We narrowed to 388 results for: HAL

...generating the standard curve for qPCR can be challenging to get right. Here at Addgene, we use a linearized...droplet digital PCR for vaccine evaluation in challenged monkeys and TB diagnosis." Emerging microbes ...

...may provide a method for doing just that. While challenges remain in the delivery of these agents, CRISPR...CRISPR-induced double-stranded break (DSB) in the genome is lethal. If this DSB occurs on a plasmid, the plasmid will...

...purify proteins can face any number of technical challenges depending on their protein of interest. After...After several decades of trying to address these challenges, researchers have amassed a considerable molecular...

...transgenic hairy root lines in tissue cultures takes half a year, and generating a transgenic plant can take...Luciferases usually have shorter mRNA and protein half-lives then GUS and are therefore good temporal reporters...

...permissiveness. The first generation of outbred mice (F1) had half the level of CNS PHP.B transduction as their C57BL...check out these two references: Dayton, Robert D., Mychal S. Grames, and Ronald L. Klein. "More expansive...

...counter-intuitive, but I find office work more mentally challenging than lab work at times.” Sometimes a desk job...to non-scientists can be a fun and gratifying challenge. You’re also getting the opportunity to learn ...

...relationships. Personally, I find this to be the most challenging and time-intensive part of relationship building...research-intensive careers. In addition, this skill is a hallmark for jobs focusing on: Science Policy, Business...

...300,000 individual nucleotide sequences. More than half of these sequences were generated by Addgene as ...on-site, but now we were presented with a new challenge: automating the bioinformatics steps needed to...

...approximately 1000–1400 amino acids — which poses a challenge for efficient delivery into cells, particularly...Cas12a. Applications of TnpB One of the major challenges in genome editing is the efficient delivery of...

...from flow cytometry experiments can be a little challenging to understand if you’ve never done any flow. ...., Sait, K., Anfinan, N., Al-Wasiyah,  mohammad khalid, Huwait, E., Gari, M., & Al-Qahtani, M. (2018)....

...antibodies. Assay development, however, can be challenging since scientists must first identify two independent...Longer protocolIncreased chance of cross-reactivityChallenging to develop assay Competitive Appropriate...

...earlier Voltron indicators (a fusion of Ace2N and HaloTag, Figure 4), they determined that an A122D mutation...changing the dye that becomes conjugated with its HaloTag domain.  Find Voltron2 plasmids here! Abdelfattah...

...suited for recombinant protein expression. Many challenges can arise when over-expressing a foreign protein...functional RNaseE which results in longer transcript half-life F- ompT lon hsdSB (rB- mB-) gal dcm rne131...

...properties of the protein of interest can be challenging. In this blog I discuss strategies to generate... (catalytic activity or interactions) can be challenging. If a fusion can complement a knock-down or knock-out...

...even axons of up to 100 microns in length. Some challenges of the Brainbow system are that it does not use...Limbal Stem Cell Deficiency." Investigative ophthalmology & visual science 59.3 (2018): 1630-1640. Sakaguchi...

...double strand break repair, it has proven more challenging to develop high efficiency CRISPR-based approaches...delivering CRISPR components as RNPs and delivering two halves of a repair template spread across two AAVs, it...

... the body or self antigens, and toxins that are lethal to animals.  In the clinic, scFvs’ size also provides...plasmid in bacteria (Ahmed et al., 2012). One challenge of bacterial expression is the proper formation...

...base editing intervention was created. Only about half of babies with KJ’s disorder survive long enough...editing efficiency, and target access were all challenges for these early base editors. Further innovations...

...and significance, these receptors have proved challenging to study. Cells often express multiple integrins...adhesion. The results surprised the investigators, challenging the long-held belief that αVβ3 would be the most...

...experiment, but you’ll only spend half of each day on it. The other half of each day you can spend accomplishing...