited many types of pooled libraries here at Addgene... from the original library constructed by the depositing lab. (For our more popular libraries, you might...recommend following the protocols provided by the depositing lab whenever available. Addgene also shares the... other information or scripts provided by the depositing lab for data analysis.  I'm often asked whether...

...in the same lane, and the resulting fraction is reported. Figure 2: Normalization with loading controls...equally strong, so the normalized value simply reports which POI band is stronger. The right gel has exactly...abundance on your membrane, which would be directly reported by band intensity. Researchers use later steps...perturbed by the experiment Unfortunately, multiple reports suggest that common housekeeping proteins are influenced...