Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 147 results for: INV-E

Showing: 141 - 147 of 147 results

  1. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    Published
    Oct. 11, 2016, 2:30 p.m.
    ...:947–951 . https://doi.org/10.1038/nbt.2969 Waltz E (2016) Gene-edited CRISPR mushroom escapes US regulation...homology directed repair, these methods do not involve double strand break (DSB) formation and do not ...

  2. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    Published
    July 28, 2016, 2:30 p.m.
    ...implement RSMs? In our study (1), we implement RSMs in E. coli with two plasmids (Figure 3 below): Input Plasmid...then the result of their recombination is the inversion of the DNA between them (Fig. 1A below). In the...

  3. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ...Bandrowski A, Carr S, Edwards A, Ellenberg J, Lundberg E, Rimm DL, Rodriguez H, Hiltke T, Snyder M, Yamamoto...- it is hard to distinguish between specific, invariable expression and non-specific, background signal...

  4. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ...Central PMCID: PMC3381869. 7. McCarty, D. M., Paul E. Monahan, and Richard J. Samulski. "Self-complementary...Drugs, DREADDs) and optogenetics as tools to investigate the roles of certain cell types in locomotion...cause the least amount of immune response. Any investigator who is using an in vivo animal model should ...

  5. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ...Engineering production of functional scFv antibody in E. coli by co-expressing the molecule chaperone Skp....lot of flexibility in which proteins you can investigate in each panel. An advantage of flow cytometry.... These applications are typically used for investigating signaling pathways or developing therapeutics...

  6. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    Published
    March 7, 2017, 3:30 p.m.
    ...-mediated gene inactivation. Doench JG, Hartenian E, Graham DB, Tothova Z, Hegde M, Smith I, Sullender...growing so slowly, there isn’t much maintenance involved. Also it was really fun to find individual colonies...

  7. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    Published
    Nov. 3, 2015, 3:30 p.m.
    ...parameters represent. The best learn-by-doing tool is E-CRISP. The authors have made a huge effort so that...shouldn’t be a surprise that the CRISPR system was involved. Today, that same laboratory enjoys a successful... a clear winner, is there something that we can invest our time into learning and extract the most value...
Showing: 141 - 147 of 147 results